Multilocus Sequence Typing of Genital Chlamydia trachomatis in Norway Reveals Multiple New Sequence Types and a Large Genetic Diversity
Gravningen's doctoral thesis, available in Munin at http://hdl.handle.net/10037/5649 The Chlamydia trachomatis incidence rate in Finnmark, the most northern and sparsely populated county in Norway, has been twice the national average. This population based cross-sectional study among Finnmark h...
Published in: | PLoS ONE |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2012
|
Subjects: | |
Online Access: | https://hdl.handle.net/10037/5019 https://doi.org/10.1371/journal.pone.0034452 |
id |
ftunivtroemsoe:oai:munin.uit.no:10037/5019 |
---|---|
record_format |
openpolar |
spelling |
ftunivtroemsoe:oai:munin.uit.no:10037/5019 2023-05-15T16:13:31+02:00 Multilocus Sequence Typing of Genital Chlamydia trachomatis in Norway Reveals Multiple New Sequence Types and a Large Genetic Diversity Gravningen, Kirsten Midttun Christerson, Linus Furberg, Anne-Sofie Simonsen, Gunnar Skov Ödman, Kristina Ståhlsten, Anna Herrmann, Björn 2012 https://hdl.handle.net/10037/5019 https://doi.org/10.1371/journal.pone.0034452 eng eng Public Library of Science (PLoS) PLoS ONE (2012), vol. 7(3): e34452 FRIDAID 960362 http://dx.doi.org/10.1371/journal.pone.0034452 1932-6203 https://hdl.handle.net/10037/5019 URN:NBN:no-uit_munin_4693 openAccess VDP::Medical disciplines: 700::Clinical medical disciplines: 750::Communicable diseases: 776 VDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Infeksjonsmedisin: 776 Journal article Tidsskriftartikkel Peer reviewed 2012 ftunivtroemsoe https://doi.org/10.1371/journal.pone.0034452 2021-06-25T17:53:30Z Gravningen's doctoral thesis, available in Munin at http://hdl.handle.net/10037/5649 The Chlamydia trachomatis incidence rate in Finnmark, the most northern and sparsely populated county in Norway, has been twice the national average. This population based cross-sectional study among Finnmark high school students had the following aims: i) to examine distribution of multilocus sequence types (STs) of C. trachomatis in a previously unmapped area, ii) to compare chlamydia genetic diversity in Finnmark with that of two urban regions, and iii) to compare discriminatory capacity of multilocus sequence typing (MLST) with conventional ompA sequencing in a large number of chlamydia specimens. ompA sequencing and a high-resolution MLST system based on PCR amplification and DNA sequencing of five highly variable genetic regions were used. Eighty chlamydia specimens from adolescents aged 15–20 years in Finnmark were collected in five high schools (n = 60) and from routine clinical samples in the laboratory (n = 20). These were compared to routine clinical samples from adolescents in Tromsø (n = 80) and Trondheim (n = 88), capitals of North and Central Norway, respectively. ompA sequencing detected 11 genotypes in 248 specimens from all three areas. MLST displayed 50 STs providing a five-fold higher resolution. Two-thirds of all STs were novel. The common ompA E/Bour genotype comprised 46% and resolved into 24 different STs. MLST identified the Swedish new variant of C. trachomatis not discriminated by ompA sequencing. Simpson's discriminatory index (D) was 0.93 for MLST, while a corrected Dc was 0.97. There were no statistically significant differences in ST genetic diversity between geographic areas. Finnmark had an atypical genovar distribution with G being predominant. This was mainly due to expansion of specific STs of which the novel ST161 was unique for Finnmark. MLST revealed multiple new STs and a larger genetic diversity in comparison to ompA sequencing and proved to be a useful tool in molecular epidemiology of chlamydia infections Article in Journal/Newspaper Finnmark Tromsø Finnmark University of Tromsø: Munin Open Research Archive Norway Tromsø PLoS ONE 7 3 e34452 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Medical disciplines: 700::Clinical medical disciplines: 750::Communicable diseases: 776 VDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Infeksjonsmedisin: 776 |
spellingShingle |
VDP::Medical disciplines: 700::Clinical medical disciplines: 750::Communicable diseases: 776 VDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Infeksjonsmedisin: 776 Gravningen, Kirsten Midttun Christerson, Linus Furberg, Anne-Sofie Simonsen, Gunnar Skov Ödman, Kristina Ståhlsten, Anna Herrmann, Björn Multilocus Sequence Typing of Genital Chlamydia trachomatis in Norway Reveals Multiple New Sequence Types and a Large Genetic Diversity |
topic_facet |
VDP::Medical disciplines: 700::Clinical medical disciplines: 750::Communicable diseases: 776 VDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Infeksjonsmedisin: 776 |
description |
Gravningen's doctoral thesis, available in Munin at http://hdl.handle.net/10037/5649 The Chlamydia trachomatis incidence rate in Finnmark, the most northern and sparsely populated county in Norway, has been twice the national average. This population based cross-sectional study among Finnmark high school students had the following aims: i) to examine distribution of multilocus sequence types (STs) of C. trachomatis in a previously unmapped area, ii) to compare chlamydia genetic diversity in Finnmark with that of two urban regions, and iii) to compare discriminatory capacity of multilocus sequence typing (MLST) with conventional ompA sequencing in a large number of chlamydia specimens. ompA sequencing and a high-resolution MLST system based on PCR amplification and DNA sequencing of five highly variable genetic regions were used. Eighty chlamydia specimens from adolescents aged 15–20 years in Finnmark were collected in five high schools (n = 60) and from routine clinical samples in the laboratory (n = 20). These were compared to routine clinical samples from adolescents in Tromsø (n = 80) and Trondheim (n = 88), capitals of North and Central Norway, respectively. ompA sequencing detected 11 genotypes in 248 specimens from all three areas. MLST displayed 50 STs providing a five-fold higher resolution. Two-thirds of all STs were novel. The common ompA E/Bour genotype comprised 46% and resolved into 24 different STs. MLST identified the Swedish new variant of C. trachomatis not discriminated by ompA sequencing. Simpson's discriminatory index (D) was 0.93 for MLST, while a corrected Dc was 0.97. There were no statistically significant differences in ST genetic diversity between geographic areas. Finnmark had an atypical genovar distribution with G being predominant. This was mainly due to expansion of specific STs of which the novel ST161 was unique for Finnmark. MLST revealed multiple new STs and a larger genetic diversity in comparison to ompA sequencing and proved to be a useful tool in molecular epidemiology of chlamydia infections |
format |
Article in Journal/Newspaper |
author |
Gravningen, Kirsten Midttun Christerson, Linus Furberg, Anne-Sofie Simonsen, Gunnar Skov Ödman, Kristina Ståhlsten, Anna Herrmann, Björn |
author_facet |
Gravningen, Kirsten Midttun Christerson, Linus Furberg, Anne-Sofie Simonsen, Gunnar Skov Ödman, Kristina Ståhlsten, Anna Herrmann, Björn |
author_sort |
Gravningen, Kirsten Midttun |
title |
Multilocus Sequence Typing of Genital Chlamydia trachomatis in Norway Reveals Multiple New Sequence Types and a Large Genetic Diversity |
title_short |
Multilocus Sequence Typing of Genital Chlamydia trachomatis in Norway Reveals Multiple New Sequence Types and a Large Genetic Diversity |
title_full |
Multilocus Sequence Typing of Genital Chlamydia trachomatis in Norway Reveals Multiple New Sequence Types and a Large Genetic Diversity |
title_fullStr |
Multilocus Sequence Typing of Genital Chlamydia trachomatis in Norway Reveals Multiple New Sequence Types and a Large Genetic Diversity |
title_full_unstemmed |
Multilocus Sequence Typing of Genital Chlamydia trachomatis in Norway Reveals Multiple New Sequence Types and a Large Genetic Diversity |
title_sort |
multilocus sequence typing of genital chlamydia trachomatis in norway reveals multiple new sequence types and a large genetic diversity |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://hdl.handle.net/10037/5019 https://doi.org/10.1371/journal.pone.0034452 |
geographic |
Norway Tromsø |
geographic_facet |
Norway Tromsø |
genre |
Finnmark Tromsø Finnmark |
genre_facet |
Finnmark Tromsø Finnmark |
op_relation |
PLoS ONE (2012), vol. 7(3): e34452 FRIDAID 960362 http://dx.doi.org/10.1371/journal.pone.0034452 1932-6203 https://hdl.handle.net/10037/5019 URN:NBN:no-uit_munin_4693 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0034452 |
container_title |
PLoS ONE |
container_volume |
7 |
container_issue |
3 |
container_start_page |
e34452 |
_version_ |
1765999249448763392 |