Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from Norway: evaluation of a PCR test targeting the chromosomal flaB gene
A consensus TaqMan real-time PCR test targeting the chromosomal flaB gene of Borrelia burgdorferi sensu lato was constructed. The test was compared with a recently published generic Light Upon eXtension (LUX) 16S rRNA real-time PCR test (Wilhelmsson et al. in J Clin Microbiol 48:4169–4176, 2010) on...
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Online Access: | https://hdl.handle.net/10037/4984 https://doi.org/10.1007/s10493-012-9585-2 |
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ftunivtroemsoe:oai:munin.uit.no:10037/4984 2023-05-15T17:43:21+02:00 Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from Norway: evaluation of a PCR test targeting the chromosomal flaB gene Jenkins, Andrew Hvidsten, Dag Matussek, Andreas Lindgren, Per-Eric Stuen, Snorre Kristiansen, Bjørn-Erik 2012 https://hdl.handle.net/10037/4984 https://doi.org/10.1007/s10493-012-9585-2 eng eng Experimental & applied acarology 58(2012) nr. 4 s. 431-439 FRIDAID 976106 http://dx.doi.org/10.1007/s10493-012-9585-2 0168-8162 https://hdl.handle.net/10037/4984 URN:NBN:no-uit_munin_4718 openAccess VDP::Medical disciplines: 700::Basic medical dental and veterinary science disciplines: 710::Medical genetics: 714 VDP::Medisinske Fag: 700::Basale medisinske odontologiske og veterinærmedisinske fag: 710::Medisinsk genetikk: 714 Journal article Tidsskriftartikkel Peer reviewed 2012 ftunivtroemsoe https://doi.org/10.1007/s10493-012-9585-2 2021-06-25T17:53:32Z A consensus TaqMan real-time PCR test targeting the chromosomal flaB gene of Borrelia burgdorferi sensu lato was constructed. The test was compared with a recently published generic Light Upon eXtension (LUX) 16S rRNA real-time PCR test (Wilhelmsson et al. in J Clin Microbiol 48:4169–4176, 2010) on material consisting of 242 Ixodes ricinus ticks collected from dogs and cats in Northern Norway (n = 139) and Telemark County in Southern Norway (n = 103). Ticks positive in either test were further tested by nested PCR amplification of the 5S-23S rRNA intergenic-spacer region followed by sequencing for species identification. A tick was defined as Borrelia positive if two of three tests were positive. Thirty-four of the 242 (14 %) ticks satisfied this definition of positivity. Of these ticks 32 were positive both in the rRNA and flaB test, while two were positive only in the rRNA test. One tick was positive only in the rRNA test and was considered false positive since PCR for sequencing failed. The sensitivity of the flaB test was 94 % and the specificity 100 %. It was possible to determine the species present using Tm analysis. Among ticks from Northern Norway the prevalence of Borrelia was 13 %, whereas the prevalence in Telemark was 16 %. Among identified species (n = 33) B. afzelii was found in 16 (47 %), B. garinii in 15 (44 %) and B. valaisiana in 2 (6 %) ticks, respectively. The flaB test is a rapid, sensitive and specific test for detection and quantification of Borrelia burgdorferi s.l. in I. ricinus ticks. This is the first report on Borrelia prevalence in I. ricinus in Northern Norway. Article in Journal/Newspaper Northern Norway University of Tromsø: Munin Open Research Archive Norway Experimental and Applied Acarology 58 4 431 439 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Medical disciplines: 700::Basic medical dental and veterinary science disciplines: 710::Medical genetics: 714 VDP::Medisinske Fag: 700::Basale medisinske odontologiske og veterinærmedisinske fag: 710::Medisinsk genetikk: 714 |
spellingShingle |
VDP::Medical disciplines: 700::Basic medical dental and veterinary science disciplines: 710::Medical genetics: 714 VDP::Medisinske Fag: 700::Basale medisinske odontologiske og veterinærmedisinske fag: 710::Medisinsk genetikk: 714 Jenkins, Andrew Hvidsten, Dag Matussek, Andreas Lindgren, Per-Eric Stuen, Snorre Kristiansen, Bjørn-Erik Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from Norway: evaluation of a PCR test targeting the chromosomal flaB gene |
topic_facet |
VDP::Medical disciplines: 700::Basic medical dental and veterinary science disciplines: 710::Medical genetics: 714 VDP::Medisinske Fag: 700::Basale medisinske odontologiske og veterinærmedisinske fag: 710::Medisinsk genetikk: 714 |
description |
A consensus TaqMan real-time PCR test targeting the chromosomal flaB gene of Borrelia burgdorferi sensu lato was constructed. The test was compared with a recently published generic Light Upon eXtension (LUX) 16S rRNA real-time PCR test (Wilhelmsson et al. in J Clin Microbiol 48:4169–4176, 2010) on material consisting of 242 Ixodes ricinus ticks collected from dogs and cats in Northern Norway (n = 139) and Telemark County in Southern Norway (n = 103). Ticks positive in either test were further tested by nested PCR amplification of the 5S-23S rRNA intergenic-spacer region followed by sequencing for species identification. A tick was defined as Borrelia positive if two of three tests were positive. Thirty-four of the 242 (14 %) ticks satisfied this definition of positivity. Of these ticks 32 were positive both in the rRNA and flaB test, while two were positive only in the rRNA test. One tick was positive only in the rRNA test and was considered false positive since PCR for sequencing failed. The sensitivity of the flaB test was 94 % and the specificity 100 %. It was possible to determine the species present using Tm analysis. Among ticks from Northern Norway the prevalence of Borrelia was 13 %, whereas the prevalence in Telemark was 16 %. Among identified species (n = 33) B. afzelii was found in 16 (47 %), B. garinii in 15 (44 %) and B. valaisiana in 2 (6 %) ticks, respectively. The flaB test is a rapid, sensitive and specific test for detection and quantification of Borrelia burgdorferi s.l. in I. ricinus ticks. This is the first report on Borrelia prevalence in I. ricinus in Northern Norway. |
format |
Article in Journal/Newspaper |
author |
Jenkins, Andrew Hvidsten, Dag Matussek, Andreas Lindgren, Per-Eric Stuen, Snorre Kristiansen, Bjørn-Erik |
author_facet |
Jenkins, Andrew Hvidsten, Dag Matussek, Andreas Lindgren, Per-Eric Stuen, Snorre Kristiansen, Bjørn-Erik |
author_sort |
Jenkins, Andrew |
title |
Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from Norway: evaluation of a PCR test targeting the chromosomal flaB gene |
title_short |
Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from Norway: evaluation of a PCR test targeting the chromosomal flaB gene |
title_full |
Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from Norway: evaluation of a PCR test targeting the chromosomal flaB gene |
title_fullStr |
Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from Norway: evaluation of a PCR test targeting the chromosomal flaB gene |
title_full_unstemmed |
Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from Norway: evaluation of a PCR test targeting the chromosomal flaB gene |
title_sort |
borrelia burgdorferi sensu lato in ixodes ricinus ticks from norway: evaluation of a pcr test targeting the chromosomal flab gene |
publishDate |
2012 |
url |
https://hdl.handle.net/10037/4984 https://doi.org/10.1007/s10493-012-9585-2 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Northern Norway |
genre_facet |
Northern Norway |
op_relation |
Experimental & applied acarology 58(2012) nr. 4 s. 431-439 FRIDAID 976106 http://dx.doi.org/10.1007/s10493-012-9585-2 0168-8162 https://hdl.handle.net/10037/4984 URN:NBN:no-uit_munin_4718 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1007/s10493-012-9585-2 |
container_title |
Experimental and Applied Acarology |
container_volume |
58 |
container_issue |
4 |
container_start_page |
431 |
op_container_end_page |
439 |
_version_ |
1766145397389000704 |