Histological and bacteriological changes in intestine of beluga (Huso huso) following ex vivo exposure to bacterial strains
In the present study the intestinal sac method (ex vivo) was used to evaluate the interactions between lactic acid bacteria and staphylococci in the gastrointestinal (GI) tract of beluga (Huso huso). The distal intestine (DI) of beluga was exposed ex vivo to Staphylococcus aureus, Leuconostoc mesent...
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2011
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Online Access: | https://hdl.handle.net/10037/4187 https://doi.org/10.1016/j.aquaculture.2011.01.047 |
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ftunivtroemsoe:oai:munin.uit.no:10037/4187 2023-05-15T15:41:28+02:00 Histological and bacteriological changes in intestine of beluga (Huso huso) following ex vivo exposure to bacterial strains Salma, Wahida Zhou, Zhigang Wang, Wenwan Askarian, Fatemeh Kousha, Armin Ebrahimi, Maryam Tajabadi Myklebust, Reidar Ringø, Einar 2011 https://hdl.handle.net/10037/4187 https://doi.org/10.1016/j.aquaculture.2011.01.047 eng eng Elsevier Aquaculture 314(2011) nr. 1-4 s. 24-33 FRIDAID 840082 doi:10.1016/j.aquaculture.2011.01.047 0044-8486 https://hdl.handle.net/10037/4187 URN:NBN:no-uit_munin_3904 openAccess VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Fiskehelse: 923 Journal article Tidsskriftartikkel Peer reviewed 2011 ftunivtroemsoe https://doi.org/10.1016/j.aquaculture.2011.01.047 2021-06-25T17:53:18Z In the present study the intestinal sac method (ex vivo) was used to evaluate the interactions between lactic acid bacteria and staphylococci in the gastrointestinal (GI) tract of beluga (Huso huso). The distal intestine (DI) of beluga was exposed ex vivo to Staphylococcus aureus, Leuconostoc mesenteroides and Lactobacillus plantarum. Histological changes following bacterial exposure were assessed by light and electron microscopy. Control samples and samples exposed only to L. mesenteroides and a combination of L. mesenteroides and S. aureus, had a similar appearance to intact intestinal mucosal epithelium, with no signs of cellular damage. However, the exposure of the DI to S. aureus and L. plantarum resulted in damaged epithelial cells and disorganised microvilli. Furthermore, 16S rDNA PCR denaturing gradient gel electrophoresis (PCR-DGGE) was used to investigate the adherent microbiota of distal beluga intestine. Several bacterial species were identified by DGGE in the present study that has not previously been identified in beluga. Article in Journal/Newspaper Beluga Beluga* University of Tromsø: Munin Open Research Archive Aquaculture 314 1-4 24 33 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Fiskehelse: 923 |
spellingShingle |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Fiskehelse: 923 Salma, Wahida Zhou, Zhigang Wang, Wenwan Askarian, Fatemeh Kousha, Armin Ebrahimi, Maryam Tajabadi Myklebust, Reidar Ringø, Einar Histological and bacteriological changes in intestine of beluga (Huso huso) following ex vivo exposure to bacterial strains |
topic_facet |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Fiskehelse: 923 |
description |
In the present study the intestinal sac method (ex vivo) was used to evaluate the interactions between lactic acid bacteria and staphylococci in the gastrointestinal (GI) tract of beluga (Huso huso). The distal intestine (DI) of beluga was exposed ex vivo to Staphylococcus aureus, Leuconostoc mesenteroides and Lactobacillus plantarum. Histological changes following bacterial exposure were assessed by light and electron microscopy. Control samples and samples exposed only to L. mesenteroides and a combination of L. mesenteroides and S. aureus, had a similar appearance to intact intestinal mucosal epithelium, with no signs of cellular damage. However, the exposure of the DI to S. aureus and L. plantarum resulted in damaged epithelial cells and disorganised microvilli. Furthermore, 16S rDNA PCR denaturing gradient gel electrophoresis (PCR-DGGE) was used to investigate the adherent microbiota of distal beluga intestine. Several bacterial species were identified by DGGE in the present study that has not previously been identified in beluga. |
format |
Article in Journal/Newspaper |
author |
Salma, Wahida Zhou, Zhigang Wang, Wenwan Askarian, Fatemeh Kousha, Armin Ebrahimi, Maryam Tajabadi Myklebust, Reidar Ringø, Einar |
author_facet |
Salma, Wahida Zhou, Zhigang Wang, Wenwan Askarian, Fatemeh Kousha, Armin Ebrahimi, Maryam Tajabadi Myklebust, Reidar Ringø, Einar |
author_sort |
Salma, Wahida |
title |
Histological and bacteriological changes in intestine of beluga (Huso huso) following ex vivo exposure to bacterial strains |
title_short |
Histological and bacteriological changes in intestine of beluga (Huso huso) following ex vivo exposure to bacterial strains |
title_full |
Histological and bacteriological changes in intestine of beluga (Huso huso) following ex vivo exposure to bacterial strains |
title_fullStr |
Histological and bacteriological changes in intestine of beluga (Huso huso) following ex vivo exposure to bacterial strains |
title_full_unstemmed |
Histological and bacteriological changes in intestine of beluga (Huso huso) following ex vivo exposure to bacterial strains |
title_sort |
histological and bacteriological changes in intestine of beluga (huso huso) following ex vivo exposure to bacterial strains |
publisher |
Elsevier |
publishDate |
2011 |
url |
https://hdl.handle.net/10037/4187 https://doi.org/10.1016/j.aquaculture.2011.01.047 |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_relation |
Aquaculture 314(2011) nr. 1-4 s. 24-33 FRIDAID 840082 doi:10.1016/j.aquaculture.2011.01.047 0044-8486 https://hdl.handle.net/10037/4187 URN:NBN:no-uit_munin_3904 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1016/j.aquaculture.2011.01.047 |
container_title |
Aquaculture |
container_volume |
314 |
container_issue |
1-4 |
container_start_page |
24 |
op_container_end_page |
33 |
_version_ |
1766374372616962048 |