Characterization of mitochondrial mRNAs in codfish reveals unique features compared to mammals
Expression and processing of mitochondrial gene transcripts are fundamental to mitochondrial function, but information from early vertebrates like teleost fishes is essentially lacking. We have analyzed mitogenome sequences of ten codfishes (family Gadidae), and provide complete sequences from three...
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ftunivtroemsoe:oai:munin.uit.no:10037/4039 2023-05-15T15:27:39+02:00 Characterization of mitochondrial mRNAs in codfish reveals unique features compared to mammals Coucheron, Dag-Hugo Nymark, Marianne Breines, Ragna Karlsen, Bård Ove Andreassen, morten Jørgensen, Tor Erik Moum, Truls Johansen, Steinar Daae 2011 https://hdl.handle.net/10037/4039 https://doi.org/10.1007/s00294-011-0338-2 eng eng Springer Berlin/Heidelberg Current Genetics 57(2011) nr. 3 s. 213-222 FRIDAID 834697 doi:10.1007/s00294-011-0338-2 0172-8083 https://hdl.handle.net/10037/4039 URN:NBN:no-uit_munin_3760 openAccess VDP::Mathematics and natural science: 400::Basic biosciences: 470::Molecular biology: 473 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Molekylærbiologi: 473 Journal article Tidsskriftartikkel Peer reviewed 2011 ftunivtroemsoe https://doi.org/10.1007/s00294-011-0338-2 2021-06-25T17:53:15Z Expression and processing of mitochondrial gene transcripts are fundamental to mitochondrial function, but information from early vertebrates like teleost fishes is essentially lacking. We have analyzed mitogenome sequences of ten codfishes (family Gadidae), and provide complete sequences from three new species (Saithe, Pollack and Blue whiting). Characterization of the mitochondrial mRNAs in Saithe and Atlantic cod identified a set of ten poly(A) transcripts, and six UAA stop codons are generated by posttranscriptional polyadenylation. Structural assessment of poly(A) sites is consistent with an RNaseP cleavage activity 5′ of tRNA acceptor-like stems. COI, ND5 and ND6 mRNAs were found to harbor 3′ UTRs with antisense potential extending into neighboring gene regions. While the 3′ UTR of COI mRNA is complementary to the tRNASer (UCN) and highly similar to that detected in human mitochondria, the ND5 and ND6 3′ UTRs appear more heterogenic. Deep sequencing confirms expression of all mitochondrial mRNAs and rRNAs, and provides information about the precise 5′ ends in mature transcripts. Our study supports an overall evolutionary conservation in mitochondrial RNA processing events among vertebrates, but reveals some unique 5′ and 3′ end characteristics in codfish mRNAs with implications to antisense regulation of gene expression. Article in Journal/Newspaper atlantic cod University of Tromsø: Munin Open Research Archive Current Genetics 57 3 213 222 |
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Open Polar |
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University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Mathematics and natural science: 400::Basic biosciences: 470::Molecular biology: 473 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Molekylærbiologi: 473 |
spellingShingle |
VDP::Mathematics and natural science: 400::Basic biosciences: 470::Molecular biology: 473 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Molekylærbiologi: 473 Coucheron, Dag-Hugo Nymark, Marianne Breines, Ragna Karlsen, Bård Ove Andreassen, morten Jørgensen, Tor Erik Moum, Truls Johansen, Steinar Daae Characterization of mitochondrial mRNAs in codfish reveals unique features compared to mammals |
topic_facet |
VDP::Mathematics and natural science: 400::Basic biosciences: 470::Molecular biology: 473 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Molekylærbiologi: 473 |
description |
Expression and processing of mitochondrial gene transcripts are fundamental to mitochondrial function, but information from early vertebrates like teleost fishes is essentially lacking. We have analyzed mitogenome sequences of ten codfishes (family Gadidae), and provide complete sequences from three new species (Saithe, Pollack and Blue whiting). Characterization of the mitochondrial mRNAs in Saithe and Atlantic cod identified a set of ten poly(A) transcripts, and six UAA stop codons are generated by posttranscriptional polyadenylation. Structural assessment of poly(A) sites is consistent with an RNaseP cleavage activity 5′ of tRNA acceptor-like stems. COI, ND5 and ND6 mRNAs were found to harbor 3′ UTRs with antisense potential extending into neighboring gene regions. While the 3′ UTR of COI mRNA is complementary to the tRNASer (UCN) and highly similar to that detected in human mitochondria, the ND5 and ND6 3′ UTRs appear more heterogenic. Deep sequencing confirms expression of all mitochondrial mRNAs and rRNAs, and provides information about the precise 5′ ends in mature transcripts. Our study supports an overall evolutionary conservation in mitochondrial RNA processing events among vertebrates, but reveals some unique 5′ and 3′ end characteristics in codfish mRNAs with implications to antisense regulation of gene expression. |
format |
Article in Journal/Newspaper |
author |
Coucheron, Dag-Hugo Nymark, Marianne Breines, Ragna Karlsen, Bård Ove Andreassen, morten Jørgensen, Tor Erik Moum, Truls Johansen, Steinar Daae |
author_facet |
Coucheron, Dag-Hugo Nymark, Marianne Breines, Ragna Karlsen, Bård Ove Andreassen, morten Jørgensen, Tor Erik Moum, Truls Johansen, Steinar Daae |
author_sort |
Coucheron, Dag-Hugo |
title |
Characterization of mitochondrial mRNAs in codfish reveals unique features compared to mammals |
title_short |
Characterization of mitochondrial mRNAs in codfish reveals unique features compared to mammals |
title_full |
Characterization of mitochondrial mRNAs in codfish reveals unique features compared to mammals |
title_fullStr |
Characterization of mitochondrial mRNAs in codfish reveals unique features compared to mammals |
title_full_unstemmed |
Characterization of mitochondrial mRNAs in codfish reveals unique features compared to mammals |
title_sort |
characterization of mitochondrial mrnas in codfish reveals unique features compared to mammals |
publisher |
Springer Berlin/Heidelberg |
publishDate |
2011 |
url |
https://hdl.handle.net/10037/4039 https://doi.org/10.1007/s00294-011-0338-2 |
genre |
atlantic cod |
genre_facet |
atlantic cod |
op_relation |
Current Genetics 57(2011) nr. 3 s. 213-222 FRIDAID 834697 doi:10.1007/s00294-011-0338-2 0172-8083 https://hdl.handle.net/10037/4039 URN:NBN:no-uit_munin_3760 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1007/s00294-011-0338-2 |
container_title |
Current Genetics |
container_volume |
57 |
container_issue |
3 |
container_start_page |
213 |
op_container_end_page |
222 |
_version_ |
1766358074036060160 |