Purification and characterization of a salt tolerant metallo-beta-lactamase from Aliivibrio salmonicida

• Main Author: Kristiansen, Anders
• Format: Master Thesis
• Language: English
• Published: Universitetet i Tromsø 2011
• Subjects: VDP::Matematikk og Naturvitenskap: 400::Kjemi: 440; VDP::Mathematics and natural science: 400::Chemistry: 440; Marine environment adaption; salt tolerance; nitrocefin stability; enzyme characterization; purification; metallo-beta-lactamase; temperature stability; experimental parameters; enzyme kinetics; Aliivibrio salmonicida; KJE-3900; Hitra; Atlantic salmon; Salmo salar
• Online Access: https://hdl.handle.net/10037/3684

Beta-lactamases are enzymes that inactivate beta-lactam antibiotics by hydrolyzing the amide bond that exists in the beta-lactam ring, disrupting the ring structure and make the antibiotics nonfunctional against bacteria. Metallo-beta-lactamases (MBLs) are a group of beta-lactamases which needs meta...