Mass cultivation of the marine diatom Porosira glacialis: Method development and comparison of pigment composition in response to irradiance wavelenght

In 2015, UiT The Arctic University of Norway and a local smelting plant initiated a project aiming to reduce the carbon footprint by mass cultivation of microalgae. The ability of the microalgae to directly capture industrially emitted CO2 and utilize wastewater as alternative nutrient sources to pr...

Full description

Bibliographic Details
Main Author: Bæverud, Agnethe Hansen
Format: Master Thesis
Language:English
Published: UiT Norges arktiske universitet 2022
Subjects:
Online Access:https://hdl.handle.net/10037/33584
id ftunivtroemsoe:oai:munin.uit.no:10037/33584
record_format openpolar
spelling ftunivtroemsoe:oai:munin.uit.no:10037/33584 2024-06-23T07:57:29+00:00 Mass cultivation of the marine diatom Porosira glacialis: Method development and comparison of pigment composition in response to irradiance wavelenght Bæverud, Agnethe Hansen 2022-05-19 https://hdl.handle.net/10037/33584 eng eng UiT Norges arktiske universitet UiT The Arctic University of Norway https://hdl.handle.net/10037/33584 Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) openAccess Copyright 2022 The Author(s) https://creativecommons.org/licenses/by-nc-sa/4.0 VDP::Matematikk og Naturvitenskap: 400::Kjemi: 440::Analytisk kjemi: 445 VDP::Mathematics and natural science: 400::Chemistry: 440::Analytical chemistry: 445 Pigment Light irradiance Microalgae Orbitrap UPLC-MS AMRT database FAR-3911 Master thesis Mastergradsoppgave 2022 ftunivtroemsoe 2024-05-29T00:47:55Z In 2015, UiT The Arctic University of Norway and a local smelting plant initiated a project aiming to reduce the carbon footprint by mass cultivation of microalgae. The ability of the microalgae to directly capture industrially emitted CO2 and utilize wastewater as alternative nutrient sources to produce high value biomass, causes the bio-carbon recycling to represent both an environmental and economical feasible strategy. The produced microalgal biomass composes value-added products as lipids, proteins and pigments, and has the potential to ensure macronutrient quality as supplements in aquaculture fish-feed. The main goals of this thesis were to develop a method for pigment analysis and investigate the pigment composition in response to irradiance wavelength. The polar diatom Porosira glacialis was cultivated under three different wavelength irradiances, red, blue and white light. Pigments were extracted from lyophilized algal biomass by utilization of a developed pigment extraction protocol and analyzed through HPLC-MS with positive ESI ionization. Data dependent acquisition (DDA) methods were used in order to create an in-house AMRT database, acquire identification information and determine relative pigment abundance. The identification and relative quantification were performed both manually and with the assistance of the software Compound Discoverer™. Master Thesis Arctic University of Norway UiT The Arctic University of Norway University of Tromsø: Munin Open Research Archive Arctic Norway
institution Open Polar
collection University of Tromsø: Munin Open Research Archive
op_collection_id ftunivtroemsoe
language English
topic VDP::Matematikk og Naturvitenskap: 400::Kjemi: 440::Analytisk kjemi: 445
VDP::Mathematics and natural science: 400::Chemistry: 440::Analytical chemistry: 445
Pigment
Light irradiance
Microalgae
Orbitrap
UPLC-MS
AMRT database
FAR-3911
spellingShingle VDP::Matematikk og Naturvitenskap: 400::Kjemi: 440::Analytisk kjemi: 445
VDP::Mathematics and natural science: 400::Chemistry: 440::Analytical chemistry: 445
Pigment
Light irradiance
Microalgae
Orbitrap
UPLC-MS
AMRT database
FAR-3911
Bæverud, Agnethe Hansen
Mass cultivation of the marine diatom Porosira glacialis: Method development and comparison of pigment composition in response to irradiance wavelenght
topic_facet VDP::Matematikk og Naturvitenskap: 400::Kjemi: 440::Analytisk kjemi: 445
VDP::Mathematics and natural science: 400::Chemistry: 440::Analytical chemistry: 445
Pigment
Light irradiance
Microalgae
Orbitrap
UPLC-MS
AMRT database
FAR-3911
description In 2015, UiT The Arctic University of Norway and a local smelting plant initiated a project aiming to reduce the carbon footprint by mass cultivation of microalgae. The ability of the microalgae to directly capture industrially emitted CO2 and utilize wastewater as alternative nutrient sources to produce high value biomass, causes the bio-carbon recycling to represent both an environmental and economical feasible strategy. The produced microalgal biomass composes value-added products as lipids, proteins and pigments, and has the potential to ensure macronutrient quality as supplements in aquaculture fish-feed. The main goals of this thesis were to develop a method for pigment analysis and investigate the pigment composition in response to irradiance wavelength. The polar diatom Porosira glacialis was cultivated under three different wavelength irradiances, red, blue and white light. Pigments were extracted from lyophilized algal biomass by utilization of a developed pigment extraction protocol and analyzed through HPLC-MS with positive ESI ionization. Data dependent acquisition (DDA) methods were used in order to create an in-house AMRT database, acquire identification information and determine relative pigment abundance. The identification and relative quantification were performed both manually and with the assistance of the software Compound Discoverer™.
format Master Thesis
author Bæverud, Agnethe Hansen
author_facet Bæverud, Agnethe Hansen
author_sort Bæverud, Agnethe Hansen
title Mass cultivation of the marine diatom Porosira glacialis: Method development and comparison of pigment composition in response to irradiance wavelenght
title_short Mass cultivation of the marine diatom Porosira glacialis: Method development and comparison of pigment composition in response to irradiance wavelenght
title_full Mass cultivation of the marine diatom Porosira glacialis: Method development and comparison of pigment composition in response to irradiance wavelenght
title_fullStr Mass cultivation of the marine diatom Porosira glacialis: Method development and comparison of pigment composition in response to irradiance wavelenght
title_full_unstemmed Mass cultivation of the marine diatom Porosira glacialis: Method development and comparison of pigment composition in response to irradiance wavelenght
title_sort mass cultivation of the marine diatom porosira glacialis: method development and comparison of pigment composition in response to irradiance wavelenght
publisher UiT Norges arktiske universitet
publishDate 2022
url https://hdl.handle.net/10037/33584
geographic Arctic
Norway
geographic_facet Arctic
Norway
genre Arctic University of Norway
UiT The Arctic University of Norway
genre_facet Arctic University of Norway
UiT The Arctic University of Norway
op_relation https://hdl.handle.net/10037/33584
op_rights Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
openAccess
Copyright 2022 The Author(s)
https://creativecommons.org/licenses/by-nc-sa/4.0
_version_ 1802651154280087552