Mass cultivation of the marine diatom Porosira glacialis: Method development and comparison of pigment composition in response to irradiance wavelenght
In 2015, UiT The Arctic University of Norway and a local smelting plant initiated a project aiming to reduce the carbon footprint by mass cultivation of microalgae. The ability of the microalgae to directly capture industrially emitted CO2 and utilize wastewater as alternative nutrient sources to pr...
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UiT Norges arktiske universitet
2022
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Online Access: | https://hdl.handle.net/10037/33584 |
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ftunivtroemsoe:oai:munin.uit.no:10037/33584 2024-06-23T07:57:29+00:00 Mass cultivation of the marine diatom Porosira glacialis: Method development and comparison of pigment composition in response to irradiance wavelenght Bæverud, Agnethe Hansen 2022-05-19 https://hdl.handle.net/10037/33584 eng eng UiT Norges arktiske universitet UiT The Arctic University of Norway https://hdl.handle.net/10037/33584 Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) openAccess Copyright 2022 The Author(s) https://creativecommons.org/licenses/by-nc-sa/4.0 VDP::Matematikk og Naturvitenskap: 400::Kjemi: 440::Analytisk kjemi: 445 VDP::Mathematics and natural science: 400::Chemistry: 440::Analytical chemistry: 445 Pigment Light irradiance Microalgae Orbitrap UPLC-MS AMRT database FAR-3911 Master thesis Mastergradsoppgave 2022 ftunivtroemsoe 2024-05-29T00:47:55Z In 2015, UiT The Arctic University of Norway and a local smelting plant initiated a project aiming to reduce the carbon footprint by mass cultivation of microalgae. The ability of the microalgae to directly capture industrially emitted CO2 and utilize wastewater as alternative nutrient sources to produce high value biomass, causes the bio-carbon recycling to represent both an environmental and economical feasible strategy. The produced microalgal biomass composes value-added products as lipids, proteins and pigments, and has the potential to ensure macronutrient quality as supplements in aquaculture fish-feed. The main goals of this thesis were to develop a method for pigment analysis and investigate the pigment composition in response to irradiance wavelength. The polar diatom Porosira glacialis was cultivated under three different wavelength irradiances, red, blue and white light. Pigments were extracted from lyophilized algal biomass by utilization of a developed pigment extraction protocol and analyzed through HPLC-MS with positive ESI ionization. Data dependent acquisition (DDA) methods were used in order to create an in-house AMRT database, acquire identification information and determine relative pigment abundance. The identification and relative quantification were performed both manually and with the assistance of the software Compound Discoverer™. Master Thesis Arctic University of Norway UiT The Arctic University of Norway University of Tromsø: Munin Open Research Archive Arctic Norway |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Matematikk og Naturvitenskap: 400::Kjemi: 440::Analytisk kjemi: 445 VDP::Mathematics and natural science: 400::Chemistry: 440::Analytical chemistry: 445 Pigment Light irradiance Microalgae Orbitrap UPLC-MS AMRT database FAR-3911 |
spellingShingle |
VDP::Matematikk og Naturvitenskap: 400::Kjemi: 440::Analytisk kjemi: 445 VDP::Mathematics and natural science: 400::Chemistry: 440::Analytical chemistry: 445 Pigment Light irradiance Microalgae Orbitrap UPLC-MS AMRT database FAR-3911 Bæverud, Agnethe Hansen Mass cultivation of the marine diatom Porosira glacialis: Method development and comparison of pigment composition in response to irradiance wavelenght |
topic_facet |
VDP::Matematikk og Naturvitenskap: 400::Kjemi: 440::Analytisk kjemi: 445 VDP::Mathematics and natural science: 400::Chemistry: 440::Analytical chemistry: 445 Pigment Light irradiance Microalgae Orbitrap UPLC-MS AMRT database FAR-3911 |
description |
In 2015, UiT The Arctic University of Norway and a local smelting plant initiated a project aiming to reduce the carbon footprint by mass cultivation of microalgae. The ability of the microalgae to directly capture industrially emitted CO2 and utilize wastewater as alternative nutrient sources to produce high value biomass, causes the bio-carbon recycling to represent both an environmental and economical feasible strategy. The produced microalgal biomass composes value-added products as lipids, proteins and pigments, and has the potential to ensure macronutrient quality as supplements in aquaculture fish-feed. The main goals of this thesis were to develop a method for pigment analysis and investigate the pigment composition in response to irradiance wavelength. The polar diatom Porosira glacialis was cultivated under three different wavelength irradiances, red, blue and white light. Pigments were extracted from lyophilized algal biomass by utilization of a developed pigment extraction protocol and analyzed through HPLC-MS with positive ESI ionization. Data dependent acquisition (DDA) methods were used in order to create an in-house AMRT database, acquire identification information and determine relative pigment abundance. The identification and relative quantification were performed both manually and with the assistance of the software Compound Discoverer™. |
format |
Master Thesis |
author |
Bæverud, Agnethe Hansen |
author_facet |
Bæverud, Agnethe Hansen |
author_sort |
Bæverud, Agnethe Hansen |
title |
Mass cultivation of the marine diatom Porosira glacialis: Method development and comparison of pigment composition in response to irradiance wavelenght |
title_short |
Mass cultivation of the marine diatom Porosira glacialis: Method development and comparison of pigment composition in response to irradiance wavelenght |
title_full |
Mass cultivation of the marine diatom Porosira glacialis: Method development and comparison of pigment composition in response to irradiance wavelenght |
title_fullStr |
Mass cultivation of the marine diatom Porosira glacialis: Method development and comparison of pigment composition in response to irradiance wavelenght |
title_full_unstemmed |
Mass cultivation of the marine diatom Porosira glacialis: Method development and comparison of pigment composition in response to irradiance wavelenght |
title_sort |
mass cultivation of the marine diatom porosira glacialis: method development and comparison of pigment composition in response to irradiance wavelenght |
publisher |
UiT Norges arktiske universitet |
publishDate |
2022 |
url |
https://hdl.handle.net/10037/33584 |
geographic |
Arctic Norway |
geographic_facet |
Arctic Norway |
genre |
Arctic University of Norway UiT The Arctic University of Norway |
genre_facet |
Arctic University of Norway UiT The Arctic University of Norway |
op_relation |
https://hdl.handle.net/10037/33584 |
op_rights |
Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) openAccess Copyright 2022 The Author(s) https://creativecommons.org/licenses/by-nc-sa/4.0 |
_version_ |
1802651154280087552 |