Evaluation of ozone and peracetic acid use during a Yersinia ruckeri challenge in Atlantic salmon (Salmo salar) freshwater recirculating aquaculture systems
Yersinia ruckeri (Y. ruckeri) the causative agent of Enteric red-mouth disease (ERM), a serious septicemic bacterial disease of salmonid fish, poses a significant challenge in salmon production. With the emergence of recirculating aquaculture system (RAS) in Norway, new biological challenges in rela...
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UiT The Arctic University of Norway
2023
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ftunivtroemsoe:oai:munin.uit.no:10037/33571 2024-06-23T07:51:16+00:00 Evaluation of ozone and peracetic acid use during a Yersinia ruckeri challenge in Atlantic salmon (Salmo salar) freshwater recirculating aquaculture systems Mousavi, Samaneh 2023-05-16 https://hdl.handle.net/10037/33571 eng eng UiT The Arctic University of Norway UiT Norges arktiske universitet https://hdl.handle.net/10037/33571 Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) Copyright 2023 The Author(s) https://creativecommons.org/licenses/by-nc-sa/4.0 Atlantic salmon recirculating aquaculture system ozone peracetic acid Yersinia ruckeri fish health and welfare water quality BIO-3955 Master thesis Mastergradsoppgave 2023 ftunivtroemsoe 2024-05-29T00:47:55Z Yersinia ruckeri (Y. ruckeri) the causative agent of Enteric red-mouth disease (ERM), a serious septicemic bacterial disease of salmonid fish, poses a significant challenge in salmon production. With the emergence of recirculating aquaculture system (RAS) in Norway, new biological challenges in relation to infectious disease outbreaks have arisen. There is a gap in knowledge on how potential disinfection methods in the rearing water could help reduce or eliminate the risk of infectious disease outbreaks in RAS. Ozone and peracetic acid (PAA) are two disinfectants used in aquaculture. Both are reported to inactivate a range of pathogens, including Y. ruckeri. There are however limitations to the use of disinfectants in RAS as the re-use of water leads to accumulation of substances added to the water, and an ideal disinfectant need to balance pathogen control, fish health and welfare and biofilter performance. This study investigates the use of continuous ozone and semi-continuous PAA addition in RAS as a tool for preventing a disease outbreak of Y. ruckeri in Atlantic salmon parr. Nine replicated RAS units were stocked with Atlantic salmon parr (N=1800, ±19 g) and were treated with PAA (50.88 ml PAA product dose of 0.05% of make-up water daily), ozone (ORP: 500mV through protein skinner) or no treatment (control), in triplicates (n=3). To induce a ERM outbreak, Y. ruckeri was introduced through the make-up water. Health and welfare of fish were followed for 28 days after treatment started and 20 days after pathogen challenge, where water quality was monitored to see the effect of disinfectants on different water quality parameters. Although Y. ruckeri introduced to the systems did not lead to significant mortalities in any treatment group, and only mild clinical signs of the disease were observed, the pathogen was detected in the spleen of fish across all treatment groups. A trend could be seen towards higher infection prevalence in the control group, but no significant difference among the treatments (control: ... Master Thesis Atlantic salmon Salmo salar University of Tromsø: Munin Open Research Archive Norway Paa ENVELOPE(-53.483,-53.483,66.017,66.017) |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
Atlantic salmon recirculating aquaculture system ozone peracetic acid Yersinia ruckeri fish health and welfare water quality BIO-3955 |
spellingShingle |
Atlantic salmon recirculating aquaculture system ozone peracetic acid Yersinia ruckeri fish health and welfare water quality BIO-3955 Mousavi, Samaneh Evaluation of ozone and peracetic acid use during a Yersinia ruckeri challenge in Atlantic salmon (Salmo salar) freshwater recirculating aquaculture systems |
topic_facet |
Atlantic salmon recirculating aquaculture system ozone peracetic acid Yersinia ruckeri fish health and welfare water quality BIO-3955 |
description |
Yersinia ruckeri (Y. ruckeri) the causative agent of Enteric red-mouth disease (ERM), a serious septicemic bacterial disease of salmonid fish, poses a significant challenge in salmon production. With the emergence of recirculating aquaculture system (RAS) in Norway, new biological challenges in relation to infectious disease outbreaks have arisen. There is a gap in knowledge on how potential disinfection methods in the rearing water could help reduce or eliminate the risk of infectious disease outbreaks in RAS. Ozone and peracetic acid (PAA) are two disinfectants used in aquaculture. Both are reported to inactivate a range of pathogens, including Y. ruckeri. There are however limitations to the use of disinfectants in RAS as the re-use of water leads to accumulation of substances added to the water, and an ideal disinfectant need to balance pathogen control, fish health and welfare and biofilter performance. This study investigates the use of continuous ozone and semi-continuous PAA addition in RAS as a tool for preventing a disease outbreak of Y. ruckeri in Atlantic salmon parr. Nine replicated RAS units were stocked with Atlantic salmon parr (N=1800, ±19 g) and were treated with PAA (50.88 ml PAA product dose of 0.05% of make-up water daily), ozone (ORP: 500mV through protein skinner) or no treatment (control), in triplicates (n=3). To induce a ERM outbreak, Y. ruckeri was introduced through the make-up water. Health and welfare of fish were followed for 28 days after treatment started and 20 days after pathogen challenge, where water quality was monitored to see the effect of disinfectants on different water quality parameters. Although Y. ruckeri introduced to the systems did not lead to significant mortalities in any treatment group, and only mild clinical signs of the disease were observed, the pathogen was detected in the spleen of fish across all treatment groups. A trend could be seen towards higher infection prevalence in the control group, but no significant difference among the treatments (control: ... |
format |
Master Thesis |
author |
Mousavi, Samaneh |
author_facet |
Mousavi, Samaneh |
author_sort |
Mousavi, Samaneh |
title |
Evaluation of ozone and peracetic acid use during a Yersinia ruckeri challenge in Atlantic salmon (Salmo salar) freshwater recirculating aquaculture systems |
title_short |
Evaluation of ozone and peracetic acid use during a Yersinia ruckeri challenge in Atlantic salmon (Salmo salar) freshwater recirculating aquaculture systems |
title_full |
Evaluation of ozone and peracetic acid use during a Yersinia ruckeri challenge in Atlantic salmon (Salmo salar) freshwater recirculating aquaculture systems |
title_fullStr |
Evaluation of ozone and peracetic acid use during a Yersinia ruckeri challenge in Atlantic salmon (Salmo salar) freshwater recirculating aquaculture systems |
title_full_unstemmed |
Evaluation of ozone and peracetic acid use during a Yersinia ruckeri challenge in Atlantic salmon (Salmo salar) freshwater recirculating aquaculture systems |
title_sort |
evaluation of ozone and peracetic acid use during a yersinia ruckeri challenge in atlantic salmon (salmo salar) freshwater recirculating aquaculture systems |
publisher |
UiT The Arctic University of Norway |
publishDate |
2023 |
url |
https://hdl.handle.net/10037/33571 |
long_lat |
ENVELOPE(-53.483,-53.483,66.017,66.017) |
geographic |
Norway Paa |
geographic_facet |
Norway Paa |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
https://hdl.handle.net/10037/33571 |
op_rights |
Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) Copyright 2023 The Author(s) https://creativecommons.org/licenses/by-nc-sa/4.0 |
_version_ |
1802642302375559168 |