Gut microbiome biogeography in reindeer supersedes millennia of ecological and evolutionary separation
Ruminants are dependent on their gut microbiomes for nutrient extraction from plant diets. However, knowledge about the composition, diversity, function, and spatial structure of gut microbiomes, especially in wild ruminants, is limited, largely because analysis has been restricted to faeces or the...
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Online Access: | https://hdl.handle.net/10037/32290 https://doi.org/10.1093/femsec/fiad157 |
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ftunivtroemsoe:oai:munin.uit.no:10037/32290 2024-01-28T10:02:27+01:00 Gut microbiome biogeography in reindeer supersedes millennia of ecological and evolutionary separation Kamenova, Stefaniya de Muinck, Eric Veiberg, Vebjørn Utsi, Tove Hilde Ågnes Steyaert, Sam Albon, Steve Loe, Leif Egil Trosvik, Pål 2023-11-29 https://hdl.handle.net/10037/32290 https://doi.org/10.1093/femsec/fiad157 eng eng Oxford University Press FEMS Microbiology Ecology info:eu-repo/grantAgreement/EC/H2020/869471/EU/Drivers and Feedbacks of Changes in Arctic Terrestrial Biodiversity/CHARTER/ Kamenova S, de Muinck E, Veiberg V, Utsi TA, Steyaert SM, Albon S, Loe LE, Trosvik P. Gut microbiome biogeography in reindeer supersedes millennia of ecological and evolutionary separation. FEMS Microbiology Ecology. 2023;99(12) FRIDAID 2209851 doi:10.1093/femsec/fiad157 0168-6496 1574-6941 https://hdl.handle.net/10037/32290 Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) openAccess Copyright 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0 Journal article Tidsskriftartikkel Peer reviewed publishedVersion 2023 ftunivtroemsoe https://doi.org/10.1093/femsec/fiad157 2024-01-04T00:08:06Z Ruminants are dependent on their gut microbiomes for nutrient extraction from plant diets. However, knowledge about the composition, diversity, function, and spatial structure of gut microbiomes, especially in wild ruminants, is limited, largely because analysis has been restricted to faeces or the rumen. In two geographically separated reindeer subspecies, 16S rRNA gene amplicon sequencing revealed strong spatial structuring, and pronounced differences in microbial diversity of at least 33 phyla across the stomach, small intestine, and large intestine (including faeces). The main structural feature was the Bacteroidota to Firmicutes ratio, which declined from the stomach to the large intestine, likely reflecting functional adaptation. Metagenome shotgun sequencing also revealed highly significant structuring in the relative occurrence of carbohydrate-active enzymes (CAZymes). CAZymes were enriched in the rumen relative to the small and large intestines. Interestingly, taxonomic diversity was highest in the large intestine, suggesting an important and understudied role for this organ. Despite the two study populations being separated by an ocean and six millennia of evolutionary history, gut microbiome structuring was remarkably consistent. Our study suggests a strong selection for gut microbiome biogeography along the gastrointestinal tract in reindeer subspecies. Article in Journal/Newspaper Arctic University of Tromsø: Munin Open Research Archive FEMS Microbiology Ecology 99 12 |
institution |
Open Polar |
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University of Tromsø: Munin Open Research Archive |
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ftunivtroemsoe |
language |
English |
description |
Ruminants are dependent on their gut microbiomes for nutrient extraction from plant diets. However, knowledge about the composition, diversity, function, and spatial structure of gut microbiomes, especially in wild ruminants, is limited, largely because analysis has been restricted to faeces or the rumen. In two geographically separated reindeer subspecies, 16S rRNA gene amplicon sequencing revealed strong spatial structuring, and pronounced differences in microbial diversity of at least 33 phyla across the stomach, small intestine, and large intestine (including faeces). The main structural feature was the Bacteroidota to Firmicutes ratio, which declined from the stomach to the large intestine, likely reflecting functional adaptation. Metagenome shotgun sequencing also revealed highly significant structuring in the relative occurrence of carbohydrate-active enzymes (CAZymes). CAZymes were enriched in the rumen relative to the small and large intestines. Interestingly, taxonomic diversity was highest in the large intestine, suggesting an important and understudied role for this organ. Despite the two study populations being separated by an ocean and six millennia of evolutionary history, gut microbiome structuring was remarkably consistent. Our study suggests a strong selection for gut microbiome biogeography along the gastrointestinal tract in reindeer subspecies. |
format |
Article in Journal/Newspaper |
author |
Kamenova, Stefaniya de Muinck, Eric Veiberg, Vebjørn Utsi, Tove Hilde Ågnes Steyaert, Sam Albon, Steve Loe, Leif Egil Trosvik, Pål |
spellingShingle |
Kamenova, Stefaniya de Muinck, Eric Veiberg, Vebjørn Utsi, Tove Hilde Ågnes Steyaert, Sam Albon, Steve Loe, Leif Egil Trosvik, Pål Gut microbiome biogeography in reindeer supersedes millennia of ecological and evolutionary separation |
author_facet |
Kamenova, Stefaniya de Muinck, Eric Veiberg, Vebjørn Utsi, Tove Hilde Ågnes Steyaert, Sam Albon, Steve Loe, Leif Egil Trosvik, Pål |
author_sort |
Kamenova, Stefaniya |
title |
Gut microbiome biogeography in reindeer supersedes millennia of ecological and evolutionary separation |
title_short |
Gut microbiome biogeography in reindeer supersedes millennia of ecological and evolutionary separation |
title_full |
Gut microbiome biogeography in reindeer supersedes millennia of ecological and evolutionary separation |
title_fullStr |
Gut microbiome biogeography in reindeer supersedes millennia of ecological and evolutionary separation |
title_full_unstemmed |
Gut microbiome biogeography in reindeer supersedes millennia of ecological and evolutionary separation |
title_sort |
gut microbiome biogeography in reindeer supersedes millennia of ecological and evolutionary separation |
publisher |
Oxford University Press |
publishDate |
2023 |
url |
https://hdl.handle.net/10037/32290 https://doi.org/10.1093/femsec/fiad157 |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
FEMS Microbiology Ecology info:eu-repo/grantAgreement/EC/H2020/869471/EU/Drivers and Feedbacks of Changes in Arctic Terrestrial Biodiversity/CHARTER/ Kamenova S, de Muinck E, Veiberg V, Utsi TA, Steyaert SM, Albon S, Loe LE, Trosvik P. Gut microbiome biogeography in reindeer supersedes millennia of ecological and evolutionary separation. FEMS Microbiology Ecology. 2023;99(12) FRIDAID 2209851 doi:10.1093/femsec/fiad157 0168-6496 1574-6941 https://hdl.handle.net/10037/32290 |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) openAccess Copyright 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0 |
op_doi |
https://doi.org/10.1093/femsec/fiad157 |
container_title |
FEMS Microbiology Ecology |
container_volume |
99 |
container_issue |
12 |
_version_ |
1789327816349712384 |