Modulation of intestinal growth and differentiation by photoperiod and dietary treatment during smoltification in Atlantic salmon (Salmo salar, L.)
Atlantic salmon undergo smoltification, a process that prepares the fish to enter and thrive in seawater (SW). Several physiological changes occur during smolting, especially in osmoregulatory tissues, the gill, the kidney, and the intestine. Here we characterized the effects on intestinal morphogen...
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Online Access: | https://hdl.handle.net/10037/31716 https://doi.org/10.1016/j.aquaculture.2022.739164 |
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ftunivtroemsoe:oai:munin.uit.no:10037/31716 2023-12-10T09:46:50+01:00 Modulation of intestinal growth and differentiation by photoperiod and dietary treatment during smoltification in Atlantic salmon (Salmo salar, L.) Duarte, Vilma Gaetano, Pasqualina Striberny, Anja Hazlerigg, David Jørgensen, Even Hjalmar Fuentes, Juan Campinho, Marco A. 2022-12-15 https://hdl.handle.net/10037/31716 https://doi.org/10.1016/j.aquaculture.2022.739164 eng eng Elsevier Aquaculture Duarte, Gaetano, Striberny, Hazlerigg, Jørgensen E H, Fuentes, Campinho. Modulation of intestinal growth and differentiation by photoperiod and dietary treatment during smoltification in Atlantic salmon (Salmo salar, L.). Aquaculture. 2023;566 FRIDAID 2134500 doi:10.1016/j.aquaculture.2022.739164 0044-8486 1873-5622 https://hdl.handle.net/10037/31716 openAccess Copyright 2023 The Author(s) Journal article Tidsskriftartikkel Peer reviewed acceptedVersion 2022 ftunivtroemsoe https://doi.org/10.1016/j.aquaculture.2022.739164 2023-11-16T00:08:06Z Atlantic salmon undergo smoltification, a process that prepares the fish to enter and thrive in seawater (SW). Several physiological changes occur during smolting, especially in osmoregulatory tissues, the gill, the kidney, and the intestine. Here we characterized the effects on intestinal morphogenesis of two different, commonly used smoltification regimes during the end of the freshwater phase, photoperiod and/or the addition of salt and amino acid supplements in the diet. We focused on intestinal morphological differentiation, i.e., external perimeter, absorptive perimeter, tissue thickness, and villi density. In addition, we quantified cell proliferation (PCNA positive) and Na + , K + -ATPase (NKA) and Na + , K + ,2Cl − (NKCCs) co-transporters expression and enterocyte apicobasal distribution by immunohistochemistry. These analyses show that the anterior and posterior intestines have different developmental dynamics during smoltification. In both intestinal regions, photoperiod and dietary treatment increased the absorptive perimeter. In addition, diet and photoperiod treatments differentially stimulated NKA protein expression in the anterior intestine. NKCC apical-basolateral expression in the enterocytes increased after SW entry in the anterior and posterior intestines. In conclusion, our results show that, as smoltification progresses, the anterior intestine responds more readily to experimental conditions than the posterior intestine. In our study, photoperiod and dietary treatment seem to enhance the development of the capacity to tolerate SW. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Tromsø: Munin Open Research Archive Aquaculture 566 739164 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
description |
Atlantic salmon undergo smoltification, a process that prepares the fish to enter and thrive in seawater (SW). Several physiological changes occur during smolting, especially in osmoregulatory tissues, the gill, the kidney, and the intestine. Here we characterized the effects on intestinal morphogenesis of two different, commonly used smoltification regimes during the end of the freshwater phase, photoperiod and/or the addition of salt and amino acid supplements in the diet. We focused on intestinal morphological differentiation, i.e., external perimeter, absorptive perimeter, tissue thickness, and villi density. In addition, we quantified cell proliferation (PCNA positive) and Na + , K + -ATPase (NKA) and Na + , K + ,2Cl − (NKCCs) co-transporters expression and enterocyte apicobasal distribution by immunohistochemistry. These analyses show that the anterior and posterior intestines have different developmental dynamics during smoltification. In both intestinal regions, photoperiod and dietary treatment increased the absorptive perimeter. In addition, diet and photoperiod treatments differentially stimulated NKA protein expression in the anterior intestine. NKCC apical-basolateral expression in the enterocytes increased after SW entry in the anterior and posterior intestines. In conclusion, our results show that, as smoltification progresses, the anterior intestine responds more readily to experimental conditions than the posterior intestine. In our study, photoperiod and dietary treatment seem to enhance the development of the capacity to tolerate SW. |
format |
Article in Journal/Newspaper |
author |
Duarte, Vilma Gaetano, Pasqualina Striberny, Anja Hazlerigg, David Jørgensen, Even Hjalmar Fuentes, Juan Campinho, Marco A. |
spellingShingle |
Duarte, Vilma Gaetano, Pasqualina Striberny, Anja Hazlerigg, David Jørgensen, Even Hjalmar Fuentes, Juan Campinho, Marco A. Modulation of intestinal growth and differentiation by photoperiod and dietary treatment during smoltification in Atlantic salmon (Salmo salar, L.) |
author_facet |
Duarte, Vilma Gaetano, Pasqualina Striberny, Anja Hazlerigg, David Jørgensen, Even Hjalmar Fuentes, Juan Campinho, Marco A. |
author_sort |
Duarte, Vilma |
title |
Modulation of intestinal growth and differentiation by photoperiod and dietary treatment during smoltification in Atlantic salmon (Salmo salar, L.) |
title_short |
Modulation of intestinal growth and differentiation by photoperiod and dietary treatment during smoltification in Atlantic salmon (Salmo salar, L.) |
title_full |
Modulation of intestinal growth and differentiation by photoperiod and dietary treatment during smoltification in Atlantic salmon (Salmo salar, L.) |
title_fullStr |
Modulation of intestinal growth and differentiation by photoperiod and dietary treatment during smoltification in Atlantic salmon (Salmo salar, L.) |
title_full_unstemmed |
Modulation of intestinal growth and differentiation by photoperiod and dietary treatment during smoltification in Atlantic salmon (Salmo salar, L.) |
title_sort |
modulation of intestinal growth and differentiation by photoperiod and dietary treatment during smoltification in atlantic salmon (salmo salar, l.) |
publisher |
Elsevier |
publishDate |
2022 |
url |
https://hdl.handle.net/10037/31716 https://doi.org/10.1016/j.aquaculture.2022.739164 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Aquaculture Duarte, Gaetano, Striberny, Hazlerigg, Jørgensen E H, Fuentes, Campinho. Modulation of intestinal growth and differentiation by photoperiod and dietary treatment during smoltification in Atlantic salmon (Salmo salar, L.). Aquaculture. 2023;566 FRIDAID 2134500 doi:10.1016/j.aquaculture.2022.739164 0044-8486 1873-5622 https://hdl.handle.net/10037/31716 |
op_rights |
openAccess Copyright 2023 The Author(s) |
op_doi |
https://doi.org/10.1016/j.aquaculture.2022.739164 |
container_title |
Aquaculture |
container_volume |
566 |
container_start_page |
739164 |
_version_ |
1784890359673782272 |