Photoperiod and dietary treatment in freshwater modulate the short-term intestinal response to seawater in Atlantic salmon (Salmo salar)
Stimulation and timing of smoltification are essential for successful Atlantic salmon (Salmo salar) aquaculture. This study investigated intestinal responses during dietary and photoperiod manipulation in freshwater (FW) and after a subsequent seven days residence in seawater (SW). “Small” and “larg...
Published in: | Aquaculture |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Elsevier
2023
|
Subjects: | |
Online Access: | https://hdl.handle.net/10037/30106 https://doi.org/10.1016/j.aquaculture.2023.739316 |
id |
ftunivtroemsoe:oai:munin.uit.no:10037/30106 |
---|---|
record_format |
openpolar |
spelling |
ftunivtroemsoe:oai:munin.uit.no:10037/30106 2023-09-05T13:18:10+02:00 Photoperiod and dietary treatment in freshwater modulate the short-term intestinal response to seawater in Atlantic salmon (Salmo salar) Gaetano, Pasqualina Duarte, Vilma Striberny, Anja Hazlerigg, David Jørgensen, Even Hjalmar Campinho, Marco A. Fuentes, Juan 2023-02-02 https://hdl.handle.net/10037/30106 https://doi.org/10.1016/j.aquaculture.2023.739316 eng eng Elsevier Aquaculture Gaetano, Duarte, Striberny, Hazlerigg, Jørgensen, Campinho, Fuentes. Photoperiod and dietary treatment in freshwater modulate the short-term intestinal response to seawater in Atlantic salmon (Salmo salar). Aquaculture. 2023;568 FRIDAID 2129200 doi:10.1016/j.aquaculture.2023.739316 0044-8486 1873-5622 https://hdl.handle.net/10037/30106 Attribution 4.0 International (CC BY 4.0) openAccess Copyright 2023 The Author(s) https://creativecommons.org/licenses/by/4.0 Journal article Tidsskriftartikkel Peer reviewed publishedVersion 2023 ftunivtroemsoe https://doi.org/10.1016/j.aquaculture.2023.739316 2023-08-23T23:07:11Z Stimulation and timing of smoltification are essential for successful Atlantic salmon (Salmo salar) aquaculture. This study investigated intestinal responses during dietary and photoperiod manipulation in freshwater (FW) and after a subsequent seven days residence in seawater (SW). “Small” and “large” Atlantic salmon parr (~40 g and ~ 130 g respectively) were treated in FW for 12 weeks and thereafter transferred to SW for seven days. During the FW phase, fish underwent two different light conditions, 24 L:0D – 24 L (“LL-LL” groups) and 7 L:17D – 24 L (“SP-LL” groups) or fed with either regular feed (“LL-LL C" and “SP-LL C" groups) or feed enriched with a salt mix plus free tryptophan (“LL-LL + diet” and “SP-LL + diet” groups). We analyzed Na + /K + -ATPase (NKA) activity, tissue bioelectrical properties in Ussing chambers, and intestinal fluid composition. The NKA activity showed minor variations in relation to fish size, treatments, or intestinal region (anterior or posterior). Photoperiod modulated epithelial bioelectrical properties (I sc and Rt ) of the anterior and posterior intestine, particularly transepithelial resistance (R t ). Pharmacological experiments, targeting apical Na + /K + /2Cl − (NKCC2) and Na + / Cl − (NCC) co-transporters revealed intestinal region- and water salinity-dependent effects. In addition, stimulation of the intracellular cAMP with forskolin and IBMX showed intestinal region-, water salinity, and treatmentdependence responses with clear functional specialization of the anterior and posterior intestine. The intestinal fluid composition reflected the ability to process ingested SW and showed little variation in large fish. In summary, our data suggest a better pre-adaptation of the intestine during light-stimulated smoltification (SP-LL groups), and the combination of light and diet might give, in an industrial aquaculture setting, an advantage to smaller, but not larger smolts. Intestinal fluid composition in small fish can be used as an index of intestinal function and may act as ... Article in Journal/Newspaper Atlantic salmon Salmo salar University of Tromsø: Munin Open Research Archive Aquaculture 568 739316 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
description |
Stimulation and timing of smoltification are essential for successful Atlantic salmon (Salmo salar) aquaculture. This study investigated intestinal responses during dietary and photoperiod manipulation in freshwater (FW) and after a subsequent seven days residence in seawater (SW). “Small” and “large” Atlantic salmon parr (~40 g and ~ 130 g respectively) were treated in FW for 12 weeks and thereafter transferred to SW for seven days. During the FW phase, fish underwent two different light conditions, 24 L:0D – 24 L (“LL-LL” groups) and 7 L:17D – 24 L (“SP-LL” groups) or fed with either regular feed (“LL-LL C" and “SP-LL C" groups) or feed enriched with a salt mix plus free tryptophan (“LL-LL + diet” and “SP-LL + diet” groups). We analyzed Na + /K + -ATPase (NKA) activity, tissue bioelectrical properties in Ussing chambers, and intestinal fluid composition. The NKA activity showed minor variations in relation to fish size, treatments, or intestinal region (anterior or posterior). Photoperiod modulated epithelial bioelectrical properties (I sc and Rt ) of the anterior and posterior intestine, particularly transepithelial resistance (R t ). Pharmacological experiments, targeting apical Na + /K + /2Cl − (NKCC2) and Na + / Cl − (NCC) co-transporters revealed intestinal region- and water salinity-dependent effects. In addition, stimulation of the intracellular cAMP with forskolin and IBMX showed intestinal region-, water salinity, and treatmentdependence responses with clear functional specialization of the anterior and posterior intestine. The intestinal fluid composition reflected the ability to process ingested SW and showed little variation in large fish. In summary, our data suggest a better pre-adaptation of the intestine during light-stimulated smoltification (SP-LL groups), and the combination of light and diet might give, in an industrial aquaculture setting, an advantage to smaller, but not larger smolts. Intestinal fluid composition in small fish can be used as an index of intestinal function and may act as ... |
format |
Article in Journal/Newspaper |
author |
Gaetano, Pasqualina Duarte, Vilma Striberny, Anja Hazlerigg, David Jørgensen, Even Hjalmar Campinho, Marco A. Fuentes, Juan |
spellingShingle |
Gaetano, Pasqualina Duarte, Vilma Striberny, Anja Hazlerigg, David Jørgensen, Even Hjalmar Campinho, Marco A. Fuentes, Juan Photoperiod and dietary treatment in freshwater modulate the short-term intestinal response to seawater in Atlantic salmon (Salmo salar) |
author_facet |
Gaetano, Pasqualina Duarte, Vilma Striberny, Anja Hazlerigg, David Jørgensen, Even Hjalmar Campinho, Marco A. Fuentes, Juan |
author_sort |
Gaetano, Pasqualina |
title |
Photoperiod and dietary treatment in freshwater modulate the short-term intestinal response to seawater in Atlantic salmon (Salmo salar) |
title_short |
Photoperiod and dietary treatment in freshwater modulate the short-term intestinal response to seawater in Atlantic salmon (Salmo salar) |
title_full |
Photoperiod and dietary treatment in freshwater modulate the short-term intestinal response to seawater in Atlantic salmon (Salmo salar) |
title_fullStr |
Photoperiod and dietary treatment in freshwater modulate the short-term intestinal response to seawater in Atlantic salmon (Salmo salar) |
title_full_unstemmed |
Photoperiod and dietary treatment in freshwater modulate the short-term intestinal response to seawater in Atlantic salmon (Salmo salar) |
title_sort |
photoperiod and dietary treatment in freshwater modulate the short-term intestinal response to seawater in atlantic salmon (salmo salar) |
publisher |
Elsevier |
publishDate |
2023 |
url |
https://hdl.handle.net/10037/30106 https://doi.org/10.1016/j.aquaculture.2023.739316 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Aquaculture Gaetano, Duarte, Striberny, Hazlerigg, Jørgensen, Campinho, Fuentes. Photoperiod and dietary treatment in freshwater modulate the short-term intestinal response to seawater in Atlantic salmon (Salmo salar). Aquaculture. 2023;568 FRIDAID 2129200 doi:10.1016/j.aquaculture.2023.739316 0044-8486 1873-5622 https://hdl.handle.net/10037/30106 |
op_rights |
Attribution 4.0 International (CC BY 4.0) openAccess Copyright 2023 The Author(s) https://creativecommons.org/licenses/by/4.0 |
op_doi |
https://doi.org/10.1016/j.aquaculture.2023.739316 |
container_title |
Aquaculture |
container_volume |
568 |
container_start_page |
739316 |
_version_ |
1776199200224051200 |