Assessing new prognostic biomarkers in resected colon cancer patients
Colon cancer is a common malignancy. We retrospectively established a biobank including 452 patients operated for colon cancer stage I-III in Northern Norway in the period 1998-2007. Tissue microarrays (TMAs) containing tumor tissue from these patients were constructed, and subsequently biomarker ex...
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ftunivtroemsoe:oai:munin.uit.no:10037/29356 2023-06-18T03:42:17+02:00 Assessing new prognostic biomarkers in resected colon cancer patients Selven, Hallgeir 2023-06-22 https://hdl.handle.net/10037/29356 eng eng UiT The Arctic University of Norway UiT Norges arktiske universitet Paper I: Selven, H., Busund, L.R., Andersen, S., Bremnes, R.M. & Kilvær, T.K. (2021). High expression of microRNA-126 relates to favorable prognosis for colon cancer patients. Scientific Reports, 11 , 9592. Also available in Munin at https://hdl.handle.net/10037/21662 . Paper II: Selven, H., Andersen, S., Pedersen, M.I., Lombardi, A.P.G., Busund, L.R. & Kilvær, T.K. (2022). High expression of miR-17-5p and miR-20a-5p predicts favorable disease-specific survival in stage I-III colon cancer. Scientific Reports, 12 , 7080. Also available in Munin at https://hdl.handle.net/10037/26661 . Paper III: Selven, H., Busund, L.R., Pedersen, M.I., Lombardi, A.P.G., Andersen, S. & Kilvær, T.K. (2023). High expression of IRS-1, RUNX3 and SMAD4 are positive prognostic factors in stage I-III colon cancer. Cancers, 15 (5), 1448. Also available at https://doi.org/10.3390/cancers15051448 . https://hdl.handle.net/10037/29356 openAccess Copyright 2023 The Author(s) VDP::Medical disciplines: 700::Clinical medical disciplines: 750::Oncology: 762 VDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Onkologi: 762 DOKTOR-003 Doctoral thesis Doktorgradsavhandling 2023 ftunivtroemsoe 2023-06-07T23:06:22Z Colon cancer is a common malignancy. We retrospectively established a biobank including 452 patients operated for colon cancer stage I-III in Northern Norway in the period 1998-2007. Tissue microarrays (TMAs) containing tumor tissue from these patients were constructed, and subsequently biomarker expression assessed by digital pathology. By using in situ hybridization, we assessed the expression of miR-126, miR-17-5p and miR-20a-5p. A high expression of all these miRs was related to improved disease-specific survival for these patients. For miR-17-5p and miR-20a-5p we investigated their functional aspects in select colon cancer cell lines. Over-expression of miR-17-5p and miR-20a-5p did not impact viability or invasion, and mitigated migration, strengthening our results of improved survival upon high expression. Additionally, using immunohistochemistry, we wanted to investigate four proteins thought to be regulated by these miRs; IRS-1, IRS-2, RUNX3 and SMAD4. In our material, a high expression of IRS-1, RUNX3 and SMAD4 was related to a favorable survival. These novel biomarkers could improve risk stratification in colon cancer patients, differentiating patients with a high risk of recurrence vs patients with a low risk of recurrence in the same TNM-stage. This could help the oncologists to choose the appropriate adjuvant treatment. For this to be implemented in clinical practice, the results need to be verified in large prospective trials. Doctoral or Postdoctoral Thesis Northern Norway University of Tromsø: Munin Open Research Archive Norway TNM ENVELOPE(-58.100,-58.100,-62.000,-62.000) |
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University of Tromsø: Munin Open Research Archive |
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ftunivtroemsoe |
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English |
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VDP::Medical disciplines: 700::Clinical medical disciplines: 750::Oncology: 762 VDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Onkologi: 762 DOKTOR-003 |
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VDP::Medical disciplines: 700::Clinical medical disciplines: 750::Oncology: 762 VDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Onkologi: 762 DOKTOR-003 Selven, Hallgeir Assessing new prognostic biomarkers in resected colon cancer patients |
topic_facet |
VDP::Medical disciplines: 700::Clinical medical disciplines: 750::Oncology: 762 VDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Onkologi: 762 DOKTOR-003 |
description |
Colon cancer is a common malignancy. We retrospectively established a biobank including 452 patients operated for colon cancer stage I-III in Northern Norway in the period 1998-2007. Tissue microarrays (TMAs) containing tumor tissue from these patients were constructed, and subsequently biomarker expression assessed by digital pathology. By using in situ hybridization, we assessed the expression of miR-126, miR-17-5p and miR-20a-5p. A high expression of all these miRs was related to improved disease-specific survival for these patients. For miR-17-5p and miR-20a-5p we investigated their functional aspects in select colon cancer cell lines. Over-expression of miR-17-5p and miR-20a-5p did not impact viability or invasion, and mitigated migration, strengthening our results of improved survival upon high expression. Additionally, using immunohistochemistry, we wanted to investigate four proteins thought to be regulated by these miRs; IRS-1, IRS-2, RUNX3 and SMAD4. In our material, a high expression of IRS-1, RUNX3 and SMAD4 was related to a favorable survival. These novel biomarkers could improve risk stratification in colon cancer patients, differentiating patients with a high risk of recurrence vs patients with a low risk of recurrence in the same TNM-stage. This could help the oncologists to choose the appropriate adjuvant treatment. For this to be implemented in clinical practice, the results need to be verified in large prospective trials. |
format |
Doctoral or Postdoctoral Thesis |
author |
Selven, Hallgeir |
author_facet |
Selven, Hallgeir |
author_sort |
Selven, Hallgeir |
title |
Assessing new prognostic biomarkers in resected colon cancer patients |
title_short |
Assessing new prognostic biomarkers in resected colon cancer patients |
title_full |
Assessing new prognostic biomarkers in resected colon cancer patients |
title_fullStr |
Assessing new prognostic biomarkers in resected colon cancer patients |
title_full_unstemmed |
Assessing new prognostic biomarkers in resected colon cancer patients |
title_sort |
assessing new prognostic biomarkers in resected colon cancer patients |
publisher |
UiT The Arctic University of Norway |
publishDate |
2023 |
url |
https://hdl.handle.net/10037/29356 |
long_lat |
ENVELOPE(-58.100,-58.100,-62.000,-62.000) |
geographic |
Norway TNM |
geographic_facet |
Norway TNM |
genre |
Northern Norway |
genre_facet |
Northern Norway |
op_relation |
Paper I: Selven, H., Busund, L.R., Andersen, S., Bremnes, R.M. & Kilvær, T.K. (2021). High expression of microRNA-126 relates to favorable prognosis for colon cancer patients. Scientific Reports, 11 , 9592. Also available in Munin at https://hdl.handle.net/10037/21662 . Paper II: Selven, H., Andersen, S., Pedersen, M.I., Lombardi, A.P.G., Busund, L.R. & Kilvær, T.K. (2022). High expression of miR-17-5p and miR-20a-5p predicts favorable disease-specific survival in stage I-III colon cancer. Scientific Reports, 12 , 7080. Also available in Munin at https://hdl.handle.net/10037/26661 . Paper III: Selven, H., Busund, L.R., Pedersen, M.I., Lombardi, A.P.G., Andersen, S. & Kilvær, T.K. (2023). High expression of IRS-1, RUNX3 and SMAD4 are positive prognostic factors in stage I-III colon cancer. Cancers, 15 (5), 1448. Also available at https://doi.org/10.3390/cancers15051448 . https://hdl.handle.net/10037/29356 |
op_rights |
openAccess Copyright 2023 The Author(s) |
_version_ |
1769008174361214976 |