Genomic Sequencing and Phylogenomics of Cowpox Virus
Cowpox virus (CPXV; genus Orthopoxvirus family Poxviridae ) is the causative agent of cowpox, a self-limiting zoonotic infection. CPXV is endemic in Eurasia, and human CPXV infections are associated with exposure to infected animals. In the Fennoscandian region, five CPXVs isolated from cats and hum...
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ftunivtroemsoe:oai:munin.uit.no:10037/27909 2023-06-11T04:11:40+02:00 Genomic Sequencing and Phylogenomics of Cowpox Virus Diaz Cánova, Diana Karina Mavian, Carla Brinkmann, Annika Nitsche, Andreas Moens, Ugo Okeke, Malachy Ifeanyi 2022-09-28 https://hdl.handle.net/10037/27909 https://doi.org/10.3390/v14102134 eng eng MDPI Diaz-Cánova, D. (2023). Evolutionary genomics of cowpox virus and recombination in vitro between a naturally occurring cowpox virus and a vaccinia virus vectored influenza vaccine. (Doctoral thesis). https://hdl.handle.net/10037/29180 . Viruses Diaz Cánova, Mavian, Brinkmann, Nitsche, Moens, Okeke. Genomic Sequencing and Phylogenomics of Cowpox Virus. Viruses. 2022;14(10) FRIDAID 2083386 doi:10.3390/v14102134 1999-4915 https://hdl.handle.net/10037/27909 Attribution 4.0 International (CC BY 4.0) openAccess Copyright 2022 The Author(s) https://creativecommons.org/licenses/by/4.0 Journal article Tidsskriftartikkel Peer reviewed publishedVersion 2022 ftunivtroemsoe https://doi.org/10.3390/v14102134 2023-05-17T23:06:11Z Cowpox virus (CPXV; genus Orthopoxvirus family Poxviridae ) is the causative agent of cowpox, a self-limiting zoonotic infection. CPXV is endemic in Eurasia, and human CPXV infections are associated with exposure to infected animals. In the Fennoscandian region, five CPXVs isolated from cats and humans were collected and used in this study. We report the complete sequence of their genomes, which ranged in size from 220–222 kbp, containing between 215 and 219 open reading frames. The phylogenetic analysis of 87 orthopoxvirus strains, including the Fennoscandian CPXV isolates, confirmed the division of CPXV strains into at least five distinct major clusters (CPXV-like 1, CPXV-like 2, VACV-like, VARV-like and ECTV-Abatino-like) and can be further divided into eighteen sub-species based on the genetic and patristic distances. Bayesian time-scaled evolutionary history of CPXV was reconstructed employing concatenated 62 non-recombinant conserved genes of 55 CPXV. The CPXV evolution rate was calculated to be 1.65 × 10 −5 substitution/site/year. Our findings confirmed that CPXV is not a single species but a polyphyletic assemblage of several species and thus, a reclassification is warranted. Article in Journal/Newspaper Fennoscandian University of Tromsø: Munin Open Research Archive Viruses 14 10 2134 |
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University of Tromsø: Munin Open Research Archive |
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ftunivtroemsoe |
language |
English |
description |
Cowpox virus (CPXV; genus Orthopoxvirus family Poxviridae ) is the causative agent of cowpox, a self-limiting zoonotic infection. CPXV is endemic in Eurasia, and human CPXV infections are associated with exposure to infected animals. In the Fennoscandian region, five CPXVs isolated from cats and humans were collected and used in this study. We report the complete sequence of their genomes, which ranged in size from 220–222 kbp, containing between 215 and 219 open reading frames. The phylogenetic analysis of 87 orthopoxvirus strains, including the Fennoscandian CPXV isolates, confirmed the division of CPXV strains into at least five distinct major clusters (CPXV-like 1, CPXV-like 2, VACV-like, VARV-like and ECTV-Abatino-like) and can be further divided into eighteen sub-species based on the genetic and patristic distances. Bayesian time-scaled evolutionary history of CPXV was reconstructed employing concatenated 62 non-recombinant conserved genes of 55 CPXV. The CPXV evolution rate was calculated to be 1.65 × 10 −5 substitution/site/year. Our findings confirmed that CPXV is not a single species but a polyphyletic assemblage of several species and thus, a reclassification is warranted. |
format |
Article in Journal/Newspaper |
author |
Diaz Cánova, Diana Karina Mavian, Carla Brinkmann, Annika Nitsche, Andreas Moens, Ugo Okeke, Malachy Ifeanyi |
spellingShingle |
Diaz Cánova, Diana Karina Mavian, Carla Brinkmann, Annika Nitsche, Andreas Moens, Ugo Okeke, Malachy Ifeanyi Genomic Sequencing and Phylogenomics of Cowpox Virus |
author_facet |
Diaz Cánova, Diana Karina Mavian, Carla Brinkmann, Annika Nitsche, Andreas Moens, Ugo Okeke, Malachy Ifeanyi |
author_sort |
Diaz Cánova, Diana Karina |
title |
Genomic Sequencing and Phylogenomics of Cowpox Virus |
title_short |
Genomic Sequencing and Phylogenomics of Cowpox Virus |
title_full |
Genomic Sequencing and Phylogenomics of Cowpox Virus |
title_fullStr |
Genomic Sequencing and Phylogenomics of Cowpox Virus |
title_full_unstemmed |
Genomic Sequencing and Phylogenomics of Cowpox Virus |
title_sort |
genomic sequencing and phylogenomics of cowpox virus |
publisher |
MDPI |
publishDate |
2022 |
url |
https://hdl.handle.net/10037/27909 https://doi.org/10.3390/v14102134 |
genre |
Fennoscandian |
genre_facet |
Fennoscandian |
op_relation |
Diaz-Cánova, D. (2023). Evolutionary genomics of cowpox virus and recombination in vitro between a naturally occurring cowpox virus and a vaccinia virus vectored influenza vaccine. (Doctoral thesis). https://hdl.handle.net/10037/29180 . Viruses Diaz Cánova, Mavian, Brinkmann, Nitsche, Moens, Okeke. Genomic Sequencing and Phylogenomics of Cowpox Virus. Viruses. 2022;14(10) FRIDAID 2083386 doi:10.3390/v14102134 1999-4915 https://hdl.handle.net/10037/27909 |
op_rights |
Attribution 4.0 International (CC BY 4.0) openAccess Copyright 2022 The Author(s) https://creativecommons.org/licenses/by/4.0 |
op_doi |
https://doi.org/10.3390/v14102134 |
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Viruses |
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14 |
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10 |
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2134 |
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1768386883347283968 |