Chitinolytic enzymes contribute to the pathogenicity of Aliivibrio salmonicida LFI1238 in the invasive phase of cold-water vibriosis
Background - Aliivibrio salmonicida is the causative agent of cold-water vibriosis in salmonids (Oncorhynchus mykiss and Salmo salar L.) and gadidae (Gadus morhua L.). Virulence-associated factors that are essential for the full spectrum of A. salmonicida pathogenicity are largely unknown. Chitin-ac...
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ftunivtroemsoe:oai:munin.uit.no:10037/27483 2023-05-15T15:31:33+02:00 Chitinolytic enzymes contribute to the pathogenicity of Aliivibrio salmonicida LFI1238 in the invasive phase of cold-water vibriosis Skåne, Anna Edvardsen, Per Kristian Thorén Cordara, Gabriele Loose, Jennifer Sarah Maria Leitl, Kira Daryl Krengel, Ute Sørum, Henning Askarian, Fatemeh Vaaje-Kolstad, Gustav 2022-08-08 https://hdl.handle.net/10037/27483 https://doi.org/10.1186/s12866-022-02590-2 eng eng BMC BMC Microbiology info:eu-repo/grantAgreement/EC/EXCELLENT SCIENCE/730872/Norway/Convenient Access to Light Sources Open to Innovation, Science and to the World/CALIPSOplus/ Skåne, Edvardsen, Cordara, Loose, Leitl, Krengel, Sørum, Askarian, Vaaje-Kolstad. Chitinolytic enzymes contribute to the pathogenicity of Aliivibrio salmonicida LFI1238 in the invasive phase of cold-water vibriosis. BMC Microbiology. 2022;22(1) FRIDAID 2064442 doi:10.1186/s12866-022-02590-2 1471-2180 https://hdl.handle.net/10037/27483 Attribution 4.0 International (CC BY 4.0) openAccess Copyright 2022 The Author(s) https://creativecommons.org/licenses/by/4.0 CC-BY Journal article Tidsskriftartikkel Peer reviewed publishedVersion 2022 ftunivtroemsoe https://doi.org/10.1186/s12866-022-02590-2 2022-11-24T00:02:11Z Background - Aliivibrio salmonicida is the causative agent of cold-water vibriosis in salmonids (Oncorhynchus mykiss and Salmo salar L.) and gadidae (Gadus morhua L.). Virulence-associated factors that are essential for the full spectrum of A. salmonicida pathogenicity are largely unknown. Chitin-active lytic polysaccharide monooxygenases (LPMOs) have been indicated to play roles in both chitin degradation and virulence in a variety of pathogenic bacteria but are largely unexplored in this context. Results - In the present study we investigated the role of LPMOs in the pathogenicity of A. salmonicida LFI238 in Atlantic salmon (Salmo salar L.). In vivo challenge experiments using isogenic deletion mutants of the two LPMOs encoding genes AsLPMO10A and AsLPMO10B, showed that both LPMOs, and in particular AsLPMO10B, were important in the invasive phase of cold-water vibriosis. Crystallographic analysis of the AsLPMO10B AA10 LPMO domain (to 1.4 Å resolution) revealed high structural similarity to viral fusolin, an LPMO known to enhance the virulence of insecticidal agents. Finally, exposure to Atlantic salmon serum resulted in substantial proteome re-organization of the A. salmonicida LPMO deletion variants compared to the wild type strain, indicating the struggle of the bacterium to adapt to the host immune components in the absence of the LPMOs. Conclusion - The present study consolidates the role of LPMOs in virulence and demonstrates that such enzymes may have more than one function. Article in Journal/Newspaper Atlantic salmon Gadus morhua Salmo salar University of Tromsø: Munin Open Research Archive BMC Microbiology 22 1 |
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Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
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ftunivtroemsoe |
language |
English |
description |
Background - Aliivibrio salmonicida is the causative agent of cold-water vibriosis in salmonids (Oncorhynchus mykiss and Salmo salar L.) and gadidae (Gadus morhua L.). Virulence-associated factors that are essential for the full spectrum of A. salmonicida pathogenicity are largely unknown. Chitin-active lytic polysaccharide monooxygenases (LPMOs) have been indicated to play roles in both chitin degradation and virulence in a variety of pathogenic bacteria but are largely unexplored in this context. Results - In the present study we investigated the role of LPMOs in the pathogenicity of A. salmonicida LFI238 in Atlantic salmon (Salmo salar L.). In vivo challenge experiments using isogenic deletion mutants of the two LPMOs encoding genes AsLPMO10A and AsLPMO10B, showed that both LPMOs, and in particular AsLPMO10B, were important in the invasive phase of cold-water vibriosis. Crystallographic analysis of the AsLPMO10B AA10 LPMO domain (to 1.4 Å resolution) revealed high structural similarity to viral fusolin, an LPMO known to enhance the virulence of insecticidal agents. Finally, exposure to Atlantic salmon serum resulted in substantial proteome re-organization of the A. salmonicida LPMO deletion variants compared to the wild type strain, indicating the struggle of the bacterium to adapt to the host immune components in the absence of the LPMOs. Conclusion - The present study consolidates the role of LPMOs in virulence and demonstrates that such enzymes may have more than one function. |
format |
Article in Journal/Newspaper |
author |
Skåne, Anna Edvardsen, Per Kristian Thorén Cordara, Gabriele Loose, Jennifer Sarah Maria Leitl, Kira Daryl Krengel, Ute Sørum, Henning Askarian, Fatemeh Vaaje-Kolstad, Gustav |
spellingShingle |
Skåne, Anna Edvardsen, Per Kristian Thorén Cordara, Gabriele Loose, Jennifer Sarah Maria Leitl, Kira Daryl Krengel, Ute Sørum, Henning Askarian, Fatemeh Vaaje-Kolstad, Gustav Chitinolytic enzymes contribute to the pathogenicity of Aliivibrio salmonicida LFI1238 in the invasive phase of cold-water vibriosis |
author_facet |
Skåne, Anna Edvardsen, Per Kristian Thorén Cordara, Gabriele Loose, Jennifer Sarah Maria Leitl, Kira Daryl Krengel, Ute Sørum, Henning Askarian, Fatemeh Vaaje-Kolstad, Gustav |
author_sort |
Skåne, Anna |
title |
Chitinolytic enzymes contribute to the pathogenicity of Aliivibrio salmonicida LFI1238 in the invasive phase of cold-water vibriosis |
title_short |
Chitinolytic enzymes contribute to the pathogenicity of Aliivibrio salmonicida LFI1238 in the invasive phase of cold-water vibriosis |
title_full |
Chitinolytic enzymes contribute to the pathogenicity of Aliivibrio salmonicida LFI1238 in the invasive phase of cold-water vibriosis |
title_fullStr |
Chitinolytic enzymes contribute to the pathogenicity of Aliivibrio salmonicida LFI1238 in the invasive phase of cold-water vibriosis |
title_full_unstemmed |
Chitinolytic enzymes contribute to the pathogenicity of Aliivibrio salmonicida LFI1238 in the invasive phase of cold-water vibriosis |
title_sort |
chitinolytic enzymes contribute to the pathogenicity of aliivibrio salmonicida lfi1238 in the invasive phase of cold-water vibriosis |
publisher |
BMC |
publishDate |
2022 |
url |
https://hdl.handle.net/10037/27483 https://doi.org/10.1186/s12866-022-02590-2 |
genre |
Atlantic salmon Gadus morhua Salmo salar |
genre_facet |
Atlantic salmon Gadus morhua Salmo salar |
op_relation |
BMC Microbiology info:eu-repo/grantAgreement/EC/EXCELLENT SCIENCE/730872/Norway/Convenient Access to Light Sources Open to Innovation, Science and to the World/CALIPSOplus/ Skåne, Edvardsen, Cordara, Loose, Leitl, Krengel, Sørum, Askarian, Vaaje-Kolstad. Chitinolytic enzymes contribute to the pathogenicity of Aliivibrio salmonicida LFI1238 in the invasive phase of cold-water vibriosis. BMC Microbiology. 2022;22(1) FRIDAID 2064442 doi:10.1186/s12866-022-02590-2 1471-2180 https://hdl.handle.net/10037/27483 |
op_rights |
Attribution 4.0 International (CC BY 4.0) openAccess Copyright 2022 The Author(s) https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1186/s12866-022-02590-2 |
container_title |
BMC Microbiology |
container_volume |
22 |
container_issue |
1 |
_version_ |
1766362071356669952 |