Genomic Sequencing and Analysis of a Novel Human Cowpox Virus With Mosaic Sequences From North America and Old World Orthopoxvirus
Orthopoxviruses (OPXVs) not only infect their natural hosts, but some OPXVs can also cause disease in humans. Previously, we partially characterized an OPXV isolated from an 18-year-old male living in Northern Norway. Restriction enzyme analysis and partial genome sequencing characterized this virus...
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Online Access: | https://hdl.handle.net/10037/27360 https://doi.org/10.3389/fmicb.2022.868887 |
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ftunivtroemsoe:oai:munin.uit.no:10037/27360 2023-06-11T04:15:21+02:00 Genomic Sequencing and Analysis of a Novel Human Cowpox Virus With Mosaic Sequences From North America and Old World Orthopoxvirus Diaz Cánova, Diana Karina Moens, Ugo Brinkmann, Annika Nitsche, Andreas Okeke, malachy ifeanyi 2022-05-03 https://hdl.handle.net/10037/27360 https://doi.org/10.3389/fmicb.2022.868887 eng eng Frontiers Media Diaz-Cánova, D. (2023). Evolutionary genomics of cowpox virus and recombination in vitro between a naturally occurring cowpox virus and a vaccinia virus vectored influenza vaccine. (Doctoral thesis). https://hdl.handle.net/10037/29180 . Frontiers in Microbiology Diaz Cánova, Moens, Brinkmann, Nitsche, Okeke. Genomic Sequencing and Analysis of a Novel Human Cowpox Virus With Mosaic Sequences From North America and Old World Orthopoxvirus. Frontiers in Microbiology. 2022;13 FRIDAID 2045889 doi:10.3389/fmicb.2022.868887 1664-302X https://hdl.handle.net/10037/27360 Attribution 4.0 International (CC BY 4.0) openAccess Copyright 2022 The Author(s) https://creativecommons.org/licenses/by/4.0 Journal article Tidsskriftartikkel Peer reviewed publishedVersion 2022 ftunivtroemsoe https://doi.org/10.3389/fmicb.2022.868887 2023-05-17T23:06:11Z Orthopoxviruses (OPXVs) not only infect their natural hosts, but some OPXVs can also cause disease in humans. Previously, we partially characterized an OPXV isolated from an 18-year-old male living in Northern Norway. Restriction enzyme analysis and partial genome sequencing characterized this virus as an atypical cowpox virus (CPXV), which we named CPXV-No-H2. In this study, we determined the complete genome sequence of CPXV-No-H2 using Illumina and Nanopore sequencing. Our results showed that the whole CPXV-No-H2 genome is 220,276 base pairs (bp) in length, with inverted terminal repeat regions of approximately 7 kbp, containing 217 predicted genes. Seventeen predicted CPXV-No-H2 proteins were most similar to OPXV proteins from the Old World, including Ectromelia virus (ECTV) and Vaccinia virus, and North America, Alaskapox virus (AKPV). CPXV-No-H2 has a mosaic genome with genes most similar to other OPXV genes, and seven potential recombination events were identified. The phylogenetic analysis showed that CPXV-No-H2 formed a separate clade with the German CPXV isolates CPXV_GerMygEK938_17 and CPXV_Ger2010_MKY, sharing 96.4 and 96.3% nucleotide identity, respectively, and this clade clustered closely with the ECTV-OPXV Abatino clade. CPXV-No-H2 is a mosaic virus that may have arisen out of several recombination events between OPXVs, and its phylogenetic clustering suggests that ECTV-Abatino-like cowpox viruses form a distinct, new clade of cowpox viruses. Article in Journal/Newspaper Northern Norway University of Tromsø: Munin Open Research Archive Norway Frontiers in Microbiology 13 |
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University of Tromsø: Munin Open Research Archive |
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English |
description |
Orthopoxviruses (OPXVs) not only infect their natural hosts, but some OPXVs can also cause disease in humans. Previously, we partially characterized an OPXV isolated from an 18-year-old male living in Northern Norway. Restriction enzyme analysis and partial genome sequencing characterized this virus as an atypical cowpox virus (CPXV), which we named CPXV-No-H2. In this study, we determined the complete genome sequence of CPXV-No-H2 using Illumina and Nanopore sequencing. Our results showed that the whole CPXV-No-H2 genome is 220,276 base pairs (bp) in length, with inverted terminal repeat regions of approximately 7 kbp, containing 217 predicted genes. Seventeen predicted CPXV-No-H2 proteins were most similar to OPXV proteins from the Old World, including Ectromelia virus (ECTV) and Vaccinia virus, and North America, Alaskapox virus (AKPV). CPXV-No-H2 has a mosaic genome with genes most similar to other OPXV genes, and seven potential recombination events were identified. The phylogenetic analysis showed that CPXV-No-H2 formed a separate clade with the German CPXV isolates CPXV_GerMygEK938_17 and CPXV_Ger2010_MKY, sharing 96.4 and 96.3% nucleotide identity, respectively, and this clade clustered closely with the ECTV-OPXV Abatino clade. CPXV-No-H2 is a mosaic virus that may have arisen out of several recombination events between OPXVs, and its phylogenetic clustering suggests that ECTV-Abatino-like cowpox viruses form a distinct, new clade of cowpox viruses. |
format |
Article in Journal/Newspaper |
author |
Diaz Cánova, Diana Karina Moens, Ugo Brinkmann, Annika Nitsche, Andreas Okeke, malachy ifeanyi |
spellingShingle |
Diaz Cánova, Diana Karina Moens, Ugo Brinkmann, Annika Nitsche, Andreas Okeke, malachy ifeanyi Genomic Sequencing and Analysis of a Novel Human Cowpox Virus With Mosaic Sequences From North America and Old World Orthopoxvirus |
author_facet |
Diaz Cánova, Diana Karina Moens, Ugo Brinkmann, Annika Nitsche, Andreas Okeke, malachy ifeanyi |
author_sort |
Diaz Cánova, Diana Karina |
title |
Genomic Sequencing and Analysis of a Novel Human Cowpox Virus With Mosaic Sequences From North America and Old World Orthopoxvirus |
title_short |
Genomic Sequencing and Analysis of a Novel Human Cowpox Virus With Mosaic Sequences From North America and Old World Orthopoxvirus |
title_full |
Genomic Sequencing and Analysis of a Novel Human Cowpox Virus With Mosaic Sequences From North America and Old World Orthopoxvirus |
title_fullStr |
Genomic Sequencing and Analysis of a Novel Human Cowpox Virus With Mosaic Sequences From North America and Old World Orthopoxvirus |
title_full_unstemmed |
Genomic Sequencing and Analysis of a Novel Human Cowpox Virus With Mosaic Sequences From North America and Old World Orthopoxvirus |
title_sort |
genomic sequencing and analysis of a novel human cowpox virus with mosaic sequences from north america and old world orthopoxvirus |
publisher |
Frontiers Media |
publishDate |
2022 |
url |
https://hdl.handle.net/10037/27360 https://doi.org/10.3389/fmicb.2022.868887 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Northern Norway |
genre_facet |
Northern Norway |
op_relation |
Diaz-Cánova, D. (2023). Evolutionary genomics of cowpox virus and recombination in vitro between a naturally occurring cowpox virus and a vaccinia virus vectored influenza vaccine. (Doctoral thesis). https://hdl.handle.net/10037/29180 . Frontiers in Microbiology Diaz Cánova, Moens, Brinkmann, Nitsche, Okeke. Genomic Sequencing and Analysis of a Novel Human Cowpox Virus With Mosaic Sequences From North America and Old World Orthopoxvirus. Frontiers in Microbiology. 2022;13 FRIDAID 2045889 doi:10.3389/fmicb.2022.868887 1664-302X https://hdl.handle.net/10037/27360 |
op_rights |
Attribution 4.0 International (CC BY 4.0) openAccess Copyright 2022 The Author(s) https://creativecommons.org/licenses/by/4.0 |
op_doi |
https://doi.org/10.3389/fmicb.2022.868887 |
container_title |
Frontiers in Microbiology |
container_volume |
13 |
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1768372100406444032 |