Evaluation of histological post-mortem changes in farmed Atlantic salmon (Salmo salar L.) at different time intervals and storage temperatures
The aim of this study was to evaluate histologic post-mortem autolytic changes in farmed Atlantic salmon. The fish were either stored at room temperature (RT, 21°C), refrigerated (4°C) or frozen (−20°C), while fish necropsy was performed at 0, 1, 4, 24 and 48 h post-storage (hps). In addition, gills...
Published in: | Journal of Fish Diseases |
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Online Access: | https://hdl.handle.net/10037/27210 https://doi.org/10.1111/jfd.13681 |
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ftunivtroemsoe:oai:munin.uit.no:10037/27210 2023-05-15T15:31:23+02:00 Evaluation of histological post-mortem changes in farmed Atlantic salmon (Salmo salar L.) at different time intervals and storage temperatures Furnesvik, Lisa Erkinharju, Toni Hansen, Miroslava Yousaf, Muhammad Naveed Seternes, Tore 2022-06-29 https://hdl.handle.net/10037/27210 https://doi.org/10.1111/jfd.13681 eng eng Wiley Journal of Fish Diseases Furnesvik, Erkinharju, Hansen, Yousaf, Seternes. Evaluation of histological post-mortem changes in farmed Atlantic salmon (Salmo salar L.) at different time intervals and storage temperatures. Journal of Fish Diseases. 2022;45(10):1571-1580 FRIDAID 2054455 doi:10.1111/jfd.13681 0140-7775 1365-2761 https://hdl.handle.net/10037/27210 Attribution 4.0 International (CC BY 4.0) openAccess Copyright 2022 The Author(s) https://creativecommons.org/licenses/by/4.0 CC-BY Journal article Tidsskriftartikkel Peer reviewed publishedVersion 2022 ftunivtroemsoe https://doi.org/10.1111/jfd.13681 2022-11-03T00:01:18Z The aim of this study was to evaluate histologic post-mortem autolytic changes in farmed Atlantic salmon. The fish were either stored at room temperature (RT, 21°C), refrigerated (4°C) or frozen (−20°C), while fish necropsy was performed at 0, 1, 4, 24 and 48 h post-storage (hps). In addition, gills were sampled at 0, 5, 10, 15, 30 and 45 min post-storage (mps) at room temperature (RT). The haematoxylin and eosin-stained tissue slides were evaluated and scored by using a semi-quantitative scoring system. Our findings demonstrated gills and pyloric caeca/pancreas as the most severely autolysed organs while heart and skeletal musculature were least affected. Generally, moderate to severe autolysis appeared first at 4 hps, while severe changes were seen at 24 hps. Gills demonstrated autolytic changes as early as 10 mps and pyloric caeca/pancreas at 1 hps. Freezing did not prevent the autolysis and even contributed to freezing artefacts, which may lead to misdiagnosis. Keeping organs refrigerated slowed the autolytic progress within the first 4 hps marginally. This study recommends gills and pyloric caeca/pancreas should be sampled as early as possible, at least within 10 min post-necropsy. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Tromsø: Munin Open Research Archive Journal of Fish Diseases 45 10 1571 1580 |
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Open Polar |
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University of Tromsø: Munin Open Research Archive |
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ftunivtroemsoe |
language |
English |
description |
The aim of this study was to evaluate histologic post-mortem autolytic changes in farmed Atlantic salmon. The fish were either stored at room temperature (RT, 21°C), refrigerated (4°C) or frozen (−20°C), while fish necropsy was performed at 0, 1, 4, 24 and 48 h post-storage (hps). In addition, gills were sampled at 0, 5, 10, 15, 30 and 45 min post-storage (mps) at room temperature (RT). The haematoxylin and eosin-stained tissue slides were evaluated and scored by using a semi-quantitative scoring system. Our findings demonstrated gills and pyloric caeca/pancreas as the most severely autolysed organs while heart and skeletal musculature were least affected. Generally, moderate to severe autolysis appeared first at 4 hps, while severe changes were seen at 24 hps. Gills demonstrated autolytic changes as early as 10 mps and pyloric caeca/pancreas at 1 hps. Freezing did not prevent the autolysis and even contributed to freezing artefacts, which may lead to misdiagnosis. Keeping organs refrigerated slowed the autolytic progress within the first 4 hps marginally. This study recommends gills and pyloric caeca/pancreas should be sampled as early as possible, at least within 10 min post-necropsy. |
format |
Article in Journal/Newspaper |
author |
Furnesvik, Lisa Erkinharju, Toni Hansen, Miroslava Yousaf, Muhammad Naveed Seternes, Tore |
spellingShingle |
Furnesvik, Lisa Erkinharju, Toni Hansen, Miroslava Yousaf, Muhammad Naveed Seternes, Tore Evaluation of histological post-mortem changes in farmed Atlantic salmon (Salmo salar L.) at different time intervals and storage temperatures |
author_facet |
Furnesvik, Lisa Erkinharju, Toni Hansen, Miroslava Yousaf, Muhammad Naveed Seternes, Tore |
author_sort |
Furnesvik, Lisa |
title |
Evaluation of histological post-mortem changes in farmed Atlantic salmon (Salmo salar L.) at different time intervals and storage temperatures |
title_short |
Evaluation of histological post-mortem changes in farmed Atlantic salmon (Salmo salar L.) at different time intervals and storage temperatures |
title_full |
Evaluation of histological post-mortem changes in farmed Atlantic salmon (Salmo salar L.) at different time intervals and storage temperatures |
title_fullStr |
Evaluation of histological post-mortem changes in farmed Atlantic salmon (Salmo salar L.) at different time intervals and storage temperatures |
title_full_unstemmed |
Evaluation of histological post-mortem changes in farmed Atlantic salmon (Salmo salar L.) at different time intervals and storage temperatures |
title_sort |
evaluation of histological post-mortem changes in farmed atlantic salmon (salmo salar l.) at different time intervals and storage temperatures |
publisher |
Wiley |
publishDate |
2022 |
url |
https://hdl.handle.net/10037/27210 https://doi.org/10.1111/jfd.13681 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Journal of Fish Diseases Furnesvik, Erkinharju, Hansen, Yousaf, Seternes. Evaluation of histological post-mortem changes in farmed Atlantic salmon (Salmo salar L.) at different time intervals and storage temperatures. Journal of Fish Diseases. 2022;45(10):1571-1580 FRIDAID 2054455 doi:10.1111/jfd.13681 0140-7775 1365-2761 https://hdl.handle.net/10037/27210 |
op_rights |
Attribution 4.0 International (CC BY 4.0) openAccess Copyright 2022 The Author(s) https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1111/jfd.13681 |
container_title |
Journal of Fish Diseases |
container_volume |
45 |
container_issue |
10 |
container_start_page |
1571 |
op_container_end_page |
1580 |
_version_ |
1766361890042150912 |