Impact of germ cell ablation on the activation of the brain–pituitary–gonadal axis in precocious Atlantic salmon (Salmo salar L.) males
The germ cells are essential for sexual reproduction by giving rise to the gametes, but the importance of germ cells for gonadal somatic functions varies among vertebrates. The RNA‐binding dead end (Dnd) protein is necessary for the specification and migration of primordial germ cells to the future...
Published in: | Molecular Reproduction and Development |
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Language: | English |
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Online Access: | https://hdl.handle.net/10037/27171 https://doi.org/10.1002/mrd.23635 |
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ftunivtroemsoe:oai:munin.uit.no:10037/27171 2023-05-15T15:30:36+02:00 Impact of germ cell ablation on the activation of the brain–pituitary–gonadal axis in precocious Atlantic salmon (Salmo salar L.) males Tveiten, Helge Karlsen, Kristian Thesslund, Tina Johansson, Gunhild Seljehaug Thiyagarajan, Dhivya Borra Andersen, Øivind 2022-07-13 https://hdl.handle.net/10037/27171 https://doi.org/10.1002/mrd.23635 eng eng Wiley Molecular Reproduction and Development Tveiten, Karlsen, Thesslund, Johansson, Thiyagarajan, Andersen. Impact of germ cell ablation on the activation of the brain–pituitary–gonadal axis in precocious Atlantic salmon (Salmo salar L.) males. Molecular Reproduction and Development. 2022 FRIDAID 2052098 doi:10.1002/mrd.23635 1040-452X 1098-2795 https://hdl.handle.net/10037/27171 Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) openAccess Copyright 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0 CC-BY-NC-ND Journal article Tidsskriftartikkel Peer reviewed publishedVersion 2022 ftunivtroemsoe https://doi.org/10.1002/mrd.23635 2022-11-03T00:01:18Z The germ cells are essential for sexual reproduction by giving rise to the gametes, but the importance of germ cells for gonadal somatic functions varies among vertebrates. The RNA‐binding dead end (Dnd) protein is necessary for the specification and migration of primordial germ cells to the future reproductive organs. Here, we ablated the gametes in Atlantic salmon males and females by microinjecting dnd antisense gapmer oligonucleotides at the zygotic stage. Precocious maturation was induced in above 50% of both germ cell‐depleted and intact fertile males, but not in females, by exposure to an off‐season photoperiod regime. Sterile and fertile males showed similar body growth, but maturing fish tended to be heavier than their immature counterparts. Pituitary fshβ messenger RNA levels strongly increased in maturing sterile and fertile males concomitant with the upregulated expression of Sertoli and Leydig cell markers. Plasma concentrations of 11‐ketotestosterone and testosterone in maturing sterile males were significantly higher than the basal levels in immature fish, but lower than those in maturing fertile males. The study demonstrates that germ cells are not a prerequisite for the activation of the brain–pituitary–gonad axis and sex steroidogenesis in Atlantic salmon males, but may be important for the maintenance of gonadal somatic functions. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Tromsø: Munin Open Research Archive Molecular Reproduction and Development 89 10 471 484 |
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Open Polar |
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University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
description |
The germ cells are essential for sexual reproduction by giving rise to the gametes, but the importance of germ cells for gonadal somatic functions varies among vertebrates. The RNA‐binding dead end (Dnd) protein is necessary for the specification and migration of primordial germ cells to the future reproductive organs. Here, we ablated the gametes in Atlantic salmon males and females by microinjecting dnd antisense gapmer oligonucleotides at the zygotic stage. Precocious maturation was induced in above 50% of both germ cell‐depleted and intact fertile males, but not in females, by exposure to an off‐season photoperiod regime. Sterile and fertile males showed similar body growth, but maturing fish tended to be heavier than their immature counterparts. Pituitary fshβ messenger RNA levels strongly increased in maturing sterile and fertile males concomitant with the upregulated expression of Sertoli and Leydig cell markers. Plasma concentrations of 11‐ketotestosterone and testosterone in maturing sterile males were significantly higher than the basal levels in immature fish, but lower than those in maturing fertile males. The study demonstrates that germ cells are not a prerequisite for the activation of the brain–pituitary–gonad axis and sex steroidogenesis in Atlantic salmon males, but may be important for the maintenance of gonadal somatic functions. |
format |
Article in Journal/Newspaper |
author |
Tveiten, Helge Karlsen, Kristian Thesslund, Tina Johansson, Gunhild Seljehaug Thiyagarajan, Dhivya Borra Andersen, Øivind |
spellingShingle |
Tveiten, Helge Karlsen, Kristian Thesslund, Tina Johansson, Gunhild Seljehaug Thiyagarajan, Dhivya Borra Andersen, Øivind Impact of germ cell ablation on the activation of the brain–pituitary–gonadal axis in precocious Atlantic salmon (Salmo salar L.) males |
author_facet |
Tveiten, Helge Karlsen, Kristian Thesslund, Tina Johansson, Gunhild Seljehaug Thiyagarajan, Dhivya Borra Andersen, Øivind |
author_sort |
Tveiten, Helge |
title |
Impact of germ cell ablation on the activation of the brain–pituitary–gonadal axis in precocious Atlantic salmon (Salmo salar L.) males |
title_short |
Impact of germ cell ablation on the activation of the brain–pituitary–gonadal axis in precocious Atlantic salmon (Salmo salar L.) males |
title_full |
Impact of germ cell ablation on the activation of the brain–pituitary–gonadal axis in precocious Atlantic salmon (Salmo salar L.) males |
title_fullStr |
Impact of germ cell ablation on the activation of the brain–pituitary–gonadal axis in precocious Atlantic salmon (Salmo salar L.) males |
title_full_unstemmed |
Impact of germ cell ablation on the activation of the brain–pituitary–gonadal axis in precocious Atlantic salmon (Salmo salar L.) males |
title_sort |
impact of germ cell ablation on the activation of the brain–pituitary–gonadal axis in precocious atlantic salmon (salmo salar l.) males |
publisher |
Wiley |
publishDate |
2022 |
url |
https://hdl.handle.net/10037/27171 https://doi.org/10.1002/mrd.23635 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Molecular Reproduction and Development Tveiten, Karlsen, Thesslund, Johansson, Thiyagarajan, Andersen. Impact of germ cell ablation on the activation of the brain–pituitary–gonadal axis in precocious Atlantic salmon (Salmo salar L.) males. Molecular Reproduction and Development. 2022 FRIDAID 2052098 doi:10.1002/mrd.23635 1040-452X 1098-2795 https://hdl.handle.net/10037/27171 |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) openAccess Copyright 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0 |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1002/mrd.23635 |
container_title |
Molecular Reproduction and Development |
container_volume |
89 |
container_issue |
10 |
container_start_page |
471 |
op_container_end_page |
484 |
_version_ |
1766361046858072064 |