Effect of viral RNA mimics and ISA virus infection on expression of key genes of the Atlantic salmon interferon system

• Main Author: Kileng, Øyvind
• Format: Doctoral or Postdoctoral Thesis
• Language: English
• Published: Universitetet i Tromsø 2008
• Subjects: VDP::Matematikk og naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474; VDP::Landbruks- og fiskerifag: 900::Fiskerifag: 920::Fiskehelse: 923; VDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474; VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923; Bergan; Rimstad; Kileng; Atlantic salmon
• Online Access: https://hdl.handle.net/10037/2467

The papers of the thesis are not available in Munin: 1. Kileng, Ø., Brundtland, M. I. and Robertsen, B.: «Infectious salmon anemia virus is a powerful inducer of key genes of the type I interferon system of Atlantic salmon, but is not inhibited by interferon», Fish & Shellfish immunology 23(2007); 378-389 (Elsevier - publisher's restriction). Available at http://dx.doi.org/10.1016/j.fsi.2006.11.011 2. Kileng, Ø., Albuquerque, A. and Robertsen, B.: «Induction of interferon system genes in Atlantic salmon by the imidazoquinoline S-27609, a ligand for Toll-like receptor 7», Fish & Shellfish immunology 24(2007); 514-522 (Elsevier - publisher's restriction). Available at http://dx.doi.org/10.1016/j.fsi.2007.10.005 3. García-Rosado, E., Markussen, T., Kileng, Ø., Baekkevold, E. S., Robertsen, B., Mjaaland, S. and Rimstad, E.: «Molecular and functional characterization of two infectious salmon anaemia virus (ISAV) proteins with type I interferon antagonizing activity», Virus Research 133(2008); 228-238 (Elsevier - publisher's restriction). Available at http://dx.doi.org/10.1016/j.virusres.2008.01.008 4. Kileng, Ø., Workenhe, S. T., Bergan, V. and Robertsen, B.: «An Atlantic salmon IRF-7 gene identified by analysis of sequence and function and its expression compared with a STAT1 gene». Manuscript, later published in Developmental and Comparative Immunology 33(2009); 18-27 (Elsevier), published version available at http://dx.doi.org/10.1016/j.dci.2008.07.020 We have studied the innate immune responses of Atlantic salmon and how this responds to stimulation and infection by the aquatic orthomyxovirus Infectious salmon anemia virus (ISAV). This work has focused on the Type I interferon (IFN) system which is critically important in the vertebrate immune response against viral infections. As a part of this work we have studied the Atlantic salmon IFN regulatory factor 7 (IRF7). In mammals IRF7 is the major regulator of IFN transcription in response to viral infections, and our results show that the salmon homolog ...