Secretome Profiling of Atlantic Salmon Head Kidney Leukocytes Highlights the Role of Phagocytes in the Immune Response to Soluble β-Glucan
b‐Glucans (BG) are glucose polymers which are produced in bacteria and fungi but not in vertebrate organisms. Being recognized by phagocytic leukocytes including macrophages and neutrophils through receptors such as dectin-1 and Complement receptor 3 (CR3), the BG are perceived by the innate immune...
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Online Access: | https://hdl.handle.net/10037/24014 https://doi.org/10.3389/fimmu.2021.736964 |
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ftunivtroemsoe:oai:munin.uit.no:10037/24014 2023-05-15T15:31:45+02:00 Secretome Profiling of Atlantic Salmon Head Kidney Leukocytes Highlights the Role of Phagocytes in the Immune Response to Soluble β-Glucan Iliev, Dimitar B. Strandskog, Guro Sobhkhez, Mehrdad Bruun, Jack A. Jørgensen, Jorunn B. 2021-11-30 https://hdl.handle.net/10037/24014 https://doi.org/10.3389/fimmu.2021.736964 eng eng Frontiers Media Frontiers in Immunology info:eu-repo/grantAgreement/RCN/FRIMEDBIO/230735/Norway/Exosomes from salmon (Salmo salar) leukocytes - composition, functions, biogenesis and immunodiagnostic potential// Iliev, Strandskog, Sobhkhez, Bruun, Jørgensen. Secretome Profiling of Atlantic Salmon Head Kidney Leukocytes Highlights the Role of Phagocytes in the Immune Response to Soluble β-Glucan. Frontiers in Immunology. 2021;12:1-14 FRIDAID 1972582 doi:10.3389/fimmu.2021.736964 1664-3224 https://hdl.handle.net/10037/24014 openAccess Copyright 2021 The Author(s) Journal article Tidsskriftartikkel Peer reviewed publishedVersion 2021 ftunivtroemsoe https://doi.org/10.3389/fimmu.2021.736964 2022-02-16T23:57:06Z b‐Glucans (BG) are glucose polymers which are produced in bacteria and fungi but not in vertebrate organisms. Being recognized by phagocytic leukocytes including macrophages and neutrophils through receptors such as dectin-1 and Complement receptor 3 (CR3), the BG are perceived by the innate immune system of vertebrates as foreign substances known as Pathogen Associated Molecular Patterns (PAMPs). The yeast-derived BG has been recognized for its potent biological activity and it is used as an immunomodulator in human and veterinary medicine. The goal of the current study was to characterize the immunostimulatory activity of soluble yeast BG in primary cultures of Atlantic salmon (Salmo salar) head kidney leukocytes (HKLs) in which phagocytic cell types including neutrophils and mononuclear phagocytes predominate. The effect of BG on the secretome of HKL cultures, including secretion of extracellular vesicles (EVs) and soluble protein55s was characterized through western blotting and mass spectrometry. The results demonstrate that, along with upregulation of proinflammatory genes, BG induces secretion of ubiquitinated proteins (UbP), MHCII-containing EVs from professional antigen presenting cells as well as proteins derived from granules of polymorphonuclear granulocytes (PMN). Among the most abundant proteins identified in BG-induced EVs were beta-2 integrin subunits, including CD18 and CD11 homologs, which highlights the role of salmon granulocytes and mononuclear phagocytes in the response to soluble BG. Overall, the current work advances the knowledge about the immunostimulatory activity of yeast BG on the salmon immune system by shedding light on the effect of this PAMP on the secretome of salmon leukocytes. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Tromsø: Munin Open Research Archive Frontiers in Immunology 12 |
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University of Tromsø: Munin Open Research Archive |
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ftunivtroemsoe |
language |
English |
description |
b‐Glucans (BG) are glucose polymers which are produced in bacteria and fungi but not in vertebrate organisms. Being recognized by phagocytic leukocytes including macrophages and neutrophils through receptors such as dectin-1 and Complement receptor 3 (CR3), the BG are perceived by the innate immune system of vertebrates as foreign substances known as Pathogen Associated Molecular Patterns (PAMPs). The yeast-derived BG has been recognized for its potent biological activity and it is used as an immunomodulator in human and veterinary medicine. The goal of the current study was to characterize the immunostimulatory activity of soluble yeast BG in primary cultures of Atlantic salmon (Salmo salar) head kidney leukocytes (HKLs) in which phagocytic cell types including neutrophils and mononuclear phagocytes predominate. The effect of BG on the secretome of HKL cultures, including secretion of extracellular vesicles (EVs) and soluble protein55s was characterized through western blotting and mass spectrometry. The results demonstrate that, along with upregulation of proinflammatory genes, BG induces secretion of ubiquitinated proteins (UbP), MHCII-containing EVs from professional antigen presenting cells as well as proteins derived from granules of polymorphonuclear granulocytes (PMN). Among the most abundant proteins identified in BG-induced EVs were beta-2 integrin subunits, including CD18 and CD11 homologs, which highlights the role of salmon granulocytes and mononuclear phagocytes in the response to soluble BG. Overall, the current work advances the knowledge about the immunostimulatory activity of yeast BG on the salmon immune system by shedding light on the effect of this PAMP on the secretome of salmon leukocytes. |
format |
Article in Journal/Newspaper |
author |
Iliev, Dimitar B. Strandskog, Guro Sobhkhez, Mehrdad Bruun, Jack A. Jørgensen, Jorunn B. |
spellingShingle |
Iliev, Dimitar B. Strandskog, Guro Sobhkhez, Mehrdad Bruun, Jack A. Jørgensen, Jorunn B. Secretome Profiling of Atlantic Salmon Head Kidney Leukocytes Highlights the Role of Phagocytes in the Immune Response to Soluble β-Glucan |
author_facet |
Iliev, Dimitar B. Strandskog, Guro Sobhkhez, Mehrdad Bruun, Jack A. Jørgensen, Jorunn B. |
author_sort |
Iliev, Dimitar B. |
title |
Secretome Profiling of Atlantic Salmon Head Kidney Leukocytes Highlights the Role of Phagocytes in the Immune Response to Soluble β-Glucan |
title_short |
Secretome Profiling of Atlantic Salmon Head Kidney Leukocytes Highlights the Role of Phagocytes in the Immune Response to Soluble β-Glucan |
title_full |
Secretome Profiling of Atlantic Salmon Head Kidney Leukocytes Highlights the Role of Phagocytes in the Immune Response to Soluble β-Glucan |
title_fullStr |
Secretome Profiling of Atlantic Salmon Head Kidney Leukocytes Highlights the Role of Phagocytes in the Immune Response to Soluble β-Glucan |
title_full_unstemmed |
Secretome Profiling of Atlantic Salmon Head Kidney Leukocytes Highlights the Role of Phagocytes in the Immune Response to Soluble β-Glucan |
title_sort |
secretome profiling of atlantic salmon head kidney leukocytes highlights the role of phagocytes in the immune response to soluble β-glucan |
publisher |
Frontiers Media |
publishDate |
2021 |
url |
https://hdl.handle.net/10037/24014 https://doi.org/10.3389/fimmu.2021.736964 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Frontiers in Immunology info:eu-repo/grantAgreement/RCN/FRIMEDBIO/230735/Norway/Exosomes from salmon (Salmo salar) leukocytes - composition, functions, biogenesis and immunodiagnostic potential// Iliev, Strandskog, Sobhkhez, Bruun, Jørgensen. Secretome Profiling of Atlantic Salmon Head Kidney Leukocytes Highlights the Role of Phagocytes in the Immune Response to Soluble β-Glucan. Frontiers in Immunology. 2021;12:1-14 FRIDAID 1972582 doi:10.3389/fimmu.2021.736964 1664-3224 https://hdl.handle.net/10037/24014 |
op_rights |
openAccess Copyright 2021 The Author(s) |
op_doi |
https://doi.org/10.3389/fimmu.2021.736964 |
container_title |
Frontiers in Immunology |
container_volume |
12 |
_version_ |
1766362275251224576 |