Issues of under-representation in quantitative DNA metabarcoding weaken the inference about diet of the tundra vole Microtus oeconomus
During the last decade, methods based on high-throughput sequencing such as DNA metabarcoding have opened up for a range of new questions in animal dietary studies. One of the major advantages of dietary metabarcoding resides in the potential to infer a quantitative relationship between sequence rea...
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ftunivtroemsoe:oai:munin.uit.no:10037/23178 2023-05-15T18:40:15+02:00 Issues of under-representation in quantitative DNA metabarcoding weaken the inference about diet of the tundra vole Microtus oeconomus Neby, Magne Kamenova, Stefaniya Devineau, Olivier Ims, Rolf Anker Soininen, Eeva M 2021-08-26 https://hdl.handle.net/10037/23178 https://doi.org/10.7717/peerj.11936 eng eng PeerJ PeerJ FRIDAID 1941054 doi:10.7717/peerj.11936 2167-8359 https://hdl.handle.net/10037/23178 openAccess Copyright 2021 The Author(s) VDP::Mathematics and natural science: 400::Zoology and botany: 480 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480 Journal article Tidsskriftartikkel Peer reviewed publishedVersion 2021 ftunivtroemsoe https://doi.org/10.7717/peerj.11936 2021-12-01T23:54:51Z During the last decade, methods based on high-throughput sequencing such as DNA metabarcoding have opened up for a range of new questions in animal dietary studies. One of the major advantages of dietary metabarcoding resides in the potential to infer a quantitative relationship between sequence read proportions and biomass of ingested food. However, this relationship’s robustness is highly dependent on the system under study, calling for case-specific assessments. Herbivorous small rodents often play important roles in the ecosystem, and the use of DNA metabarcoding for analyses of rodent diets is increasing. However, there has been no direct validation of the quantitative reliability of DNA metabarcoding for small rodents. Therefore, we used an experimental approach to assess the relationship between input plant biomass and sequence reads proportions from DNA metabarcoding in the tundra vole Microtus oeconomus. We found a weakly positive relationship between the number of high-throughput DNA sequences and the expected biomass proportions of food plants. The weak relationship was possibly caused by a systematic under-amplification of one of the three plant taxa fed. Generally, our results add to the growing evidence that case-specific validation studies are required to reliably make use of sequence read abundance as a proxy of relative food proportions in the diet. Article in Journal/Newspaper Tundra University of Tromsø: Munin Open Research Archive PeerJ 9 e11936 |
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University of Tromsø: Munin Open Research Archive |
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ftunivtroemsoe |
language |
English |
topic |
VDP::Mathematics and natural science: 400::Zoology and botany: 480 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480 |
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VDP::Mathematics and natural science: 400::Zoology and botany: 480 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480 Neby, Magne Kamenova, Stefaniya Devineau, Olivier Ims, Rolf Anker Soininen, Eeva M Issues of under-representation in quantitative DNA metabarcoding weaken the inference about diet of the tundra vole Microtus oeconomus |
topic_facet |
VDP::Mathematics and natural science: 400::Zoology and botany: 480 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480 |
description |
During the last decade, methods based on high-throughput sequencing such as DNA metabarcoding have opened up for a range of new questions in animal dietary studies. One of the major advantages of dietary metabarcoding resides in the potential to infer a quantitative relationship between sequence read proportions and biomass of ingested food. However, this relationship’s robustness is highly dependent on the system under study, calling for case-specific assessments. Herbivorous small rodents often play important roles in the ecosystem, and the use of DNA metabarcoding for analyses of rodent diets is increasing. However, there has been no direct validation of the quantitative reliability of DNA metabarcoding for small rodents. Therefore, we used an experimental approach to assess the relationship between input plant biomass and sequence reads proportions from DNA metabarcoding in the tundra vole Microtus oeconomus. We found a weakly positive relationship between the number of high-throughput DNA sequences and the expected biomass proportions of food plants. The weak relationship was possibly caused by a systematic under-amplification of one of the three plant taxa fed. Generally, our results add to the growing evidence that case-specific validation studies are required to reliably make use of sequence read abundance as a proxy of relative food proportions in the diet. |
format |
Article in Journal/Newspaper |
author |
Neby, Magne Kamenova, Stefaniya Devineau, Olivier Ims, Rolf Anker Soininen, Eeva M |
author_facet |
Neby, Magne Kamenova, Stefaniya Devineau, Olivier Ims, Rolf Anker Soininen, Eeva M |
author_sort |
Neby, Magne |
title |
Issues of under-representation in quantitative DNA metabarcoding weaken the inference about diet of the tundra vole Microtus oeconomus |
title_short |
Issues of under-representation in quantitative DNA metabarcoding weaken the inference about diet of the tundra vole Microtus oeconomus |
title_full |
Issues of under-representation in quantitative DNA metabarcoding weaken the inference about diet of the tundra vole Microtus oeconomus |
title_fullStr |
Issues of under-representation in quantitative DNA metabarcoding weaken the inference about diet of the tundra vole Microtus oeconomus |
title_full_unstemmed |
Issues of under-representation in quantitative DNA metabarcoding weaken the inference about diet of the tundra vole Microtus oeconomus |
title_sort |
issues of under-representation in quantitative dna metabarcoding weaken the inference about diet of the tundra vole microtus oeconomus |
publisher |
PeerJ |
publishDate |
2021 |
url |
https://hdl.handle.net/10037/23178 https://doi.org/10.7717/peerj.11936 |
genre |
Tundra |
genre_facet |
Tundra |
op_relation |
PeerJ FRIDAID 1941054 doi:10.7717/peerj.11936 2167-8359 https://hdl.handle.net/10037/23178 |
op_rights |
openAccess Copyright 2021 The Author(s) |
op_doi |
https://doi.org/10.7717/peerj.11936 |
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PeerJ |
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9 |
container_start_page |
e11936 |
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1766229534659575808 |