Microbial community structure associated to Arctic cold seeps
Cold seeps are locations on the seafloor where CH 4 migrates from reservoirs below sediments towards the atmosphere, sustaining thereby a high microbial and macrofaunal biomass and a diversity contrasting from the surrounding seafloor. The oxidation of methane and sulphide are typically the main sou...
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Format: | Doctoral or Postdoctoral Thesis |
Language: | English |
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UiT The Arctic University of Norway
2021
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Online Access: | https://hdl.handle.net/10037/22978 |
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ftunivtroemsoe:oai:munin.uit.no:10037/22978 |
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Open Polar |
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University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
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English |
topic |
VDP::Mathematics and natural science: 400::Basic biosciences: 470::General microbiology: 472 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell mikrobiologi: 472 VDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Marine biology: 497 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Ecology: 488 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Økologi: 488 DOKTOR-002 |
spellingShingle |
VDP::Mathematics and natural science: 400::Basic biosciences: 470::General microbiology: 472 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell mikrobiologi: 472 VDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Marine biology: 497 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Ecology: 488 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Økologi: 488 DOKTOR-002 Carrier, Vincent Microbial community structure associated to Arctic cold seeps |
topic_facet |
VDP::Mathematics and natural science: 400::Basic biosciences: 470::General microbiology: 472 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell mikrobiologi: 472 VDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Marine biology: 497 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Ecology: 488 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Økologi: 488 DOKTOR-002 |
description |
Cold seeps are locations on the seafloor where CH 4 migrates from reservoirs below sediments towards the atmosphere, sustaining thereby a high microbial and macrofaunal biomass and a diversity contrasting from the surrounding seafloor. The oxidation of methane and sulphide are typically the main sources of primary productivity of these ecosystems and have therefore gained a particular attention in the global oceans. Yet, despite the ubiquitous presence of these seeping sites and the presence of gas hydrates in the Arctic Ocean and its adjacent shelves, the impact of methane on benthic and pelagic microbial communities in this region have remained limited. Recently, five gas hydrate bearing mounds with ongoing methane seeping activity were discovered south of Svalbard, in the northern Barents Sea. In this PhD project, I studied changes in the structure of microbial communities, including both prokaryotes and eukaryotes, and geochemical profiles at these mounds to highlight key microbial groups and to provide insights on their ecological roles. Different niches were addressed, including: deep anaerobic sediments (Paper I and II); niches at the sediment surface at gas flare locations and within bacterial mats and siboglinid fields (Paper III); and above gas flares in the shallow shelf water column (Paper IV). The microbial biodiversity and the structure of communities were successfully identified for each of the habitats listed above. Our investigations revealed a microbial composition similar to other cold seeps: a predominance of archaeal anaerobic methanotrophs (ANME) and sulphate-reducing bacteria (SRB) in CH 4 -rich sediments, a higher abundance of methane oxidizing bacteria associated to the Methylococcales in the surface sediments and water column; and a co-occurrence of other commonly found prokaryotic groups. Yet, uncommon biological traits were also uncovered at these methane seeping sites: the anaerobic oxidation of methane was merely only driven by ANME-1 without the co-occurrence of a specific SRB clade; an abundant methanotroph with little genetic similarity in databases was detected; and a strong niche differentiation of sulphide-oxidizing bacteria within the different bacterial mats. This project has thereby extended our knowledge on the microbial biodiversity at Arctic cold seeps and opened further future research perspective toward microbial activity and metabolism at these high latitudes. |
format |
Doctoral or Postdoctoral Thesis |
author |
Carrier, Vincent |
author_facet |
Carrier, Vincent |
author_sort |
Carrier, Vincent |
title |
Microbial community structure associated to Arctic cold seeps |
title_short |
Microbial community structure associated to Arctic cold seeps |
title_full |
Microbial community structure associated to Arctic cold seeps |
title_fullStr |
Microbial community structure associated to Arctic cold seeps |
title_full_unstemmed |
Microbial community structure associated to Arctic cold seeps |
title_sort |
microbial community structure associated to arctic cold seeps |
publisher |
UiT The Arctic University of Norway |
publishDate |
2021 |
url |
https://hdl.handle.net/10037/22978 |
geographic |
Arctic Arctic Ocean Barents Sea Svalbard |
geographic_facet |
Arctic Arctic Ocean Barents Sea Svalbard |
genre |
Arctic Arctic Arctic Ocean Barents Sea Svalbard |
genre_facet |
Arctic Arctic Arctic Ocean Barents Sea Svalbard |
op_relation |
Paper I: Carrier, V., Svenning, M.M., Gründger, F., Niemann, H., Dessandier, P.A., Panieri, G. & Kalenitchenko, D. (2020). The Impact of Methane on Microbial Communities at Marine Arctic Gas Hydrate Bearing Sediment. Frontiers in Microbiology, 11 , 1932. Also available in Munin at https://hdl.handle.net/10037/19507 . Paper II: Gründger, F., Carrier, V., Svenning, M.M., Panieri, G., Vonnahme, T.R., Klasek, S. & Niemann, H. (2019). Methane-fuelled biofilms predominantly composed of methanotrophic ANME-1 in Arctic gas hydrate-related sediments. Scientific Reports, 9 , 9725. Also available in Munin at https://hdl.handle.net/10037/16225 . Paper III: Carrier V., Svenning, M.M., Niemann, H., Gründger, F.F. & Kalenitchenko, D. Niche differentiation of prokaryotic communities and aerobic methanotrophs in surface sediments of an Arctic cold seep. (Manuscript). Paper IV: Gründger, F., Probandt, D., Knittel, K., Carrier, V., Kalenitchenko, D., Silyakova, A., … Niemann, H. (2021). Seasonal shifts of microbial methane oxidation in Arctic shelf waters above gas seeps. Limnology and Oceanography, 66 (5), 1896-1914. Also available in Munin at https://hdl.handle.net/10037/21556 . info:eu-repo/grantAgreement/RCN/SFF/223259/Norway/Centre for Arctic Gas Hydrate, Environment and Climate/CAGE/ 978-82-8266-206-2 https://hdl.handle.net/10037/22978 |
op_rights |
openAccess Copyright 2021 The Author(s) |
_version_ |
1766296110403420160 |
spelling |
ftunivtroemsoe:oai:munin.uit.no:10037/22978 2023-05-15T14:23:36+02:00 Microbial community structure associated to Arctic cold seeps Carrier, Vincent 2021-11-29 https://hdl.handle.net/10037/22978 eng eng UiT The Arctic University of Norway UiT Norges arktiske universitet Paper I: Carrier, V., Svenning, M.M., Gründger, F., Niemann, H., Dessandier, P.A., Panieri, G. & Kalenitchenko, D. (2020). The Impact of Methane on Microbial Communities at Marine Arctic Gas Hydrate Bearing Sediment. Frontiers in Microbiology, 11 , 1932. Also available in Munin at https://hdl.handle.net/10037/19507 . Paper II: Gründger, F., Carrier, V., Svenning, M.M., Panieri, G., Vonnahme, T.R., Klasek, S. & Niemann, H. (2019). Methane-fuelled biofilms predominantly composed of methanotrophic ANME-1 in Arctic gas hydrate-related sediments. Scientific Reports, 9 , 9725. Also available in Munin at https://hdl.handle.net/10037/16225 . Paper III: Carrier V., Svenning, M.M., Niemann, H., Gründger, F.F. & Kalenitchenko, D. Niche differentiation of prokaryotic communities and aerobic methanotrophs in surface sediments of an Arctic cold seep. (Manuscript). Paper IV: Gründger, F., Probandt, D., Knittel, K., Carrier, V., Kalenitchenko, D., Silyakova, A., … Niemann, H. (2021). Seasonal shifts of microbial methane oxidation in Arctic shelf waters above gas seeps. Limnology and Oceanography, 66 (5), 1896-1914. Also available in Munin at https://hdl.handle.net/10037/21556 . info:eu-repo/grantAgreement/RCN/SFF/223259/Norway/Centre for Arctic Gas Hydrate, Environment and Climate/CAGE/ 978-82-8266-206-2 https://hdl.handle.net/10037/22978 openAccess Copyright 2021 The Author(s) VDP::Mathematics and natural science: 400::Basic biosciences: 470::General microbiology: 472 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell mikrobiologi: 472 VDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Marine biology: 497 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Ecology: 488 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Økologi: 488 DOKTOR-002 Doctoral thesis Doktorgradsavhandling 2021 ftunivtroemsoe 2021-11-17T23:54:38Z Cold seeps are locations on the seafloor where CH 4 migrates from reservoirs below sediments towards the atmosphere, sustaining thereby a high microbial and macrofaunal biomass and a diversity contrasting from the surrounding seafloor. The oxidation of methane and sulphide are typically the main sources of primary productivity of these ecosystems and have therefore gained a particular attention in the global oceans. Yet, despite the ubiquitous presence of these seeping sites and the presence of gas hydrates in the Arctic Ocean and its adjacent shelves, the impact of methane on benthic and pelagic microbial communities in this region have remained limited. Recently, five gas hydrate bearing mounds with ongoing methane seeping activity were discovered south of Svalbard, in the northern Barents Sea. In this PhD project, I studied changes in the structure of microbial communities, including both prokaryotes and eukaryotes, and geochemical profiles at these mounds to highlight key microbial groups and to provide insights on their ecological roles. Different niches were addressed, including: deep anaerobic sediments (Paper I and II); niches at the sediment surface at gas flare locations and within bacterial mats and siboglinid fields (Paper III); and above gas flares in the shallow shelf water column (Paper IV). The microbial biodiversity and the structure of communities were successfully identified for each of the habitats listed above. Our investigations revealed a microbial composition similar to other cold seeps: a predominance of archaeal anaerobic methanotrophs (ANME) and sulphate-reducing bacteria (SRB) in CH 4 -rich sediments, a higher abundance of methane oxidizing bacteria associated to the Methylococcales in the surface sediments and water column; and a co-occurrence of other commonly found prokaryotic groups. Yet, uncommon biological traits were also uncovered at these methane seeping sites: the anaerobic oxidation of methane was merely only driven by ANME-1 without the co-occurrence of a specific SRB clade; an abundant methanotroph with little genetic similarity in databases was detected; and a strong niche differentiation of sulphide-oxidizing bacteria within the different bacterial mats. This project has thereby extended our knowledge on the microbial biodiversity at Arctic cold seeps and opened further future research perspective toward microbial activity and metabolism at these high latitudes. Doctoral or Postdoctoral Thesis Arctic Arctic Arctic Ocean Barents Sea Svalbard University of Tromsø: Munin Open Research Archive Arctic Arctic Ocean Barents Sea Svalbard |