Developmental effects of embryonic exposure to a water-soluble fraction of crude oil on early life stages of capelin (Mallotus villosus)
The rise in offshore oil and gas operations, maritime shipping and tourism in northern latitudes enhance the risk of petroleum pollution and anthropogenic impacts of oil-related compounds on sub-Arctic and Arctic organisms. In particular, there is a need to investigate the potential adverse effects...
Main Author: | |
---|---|
Format: | Master Thesis |
Language: | English |
Published: |
UiT Norges arktiske universitet
2020
|
Subjects: | |
Online Access: | https://hdl.handle.net/10037/22961 |
id |
ftunivtroemsoe:oai:munin.uit.no:10037/22961 |
---|---|
record_format |
openpolar |
spelling |
ftunivtroemsoe:oai:munin.uit.no:10037/22961 2023-05-15T15:03:41+02:00 Developmental effects of embryonic exposure to a water-soluble fraction of crude oil on early life stages of capelin (Mallotus villosus) Granlund, Adina Cassandra 2020-05-15 https://hdl.handle.net/10037/22961 eng eng UiT Norges arktiske universitet UiT The Arctic University of Norway https://hdl.handle.net/10037/22961 openAccess Copyright 2020 The Author(s) VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Marine biology: 497 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Økotoksikologi: 489 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Ecotoxicology: 489 BIO-3950 Master thesis Mastergradsoppgave 2020 ftunivtroemsoe 2021-11-17T23:54:38Z The rise in offshore oil and gas operations, maritime shipping and tourism in northern latitudes enhance the risk of petroleum pollution and anthropogenic impacts of oil-related compounds on sub-Arctic and Arctic organisms. In particular, there is a need to investigate the potential adverse effects of petroleum to the early life stages of capelin ( Mallotus villosus ), an important forage and commercial fish species that spawn along the coast of Northern Norway. In this study, newly fertilized capelin embryos were exposed to five concentrations (control, low, medium, high and extra high) of a water-soluble fraction (WSF) of crude oil using oiled gravel columns loaded with either clean gravel (control) or gravel loaded with between 0.19 and 6 g oil/kg gravel (low to extra high groups). Embryos were exposed to decreasing crude oil WSF until hatch (25 days post fertilization) and larvae were followed in clean water until 58 days post fertilization. The initial aqueous total polycyclic aromatic hydrocarbon (PAHs) levels (sum of 44 PAHs) were ranging from 0.072 to 19.25 μg/L in the five treatment groups and decreased exponentially over time. None of the measured endpoints regarding embryo development and mortality, larval length, growth rate, cardiac activity, arrhythmia, and larval mortality showed any dose-dependent effects. Our results suggest that the early life stages of capelin are more robust to crude oil exposure than similar life stages of other fish species. The capelins demersal eggs properties, primarily the double-layered chorion was hypothesized to be a possible explanation for this trend. To verify this hypothesis, further investigation of accumulated levels in embryos and how eggs morphology affects accumulation could be investigated. Master Thesis Arctic Northern Norway University of Tromsø: Munin Open Research Archive Arctic Norway |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Marine biology: 497 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Økotoksikologi: 489 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Ecotoxicology: 489 BIO-3950 |
spellingShingle |
VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Marine biology: 497 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Økotoksikologi: 489 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Ecotoxicology: 489 BIO-3950 Granlund, Adina Cassandra Developmental effects of embryonic exposure to a water-soluble fraction of crude oil on early life stages of capelin (Mallotus villosus) |
topic_facet |
VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Marine biology: 497 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Økotoksikologi: 489 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Ecotoxicology: 489 BIO-3950 |
description |
The rise in offshore oil and gas operations, maritime shipping and tourism in northern latitudes enhance the risk of petroleum pollution and anthropogenic impacts of oil-related compounds on sub-Arctic and Arctic organisms. In particular, there is a need to investigate the potential adverse effects of petroleum to the early life stages of capelin ( Mallotus villosus ), an important forage and commercial fish species that spawn along the coast of Northern Norway. In this study, newly fertilized capelin embryos were exposed to five concentrations (control, low, medium, high and extra high) of a water-soluble fraction (WSF) of crude oil using oiled gravel columns loaded with either clean gravel (control) or gravel loaded with between 0.19 and 6 g oil/kg gravel (low to extra high groups). Embryos were exposed to decreasing crude oil WSF until hatch (25 days post fertilization) and larvae were followed in clean water until 58 days post fertilization. The initial aqueous total polycyclic aromatic hydrocarbon (PAHs) levels (sum of 44 PAHs) were ranging from 0.072 to 19.25 μg/L in the five treatment groups and decreased exponentially over time. None of the measured endpoints regarding embryo development and mortality, larval length, growth rate, cardiac activity, arrhythmia, and larval mortality showed any dose-dependent effects. Our results suggest that the early life stages of capelin are more robust to crude oil exposure than similar life stages of other fish species. The capelins demersal eggs properties, primarily the double-layered chorion was hypothesized to be a possible explanation for this trend. To verify this hypothesis, further investigation of accumulated levels in embryos and how eggs morphology affects accumulation could be investigated. |
format |
Master Thesis |
author |
Granlund, Adina Cassandra |
author_facet |
Granlund, Adina Cassandra |
author_sort |
Granlund, Adina Cassandra |
title |
Developmental effects of embryonic exposure to a water-soluble fraction of crude oil on early life stages of capelin (Mallotus villosus) |
title_short |
Developmental effects of embryonic exposure to a water-soluble fraction of crude oil on early life stages of capelin (Mallotus villosus) |
title_full |
Developmental effects of embryonic exposure to a water-soluble fraction of crude oil on early life stages of capelin (Mallotus villosus) |
title_fullStr |
Developmental effects of embryonic exposure to a water-soluble fraction of crude oil on early life stages of capelin (Mallotus villosus) |
title_full_unstemmed |
Developmental effects of embryonic exposure to a water-soluble fraction of crude oil on early life stages of capelin (Mallotus villosus) |
title_sort |
developmental effects of embryonic exposure to a water-soluble fraction of crude oil on early life stages of capelin (mallotus villosus) |
publisher |
UiT Norges arktiske universitet |
publishDate |
2020 |
url |
https://hdl.handle.net/10037/22961 |
geographic |
Arctic Norway |
geographic_facet |
Arctic Norway |
genre |
Arctic Northern Norway |
genre_facet |
Arctic Northern Norway |
op_relation |
https://hdl.handle.net/10037/22961 |
op_rights |
openAccess Copyright 2020 The Author(s) |
_version_ |
1766335542096560128 |