Transcriptome responses in polar cod (Boreogadus saida) liver slice culture exposed to benzo[a]pyrene and ethynylestradiol: insights into anti-estrogenic effects

Polar cod ( Boreogadus saida ) is a key species in the arctic marine ecosystem vulnerable to effects of pollution, particularly from petroleum related activities. To facilitate studying the effects of those pollutants, we adapted a precision-cut liver slice culture protocol for this species. Using t...

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Published in:Toxicology in Vitro
Main Authors: Yadetie, Fekadu, Brun, Nadja R, Vieweg, Ireen, Nahrgang, Jasmine, Karlsen, Odd André, Goksøyr, Anders
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier 2021
Subjects:
Online Access:https://hdl.handle.net/10037/22132
https://doi.org/10.1016/j.tiv.2021.105193
id ftunivtroemsoe:oai:munin.uit.no:10037/22132
record_format openpolar
spelling ftunivtroemsoe:oai:munin.uit.no:10037/22132 2023-05-15T15:09:38+02:00 Transcriptome responses in polar cod (Boreogadus saida) liver slice culture exposed to benzo[a]pyrene and ethynylestradiol: insights into anti-estrogenic effects Yadetie, Fekadu Brun, Nadja R Vieweg, Ireen Nahrgang, Jasmine Karlsen, Odd André Goksøyr, Anders 2021-05-17 https://hdl.handle.net/10037/22132 https://doi.org/10.1016/j.tiv.2021.105193 eng eng Elsevier Toxicology in Vitro Norges forskningsråd: 244564 Norges forskningsråd: 248840 Norges forskningsråd: 276730 info:eu-repo/grantAgreement/RCN/NANSEN/276730/Norway/The Nansen Legacy// info:eu-repo/grantAgreement/RCN/BIOTEK2021/248840/Norway/DL: dCod 1.0 -decoding systems toxicology of cod (Gadus morhua) -environmental genomics for ecosystem quality monitoring and risk assessment/dCod 1.0: systemtoksikologi hos torsk/dCod 1.0/ info:eu-repo/grantAgreement/RCN/MARINFORSK/244564/Norway/iCod 2.0: Integrative environmental genomics of Atlantic cod (Gadus morhua); a holistic approach to characterize the biological effects of/iCod 2.0: en miljøgenomisk tilnærming med Atlanterhavstorsk for å karakterisere effekter av "nye" miljøgifter og blandingseffekter/iCod 2.0/ https://www.sciencedirect.com/science/article/pii/S0887233321001181 Yadetie F, Brun NR, Vieweg I, Nahrgang J, Karlsen OA, Goksøyr A. Transcriptome responses in polar cod (Boreogadus saida) liver slice culture exposed to benzo[a]pyrene and ethynylestradiol: insights into anti-estrogenic effects. Toxicology in Vitro. 2021;75(105193) FRIDAID 1912897 https://doi.org/10.1016/j.tiv.2021.105193 0887-2333 1879-3177 https://hdl.handle.net/10037/22132 openAccess Copyright 2021 The Author(s) VDP::Mathematics and natural science: 400 VDP::Matematikk og Naturvitenskap: 400 Journal article Tidsskriftartikkel Peer reviewed publishedVersion 2021 ftunivtroemsoe https://doi.org/10.1016/j.tiv.2021.105193 2021-08-25T22:53:41Z Polar cod ( Boreogadus saida ) is a key species in the arctic marine ecosystem vulnerable to effects of pollution, particularly from petroleum related activities. To facilitate studying the effects of those pollutants, we adapted a precision-cut liver slice culture protocol for this species. Using this system on board a research vessel, we studied gene expression in liver slice after exposure to the polycyclic aromatic hydrocarbon (PAH) benzo[a]pyrene (BaP), ethynylestradiol (EE2), and their mixtures, to map their molecular targets and examine possible anti-estrogenic effects of BaP. The exposure experiments were performed with BaP alone (0.1, 1, and 10 μM) or in combination with low concentrations of EE2 (5 nM) to mimic physiological estradiol levels in early vitellogenic female fish. Transcriptome analysis (RNA-seq) was performed after 72 h exposure in culture to map the genes and cellular pathways affected. The results provide a view of global transcriptome responses to BaP and EE2, which resulted in enrichment of many pathways such as the aryl hydrocarbon (Ahr) and estrogen receptor pathways. In the mixture exposure, BaP resulted in anti-estrogenic effects, shown by attenuation of EE2 activated transcription of many estrogen target genes. The results from this ex vivo experiment suggest that pollutants that activate the Ahr pathway such as the PAH compound BaP can result in anti-estrogenic effects that may lead to endocrine disruption in polar cod. Article in Journal/Newspaper Arctic Boreogadus saida polar cod University of Tromsø: Munin Open Research Archive Arctic Toxicology in Vitro 75 105193
institution Open Polar
collection University of Tromsø: Munin Open Research Archive
op_collection_id ftunivtroemsoe
language English
topic VDP::Mathematics and natural science: 400
VDP::Matematikk og Naturvitenskap: 400
spellingShingle VDP::Mathematics and natural science: 400
VDP::Matematikk og Naturvitenskap: 400
Yadetie, Fekadu
Brun, Nadja R
Vieweg, Ireen
Nahrgang, Jasmine
Karlsen, Odd André
Goksøyr, Anders
Transcriptome responses in polar cod (Boreogadus saida) liver slice culture exposed to benzo[a]pyrene and ethynylestradiol: insights into anti-estrogenic effects
topic_facet VDP::Mathematics and natural science: 400
VDP::Matematikk og Naturvitenskap: 400
description Polar cod ( Boreogadus saida ) is a key species in the arctic marine ecosystem vulnerable to effects of pollution, particularly from petroleum related activities. To facilitate studying the effects of those pollutants, we adapted a precision-cut liver slice culture protocol for this species. Using this system on board a research vessel, we studied gene expression in liver slice after exposure to the polycyclic aromatic hydrocarbon (PAH) benzo[a]pyrene (BaP), ethynylestradiol (EE2), and their mixtures, to map their molecular targets and examine possible anti-estrogenic effects of BaP. The exposure experiments were performed with BaP alone (0.1, 1, and 10 μM) or in combination with low concentrations of EE2 (5 nM) to mimic physiological estradiol levels in early vitellogenic female fish. Transcriptome analysis (RNA-seq) was performed after 72 h exposure in culture to map the genes and cellular pathways affected. The results provide a view of global transcriptome responses to BaP and EE2, which resulted in enrichment of many pathways such as the aryl hydrocarbon (Ahr) and estrogen receptor pathways. In the mixture exposure, BaP resulted in anti-estrogenic effects, shown by attenuation of EE2 activated transcription of many estrogen target genes. The results from this ex vivo experiment suggest that pollutants that activate the Ahr pathway such as the PAH compound BaP can result in anti-estrogenic effects that may lead to endocrine disruption in polar cod.
format Article in Journal/Newspaper
author Yadetie, Fekadu
Brun, Nadja R
Vieweg, Ireen
Nahrgang, Jasmine
Karlsen, Odd André
Goksøyr, Anders
author_facet Yadetie, Fekadu
Brun, Nadja R
Vieweg, Ireen
Nahrgang, Jasmine
Karlsen, Odd André
Goksøyr, Anders
author_sort Yadetie, Fekadu
title Transcriptome responses in polar cod (Boreogadus saida) liver slice culture exposed to benzo[a]pyrene and ethynylestradiol: insights into anti-estrogenic effects
title_short Transcriptome responses in polar cod (Boreogadus saida) liver slice culture exposed to benzo[a]pyrene and ethynylestradiol: insights into anti-estrogenic effects
title_full Transcriptome responses in polar cod (Boreogadus saida) liver slice culture exposed to benzo[a]pyrene and ethynylestradiol: insights into anti-estrogenic effects
title_fullStr Transcriptome responses in polar cod (Boreogadus saida) liver slice culture exposed to benzo[a]pyrene and ethynylestradiol: insights into anti-estrogenic effects
title_full_unstemmed Transcriptome responses in polar cod (Boreogadus saida) liver slice culture exposed to benzo[a]pyrene and ethynylestradiol: insights into anti-estrogenic effects
title_sort transcriptome responses in polar cod (boreogadus saida) liver slice culture exposed to benzo[a]pyrene and ethynylestradiol: insights into anti-estrogenic effects
publisher Elsevier
publishDate 2021
url https://hdl.handle.net/10037/22132
https://doi.org/10.1016/j.tiv.2021.105193
geographic Arctic
geographic_facet Arctic
genre Arctic
Boreogadus saida
polar cod
genre_facet Arctic
Boreogadus saida
polar cod
op_relation Toxicology in Vitro
Norges forskningsråd: 244564
Norges forskningsråd: 248840
Norges forskningsråd: 276730
info:eu-repo/grantAgreement/RCN/NANSEN/276730/Norway/The Nansen Legacy//
info:eu-repo/grantAgreement/RCN/BIOTEK2021/248840/Norway/DL: dCod 1.0 -decoding systems toxicology of cod (Gadus morhua) -environmental genomics for ecosystem quality monitoring and risk assessment/dCod 1.0: systemtoksikologi hos torsk/dCod 1.0/
info:eu-repo/grantAgreement/RCN/MARINFORSK/244564/Norway/iCod 2.0: Integrative environmental genomics of Atlantic cod (Gadus morhua); a holistic approach to characterize the biological effects of/iCod 2.0: en miljøgenomisk tilnærming med Atlanterhavstorsk for å karakterisere effekter av "nye" miljøgifter og blandingseffekter/iCod 2.0/
https://www.sciencedirect.com/science/article/pii/S0887233321001181
Yadetie F, Brun NR, Vieweg I, Nahrgang J, Karlsen OA, Goksøyr A. Transcriptome responses in polar cod (Boreogadus saida) liver slice culture exposed to benzo[a]pyrene and ethynylestradiol: insights into anti-estrogenic effects. Toxicology in Vitro. 2021;75(105193)
FRIDAID 1912897
https://doi.org/10.1016/j.tiv.2021.105193
0887-2333
1879-3177
https://hdl.handle.net/10037/22132
op_rights openAccess
Copyright 2021 The Author(s)
op_doi https://doi.org/10.1016/j.tiv.2021.105193
container_title Toxicology in Vitro
container_volume 75
container_start_page 105193
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