Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet
Conservation strategies centered around species habitat protection rely on species’ dietary information. One species at the focal point of conservation efforts is the herbivorous grouse, the western capercaillie (Tetrao urogallus), which is an indicator species for forest biodiversity conservation....
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2021
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Online Access: | https://hdl.handle.net/10037/22006 https://doi.org/10.1002/edn3.237 |
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ftunivtroemsoe:oai:munin.uit.no:10037/22006 2023-05-15T14:26:19+02:00 Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet Chua, Physilia Y. S. Lammers, Youri Menoni, Emmanuel Ekrem, Torbjørn Bohmann, Kristine Boessenkool, Sanne Alsos, Inger Greve 2021-07-13 https://hdl.handle.net/10037/22006 https://doi.org/10.1002/edn3.237 eng eng Wiley Environmental DNA info:eu-repo/grantAgreement/EC/Plant.ID/314449/Norway/Arctic ecosystem impact assessment of oil in ice under climate change// Chua PYS, Lammers Y, Menoni, Ekrem T, Bohmann K, Boessenkool S, Alsos IGA. Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet. Environmental DNA. 2021 FRIDAID 1922232 doi:10.1002/edn3.237 2637-4943 https://hdl.handle.net/10037/22006 openAccess Copyright 2021 The Author(s) VDP::Mathematics and natural science: 400 VDP::Matematikk og Naturvitenskap: 400 Journal article Tidsskriftartikkel Peer reviewed publishedVersion 2021 ftunivtroemsoe https://doi.org/10.1002/edn3.237 2021-08-18T22:53:41Z Conservation strategies centered around species habitat protection rely on species’ dietary information. One species at the focal point of conservation efforts is the herbivorous grouse, the western capercaillie (Tetrao urogallus), which is an indicator species for forest biodiversity conservation. Non-molecular means used to study their diet are time-consuming and at low taxonomic resolution. This delays the implementation of conservation strategies including resource protection due to uncertainty about its diet. Thus, limited knowledge on diet is hampering conservation efforts. Here, we use non-invasive environmental DNA (eDNA) metabarcoding on DNA extracted from faces to present the first large-scale molecular dietary analysis of capercaillies. Facal samples were collected from seven populations located in Norway (Finnmark, Troms, Trøndelag, Innlandet) and France (Vosges, Jura, Pyrenees) (n = 172). We detected 122 plant taxa belonging to 46 plant families of which 37.7% of the detected taxa could be identified at species level. The average dietary richness of each sample was 7 ± 5 SD taxa. The most frequently occurring plant groups with the highest relative read abundance (RRA) were trees and dwarf shrubs, in particular, Pinus and Vaccinium myrtillus, respectively. There was a difference in dietary composition (RRA) between samples collected from the different locations (adonis pseudo F5,86 = 11.01, r2 = 0.17, p = 0.001) and seasons (adonis pseudo F2,03 = 0.64, r2 = 0.01, p = 0.036). Dietary composition also differed between sexes at each location (adonis pseudo F1,47 = 2.77, r2 = 0.04, p = 0.024), although not significant for all data combined. In total, 35 taxa (36.8% of taxa recorded) were new capercaillie food items compared with existing knowledge from non-molecular means. The non-invasive molecular dietary analysis applied in this study provides new ecological information of capercaillies’ diet, improving our understanding of adequate habitat required for their conservation. Article in Journal/Newspaper Arctic Finnmark Finnmark Troms University of Tromsø: Munin Open Research Archive Jura ENVELOPE(13.501,13.501,68.062,68.062) Norway Environmental DNA |
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Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Mathematics and natural science: 400 VDP::Matematikk og Naturvitenskap: 400 |
spellingShingle |
VDP::Mathematics and natural science: 400 VDP::Matematikk og Naturvitenskap: 400 Chua, Physilia Y. S. Lammers, Youri Menoni, Emmanuel Ekrem, Torbjørn Bohmann, Kristine Boessenkool, Sanne Alsos, Inger Greve Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet |
topic_facet |
VDP::Mathematics and natural science: 400 VDP::Matematikk og Naturvitenskap: 400 |
description |
Conservation strategies centered around species habitat protection rely on species’ dietary information. One species at the focal point of conservation efforts is the herbivorous grouse, the western capercaillie (Tetrao urogallus), which is an indicator species for forest biodiversity conservation. Non-molecular means used to study their diet are time-consuming and at low taxonomic resolution. This delays the implementation of conservation strategies including resource protection due to uncertainty about its diet. Thus, limited knowledge on diet is hampering conservation efforts. Here, we use non-invasive environmental DNA (eDNA) metabarcoding on DNA extracted from faces to present the first large-scale molecular dietary analysis of capercaillies. Facal samples were collected from seven populations located in Norway (Finnmark, Troms, Trøndelag, Innlandet) and France (Vosges, Jura, Pyrenees) (n = 172). We detected 122 plant taxa belonging to 46 plant families of which 37.7% of the detected taxa could be identified at species level. The average dietary richness of each sample was 7 ± 5 SD taxa. The most frequently occurring plant groups with the highest relative read abundance (RRA) were trees and dwarf shrubs, in particular, Pinus and Vaccinium myrtillus, respectively. There was a difference in dietary composition (RRA) between samples collected from the different locations (adonis pseudo F5,86 = 11.01, r2 = 0.17, p = 0.001) and seasons (adonis pseudo F2,03 = 0.64, r2 = 0.01, p = 0.036). Dietary composition also differed between sexes at each location (adonis pseudo F1,47 = 2.77, r2 = 0.04, p = 0.024), although not significant for all data combined. In total, 35 taxa (36.8% of taxa recorded) were new capercaillie food items compared with existing knowledge from non-molecular means. The non-invasive molecular dietary analysis applied in this study provides new ecological information of capercaillies’ diet, improving our understanding of adequate habitat required for their conservation. |
format |
Article in Journal/Newspaper |
author |
Chua, Physilia Y. S. Lammers, Youri Menoni, Emmanuel Ekrem, Torbjørn Bohmann, Kristine Boessenkool, Sanne Alsos, Inger Greve |
author_facet |
Chua, Physilia Y. S. Lammers, Youri Menoni, Emmanuel Ekrem, Torbjørn Bohmann, Kristine Boessenkool, Sanne Alsos, Inger Greve |
author_sort |
Chua, Physilia Y. S. |
title |
Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet |
title_short |
Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet |
title_full |
Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet |
title_fullStr |
Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet |
title_full_unstemmed |
Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet |
title_sort |
molecular dietary analyses of western capercaillies (tetrao urogallus) reveal a diverse diet |
publisher |
Wiley |
publishDate |
2021 |
url |
https://hdl.handle.net/10037/22006 https://doi.org/10.1002/edn3.237 |
long_lat |
ENVELOPE(13.501,13.501,68.062,68.062) |
geographic |
Jura Norway |
geographic_facet |
Jura Norway |
genre |
Arctic Finnmark Finnmark Troms |
genre_facet |
Arctic Finnmark Finnmark Troms |
op_relation |
Environmental DNA info:eu-repo/grantAgreement/EC/Plant.ID/314449/Norway/Arctic ecosystem impact assessment of oil in ice under climate change// Chua PYS, Lammers Y, Menoni, Ekrem T, Bohmann K, Boessenkool S, Alsos IGA. Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet. Environmental DNA. 2021 FRIDAID 1922232 doi:10.1002/edn3.237 2637-4943 https://hdl.handle.net/10037/22006 |
op_rights |
openAccess Copyright 2021 The Author(s) |
op_doi |
https://doi.org/10.1002/edn3.237 |
container_title |
Environmental DNA |
_version_ |
1766298823226818560 |