Analysing diet of small herbivores : the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures
Background: In order to understand the role of herbivores in trophic webs, it is essential to know what they feed on. Diet analysis is, however, a challenge in many small herbivores with a secretive life style. In this paper, we compare novel (high-throughput pyrosequencing) DNA barcoding technology...
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ftunivtroemsoe:oai:munin.uit.no:10037/2195 2024-06-02T08:15:02+00:00 Analysing diet of small herbivores : the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures Valentini, Alice Soininen, Eeva Marjatta Coissac, Eric Ims, Rolf Anker Miquel, Christian Yoccoz, Nigel Gilles Gielly, Ludovic Brochmann, Christian Brysting, Anne K. Sønstebø, Jørn H. Taberlet, Pierre 2009-08-20 250844 bytes application/pdf https://hdl.handle.net/10037/2195 eng eng BioMed Central Frontiers in Zoology 2009, 6:16 https://hdl.handle.net/10037/2195 URN:NBN:no-uit_munin_1947 openAccess VDP::Mathematics and natural science: 400 Journal article Tidsskriftartikkel Peer reviewed 2009 ftunivtroemsoe 2024-05-07T08:41:49Z Background: In order to understand the role of herbivores in trophic webs, it is essential to know what they feed on. Diet analysis is, however, a challenge in many small herbivores with a secretive life style. In this paper, we compare novel (high-throughput pyrosequencing) DNA barcoding technology for plant mixture with traditional microhistological method. We analysed stomach contents of two ecologically important subarctic vole species, Microtus oeconomus and Myodes rufocanus , with the two methods. DNA barcoding was conducted using the P6-loop of the chloroplast trn L (UAA) intron. Results: Although the identified plant taxa in the diets matched relatively well between the two methods, DNA barcoding gave by far taxonomically more detailed results. Quantitative comparison of results was difficult, mainly due to low taxonomic resolution of the microhistological method, which also in part explained discrepancies between the methods. Other discrepancies were likely due to biases mostly in the microhistological analysis. Conclusion: We conclude that DNA barcoding opens up for new possibilities in the study of plant-herbivore interactions, giving a detailed and relatively unbiased picture of food utilization of herbivores. Article in Journal/Newspaper Subarctic University of Tromsø: Munin Open Research Archive |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Mathematics and natural science: 400 |
spellingShingle |
VDP::Mathematics and natural science: 400 Valentini, Alice Soininen, Eeva Marjatta Coissac, Eric Ims, Rolf Anker Miquel, Christian Yoccoz, Nigel Gilles Gielly, Ludovic Brochmann, Christian Brysting, Anne K. Sønstebø, Jørn H. Taberlet, Pierre Analysing diet of small herbivores : the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures |
topic_facet |
VDP::Mathematics and natural science: 400 |
description |
Background: In order to understand the role of herbivores in trophic webs, it is essential to know what they feed on. Diet analysis is, however, a challenge in many small herbivores with a secretive life style. In this paper, we compare novel (high-throughput pyrosequencing) DNA barcoding technology for plant mixture with traditional microhistological method. We analysed stomach contents of two ecologically important subarctic vole species, Microtus oeconomus and Myodes rufocanus , with the two methods. DNA barcoding was conducted using the P6-loop of the chloroplast trn L (UAA) intron. Results: Although the identified plant taxa in the diets matched relatively well between the two methods, DNA barcoding gave by far taxonomically more detailed results. Quantitative comparison of results was difficult, mainly due to low taxonomic resolution of the microhistological method, which also in part explained discrepancies between the methods. Other discrepancies were likely due to biases mostly in the microhistological analysis. Conclusion: We conclude that DNA barcoding opens up for new possibilities in the study of plant-herbivore interactions, giving a detailed and relatively unbiased picture of food utilization of herbivores. |
format |
Article in Journal/Newspaper |
author |
Valentini, Alice Soininen, Eeva Marjatta Coissac, Eric Ims, Rolf Anker Miquel, Christian Yoccoz, Nigel Gilles Gielly, Ludovic Brochmann, Christian Brysting, Anne K. Sønstebø, Jørn H. Taberlet, Pierre |
author_facet |
Valentini, Alice Soininen, Eeva Marjatta Coissac, Eric Ims, Rolf Anker Miquel, Christian Yoccoz, Nigel Gilles Gielly, Ludovic Brochmann, Christian Brysting, Anne K. Sønstebø, Jørn H. Taberlet, Pierre |
author_sort |
Valentini, Alice |
title |
Analysing diet of small herbivores : the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures |
title_short |
Analysing diet of small herbivores : the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures |
title_full |
Analysing diet of small herbivores : the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures |
title_fullStr |
Analysing diet of small herbivores : the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures |
title_full_unstemmed |
Analysing diet of small herbivores : the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures |
title_sort |
analysing diet of small herbivores : the efficiency of dna barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures |
publisher |
BioMed Central |
publishDate |
2009 |
url |
https://hdl.handle.net/10037/2195 |
genre |
Subarctic |
genre_facet |
Subarctic |
op_relation |
Frontiers in Zoology 2009, 6:16 https://hdl.handle.net/10037/2195 URN:NBN:no-uit_munin_1947 |
op_rights |
openAccess |
_version_ |
1800739094622371840 |