Characterization of the circadian clock in Hooded Seals (Cystophora Cristata) and its interaction with mitochondrial metabolism A multi-tissue comparison and cell culture approach
Circadian rhythms regulate living organisms over a 24h period. From daily activity to cellular function. The polar regions are subject to different photoperiods across the year, which impact the environmental conditions on a seasonal and daily basis. Evidence of crosstalk between circadian core cloc...
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UiT Norges arktiske universitet
2021
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Online Access: | https://hdl.handle.net/10037/21761 |
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ftunivtroemsoe:oai:munin.uit.no:10037/21761 2023-05-15T15:15:10+02:00 Characterization of the circadian clock in Hooded Seals (Cystophora Cristata) and its interaction with mitochondrial metabolism A multi-tissue comparison and cell culture approach Kante, Fayiri 2021-06-02 https://hdl.handle.net/10037/21761 eng eng UiT Norges arktiske universitet UiT The Arctic University of Norway https://hdl.handle.net/10037/21761 Copyright 2021 The Author(s) VDP::Mathematics and natural science: 400::Zoology and botany: 480::Zoophysiology and comparative physiology: 483 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Zoofysiologi og komparativ fysiologi: 483 BIO-3950 Master thesis Mastergradsoppgave 2021 ftunivtroemsoe 2021-07-07T22:52:38Z Circadian rhythms regulate living organisms over a 24h period. From daily activity to cellular function. The polar regions are subject to different photoperiods across the year, which impact the environmental conditions on a seasonal and daily basis. Evidence of crosstalk between circadian core clock genes and hypoxia with genes involved the mitochondrial dynamics, as well as oxygen sensing, provide a basis to study those mechanisms in a model which faces those 2 challenges. The hooded seal (Cystophora Cristata) is an expert diver, with excellent hypoxia tolerance, and an arctic mammal exposed to the special light environment. The primary aim of this study was to characterize the circadian clockwork in the hooded seal using in-vivo sampling and hooded seal skin fibroblasts. The secondary aim was to explore the clockwork and the variation mitochondrial metabolism in a circadian context with the culture of hooded seal skin fibroblasts. We show that the clock genes are expressed in different seal tissues without a marked difference between midday and midnight. Nevertheless, seal skin fibroblasts respond to treatments designed to entrain the circadian clockwork and showed a significant circadian oscillation in the key clock genes; PER-2, ARNTL, NR1D1. Finally, we showed that there is a time-of-day variation in the respiratory capacity of mitochondria in seal skin fibroblasts and in MFN1, a gene involved in mitochondrial dynamics. Overall, this thesis provides new knowledge on the circadian rhythm in seals and how mitochondrial metabolism may be influenced. These data encourage further research to depict mitochondrial dynamics and metabolism in a circadian and hypoxic conditions, as well as animal circadian behavior for an integrative perspective. Master Thesis Arctic Cystophora cristata hooded seal University of Tromsø: Munin Open Research Archive Arctic |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Mathematics and natural science: 400::Zoology and botany: 480::Zoophysiology and comparative physiology: 483 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Zoofysiologi og komparativ fysiologi: 483 BIO-3950 |
spellingShingle |
VDP::Mathematics and natural science: 400::Zoology and botany: 480::Zoophysiology and comparative physiology: 483 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Zoofysiologi og komparativ fysiologi: 483 BIO-3950 Kante, Fayiri Characterization of the circadian clock in Hooded Seals (Cystophora Cristata) and its interaction with mitochondrial metabolism A multi-tissue comparison and cell culture approach |
topic_facet |
VDP::Mathematics and natural science: 400::Zoology and botany: 480::Zoophysiology and comparative physiology: 483 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Zoofysiologi og komparativ fysiologi: 483 BIO-3950 |
description |
Circadian rhythms regulate living organisms over a 24h period. From daily activity to cellular function. The polar regions are subject to different photoperiods across the year, which impact the environmental conditions on a seasonal and daily basis. Evidence of crosstalk between circadian core clock genes and hypoxia with genes involved the mitochondrial dynamics, as well as oxygen sensing, provide a basis to study those mechanisms in a model which faces those 2 challenges. The hooded seal (Cystophora Cristata) is an expert diver, with excellent hypoxia tolerance, and an arctic mammal exposed to the special light environment. The primary aim of this study was to characterize the circadian clockwork in the hooded seal using in-vivo sampling and hooded seal skin fibroblasts. The secondary aim was to explore the clockwork and the variation mitochondrial metabolism in a circadian context with the culture of hooded seal skin fibroblasts. We show that the clock genes are expressed in different seal tissues without a marked difference between midday and midnight. Nevertheless, seal skin fibroblasts respond to treatments designed to entrain the circadian clockwork and showed a significant circadian oscillation in the key clock genes; PER-2, ARNTL, NR1D1. Finally, we showed that there is a time-of-day variation in the respiratory capacity of mitochondria in seal skin fibroblasts and in MFN1, a gene involved in mitochondrial dynamics. Overall, this thesis provides new knowledge on the circadian rhythm in seals and how mitochondrial metabolism may be influenced. These data encourage further research to depict mitochondrial dynamics and metabolism in a circadian and hypoxic conditions, as well as animal circadian behavior for an integrative perspective. |
format |
Master Thesis |
author |
Kante, Fayiri |
author_facet |
Kante, Fayiri |
author_sort |
Kante, Fayiri |
title |
Characterization of the circadian clock in Hooded Seals (Cystophora Cristata) and its interaction with mitochondrial metabolism A multi-tissue comparison and cell culture approach |
title_short |
Characterization of the circadian clock in Hooded Seals (Cystophora Cristata) and its interaction with mitochondrial metabolism A multi-tissue comparison and cell culture approach |
title_full |
Characterization of the circadian clock in Hooded Seals (Cystophora Cristata) and its interaction with mitochondrial metabolism A multi-tissue comparison and cell culture approach |
title_fullStr |
Characterization of the circadian clock in Hooded Seals (Cystophora Cristata) and its interaction with mitochondrial metabolism A multi-tissue comparison and cell culture approach |
title_full_unstemmed |
Characterization of the circadian clock in Hooded Seals (Cystophora Cristata) and its interaction with mitochondrial metabolism A multi-tissue comparison and cell culture approach |
title_sort |
characterization of the circadian clock in hooded seals (cystophora cristata) and its interaction with mitochondrial metabolism a multi-tissue comparison and cell culture approach |
publisher |
UiT Norges arktiske universitet |
publishDate |
2021 |
url |
https://hdl.handle.net/10037/21761 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Cystophora cristata hooded seal |
genre_facet |
Arctic Cystophora cristata hooded seal |
op_relation |
https://hdl.handle.net/10037/21761 |
op_rights |
Copyright 2021 The Author(s) |
_version_ |
1766345541127503872 |