Photoperiod-dependent developmental reprogramming of the transcriptional response to seawater entry in Atlantic salmon (Salmo salar)
The developmental transition of juvenile salmon from a freshwater resident morph (parr) to a seawater (SW) migratory morph (smolt), known as smoltification, entails a reorganization of gill function to cope with the altered water environment. Recently, we used RNAseq to characterize the breadth of t...
Published in: | G3 Genes|Genomes|Genetics |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Oxford University Press
2021
|
Subjects: | |
Online Access: | https://hdl.handle.net/10037/21731 https://doi.org/10.1093/g3journal/jkab072 |
id |
ftunivtroemsoe:oai:munin.uit.no:10037/21731 |
---|---|
record_format |
openpolar |
spelling |
ftunivtroemsoe:oai:munin.uit.no:10037/21731 2023-05-15T15:32:54+02:00 Photoperiod-dependent developmental reprogramming of the transcriptional response to seawater entry in Atlantic salmon (Salmo salar) Iversen, Marianne Mulugeta, Teshome Dagne West, Alexander Christopher Jørgensen, Even Hjalmar Martin, Samuel A. M. Sandve, Simen Rød Hazlerigg, David 2021-03-12 https://hdl.handle.net/10037/21731 https://doi.org/10.1093/g3journal/jkab072 eng eng Oxford University Press G3: Genes, Genomes, Genetics Norges forskningsråd: 241016 info:eu-repo/grantAgreement/RCN/FRIMEDBIO/241016/Norway/Light & Salt - Thyroid hormone deiodinase paralogues & the evolution of complex life-history strategy in salmonids// Iversen, Mulugeta, West, Jørgensen, Martin, Sandve, Hazlerigg. Photoperiod-dependent developmental reprogramming of the transcriptional response to seawater entry in Atlantic salmon (Salmo salar). G3: Genes, Genomes, Genetics. 2021;11(4) FRIDAID 1919152 doi:10.1093/g3journal/jkab072 2160-1836 https://hdl.handle.net/10037/21731 openAccess Copyright 2021 The Author(s) VDP::Mathematics and natural science: 400::Information and communication science: 420 VDP::Matematikk og Naturvitenskap: 400::Informasjons- og kommunikasjonsvitenskap: 420 Journal article Tidsskriftartikkel Peer reviewed publishedVersion 2021 ftunivtroemsoe https://doi.org/10.1093/g3journal/jkab072 2021-07-07T22:52:38Z The developmental transition of juvenile salmon from a freshwater resident morph (parr) to a seawater (SW) migratory morph (smolt), known as smoltification, entails a reorganization of gill function to cope with the altered water environment. Recently, we used RNAseq to characterize the breadth of transcriptional change which takes place in the gill in the FW phase of smoltification. This highlighted the importance of extended exposure to short, winter-like photoperiods (SP) followed by a subsequent increase in photoperiod for completion of transcriptional reprogramming in FW and efficient growth following transfer to SW. Here, we extend this analysis to examine the consequences of this photoperiodic history-dependent reprogramming for subsequent gill responses upon exposure to SW. We use RNAseq to analyze gill samples taken from fish raised on the photoperiod regimes we used previously and then challenged by SW exposure for 24 hours. While fish held on constant light (LL) throughout were able to hypo-osmoregulate during a 24 hours SW challenge, the associated gill transcriptional response was highly distinctive from that in fish which had experienced a 7-week period of exposure to SP followed by a return to LL (SPLL) and had consequently acquired the characteristics of fully developed smolts. Fish transferred from LL to SP, and then held on SP for the remainder of the study was unable to hypo-osmoregulate, and the associated gill transcriptional response to SW exposure featured many transcripts apparently regulated by the glucocorticoid stress axis and by the osmo-sensing transcription factor NFAT5. The importance of these pathways for the gill transcriptional response to SW exposure appears to diminish as a consequence of photoperiod mediated induction of the smolt phenotype, presumably reflecting preparatory developmental changes taking place during this process. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Tromsø: Munin Open Research Archive G3 Genes|Genomes|Genetics 11 4 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Mathematics and natural science: 400::Information and communication science: 420 VDP::Matematikk og Naturvitenskap: 400::Informasjons- og kommunikasjonsvitenskap: 420 |
spellingShingle |
VDP::Mathematics and natural science: 400::Information and communication science: 420 VDP::Matematikk og Naturvitenskap: 400::Informasjons- og kommunikasjonsvitenskap: 420 Iversen, Marianne Mulugeta, Teshome Dagne West, Alexander Christopher Jørgensen, Even Hjalmar Martin, Samuel A. M. Sandve, Simen Rød Hazlerigg, David Photoperiod-dependent developmental reprogramming of the transcriptional response to seawater entry in Atlantic salmon (Salmo salar) |
topic_facet |
VDP::Mathematics and natural science: 400::Information and communication science: 420 VDP::Matematikk og Naturvitenskap: 400::Informasjons- og kommunikasjonsvitenskap: 420 |
description |
The developmental transition of juvenile salmon from a freshwater resident morph (parr) to a seawater (SW) migratory morph (smolt), known as smoltification, entails a reorganization of gill function to cope with the altered water environment. Recently, we used RNAseq to characterize the breadth of transcriptional change which takes place in the gill in the FW phase of smoltification. This highlighted the importance of extended exposure to short, winter-like photoperiods (SP) followed by a subsequent increase in photoperiod for completion of transcriptional reprogramming in FW and efficient growth following transfer to SW. Here, we extend this analysis to examine the consequences of this photoperiodic history-dependent reprogramming for subsequent gill responses upon exposure to SW. We use RNAseq to analyze gill samples taken from fish raised on the photoperiod regimes we used previously and then challenged by SW exposure for 24 hours. While fish held on constant light (LL) throughout were able to hypo-osmoregulate during a 24 hours SW challenge, the associated gill transcriptional response was highly distinctive from that in fish which had experienced a 7-week period of exposure to SP followed by a return to LL (SPLL) and had consequently acquired the characteristics of fully developed smolts. Fish transferred from LL to SP, and then held on SP for the remainder of the study was unable to hypo-osmoregulate, and the associated gill transcriptional response to SW exposure featured many transcripts apparently regulated by the glucocorticoid stress axis and by the osmo-sensing transcription factor NFAT5. The importance of these pathways for the gill transcriptional response to SW exposure appears to diminish as a consequence of photoperiod mediated induction of the smolt phenotype, presumably reflecting preparatory developmental changes taking place during this process. |
format |
Article in Journal/Newspaper |
author |
Iversen, Marianne Mulugeta, Teshome Dagne West, Alexander Christopher Jørgensen, Even Hjalmar Martin, Samuel A. M. Sandve, Simen Rød Hazlerigg, David |
author_facet |
Iversen, Marianne Mulugeta, Teshome Dagne West, Alexander Christopher Jørgensen, Even Hjalmar Martin, Samuel A. M. Sandve, Simen Rød Hazlerigg, David |
author_sort |
Iversen, Marianne |
title |
Photoperiod-dependent developmental reprogramming of the transcriptional response to seawater entry in Atlantic salmon (Salmo salar) |
title_short |
Photoperiod-dependent developmental reprogramming of the transcriptional response to seawater entry in Atlantic salmon (Salmo salar) |
title_full |
Photoperiod-dependent developmental reprogramming of the transcriptional response to seawater entry in Atlantic salmon (Salmo salar) |
title_fullStr |
Photoperiod-dependent developmental reprogramming of the transcriptional response to seawater entry in Atlantic salmon (Salmo salar) |
title_full_unstemmed |
Photoperiod-dependent developmental reprogramming of the transcriptional response to seawater entry in Atlantic salmon (Salmo salar) |
title_sort |
photoperiod-dependent developmental reprogramming of the transcriptional response to seawater entry in atlantic salmon (salmo salar) |
publisher |
Oxford University Press |
publishDate |
2021 |
url |
https://hdl.handle.net/10037/21731 https://doi.org/10.1093/g3journal/jkab072 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
G3: Genes, Genomes, Genetics Norges forskningsråd: 241016 info:eu-repo/grantAgreement/RCN/FRIMEDBIO/241016/Norway/Light & Salt - Thyroid hormone deiodinase paralogues & the evolution of complex life-history strategy in salmonids// Iversen, Mulugeta, West, Jørgensen, Martin, Sandve, Hazlerigg. Photoperiod-dependent developmental reprogramming of the transcriptional response to seawater entry in Atlantic salmon (Salmo salar). G3: Genes, Genomes, Genetics. 2021;11(4) FRIDAID 1919152 doi:10.1093/g3journal/jkab072 2160-1836 https://hdl.handle.net/10037/21731 |
op_rights |
openAccess Copyright 2021 The Author(s) |
op_doi |
https://doi.org/10.1093/g3journal/jkab072 |
container_title |
G3 Genes|Genomes|Genetics |
container_volume |
11 |
container_issue |
4 |
_version_ |
1766363384829181952 |