The occurrence of Nematoda in coastal sea ice on Svalbard (European Arctic) determined with the 18S small subunit rRNA gene
Understanding the diversity and functioning of Arctic sea ice ecosystems is vital to evaluate and predict the impact of current and future climate change. In the microscopic communities inhabiting the brine channels inside sea ice, nematodes often dominate numerically and act as bacterivores and her...
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Online Access: | https://hdl.handle.net/10037/21667 https://doi.org/10.1007/s00300-021-02863-y |
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ftunivtroemsoe:oai:munin.uit.no:10037/21667 2023-05-15T14:25:30+02:00 The occurrence of Nematoda in coastal sea ice on Svalbard (European Arctic) determined with the 18S small subunit rRNA gene Pitusi, Vanessa Søreide, Janne Hassett, Brandon Marquardt, Miriam Andreasen, Magnus Heide 2021-05-06 https://hdl.handle.net/10037/21667 https://doi.org/10.1007/s00300-021-02863-y eng eng Springer Polar Biology Pitusi, Søreide, Hassett BT, Marquardt, Andreasen. The occurrence of Nematoda in coastal sea ice on Svalbard (European Arctic) determined with the 18S small subunit rRNA gene. Polar Biology. 2021;44:1153-1162 FRIDAID 1917647 doi:10.1007/s00300-021-02863-y 0722-4060 1432-2056 https://hdl.handle.net/10037/21667 Copyright 2021 The Author(s) VDP::Mathematics and natural science: 400::Zoology and botany: 480 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480 Journal article Tidsskriftartikkel Peer reviewed publishedVersion 2021 ftunivtroemsoe https://doi.org/10.1007/s00300-021-02863-y 2021-07-07T22:52:38Z Understanding the diversity and functioning of Arctic sea ice ecosystems is vital to evaluate and predict the impact of current and future climate change. In the microscopic communities inhabiting the brine channels inside sea ice, nematodes often dominate numerically and act as bacterivores and herbivores. Despite nematodes great abundances and known ecological roles, molecular tools have not been applied to investigate their species diversity in sea ice. In an attempt to begin establishing a molecular baseline for species diversity of sea ice nematodes, we Sanger sequenced 74 specimens from four locations around Svalbard (European Arctic), using the 18S rRNA barcode. Currently available nucleotide reference databases are both underpopulated with representative marine nematode taxa and contain a substantial number of misidentified organisms. Together, these limitations inhibited the ability to identify marine specimens collected in this study with certainty. Nevertheless, our molecular data indicate the presence of two genera in sea ice on Svalbard—Theristus and Halomonhystera. While it is possible that the latter represents a novel ice nematode species, future studies, including morphometric analysis, are needed to verify our molecular findings. We leverage the assignment of molecular information to robustly identify nematodes and provide the first insight into the diversity of sea ice nematodes in the European Arctic. We advocate for an intensified cooperation between molecular and morphological taxonomists to expedite the establishment of baseline surveys that are required to predict biological consequences of the diminishing sea ice habitat in the future. Article in Journal/Newspaper Arctic Arctic Climate change Polar Biology Sea ice Svalbard University of Tromsø: Munin Open Research Archive Arctic Svalbard Polar Biology 44 6 1153 1162 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Mathematics and natural science: 400::Zoology and botany: 480 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480 |
spellingShingle |
VDP::Mathematics and natural science: 400::Zoology and botany: 480 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480 Pitusi, Vanessa Søreide, Janne Hassett, Brandon Marquardt, Miriam Andreasen, Magnus Heide The occurrence of Nematoda in coastal sea ice on Svalbard (European Arctic) determined with the 18S small subunit rRNA gene |
topic_facet |
VDP::Mathematics and natural science: 400::Zoology and botany: 480 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480 |
description |
Understanding the diversity and functioning of Arctic sea ice ecosystems is vital to evaluate and predict the impact of current and future climate change. In the microscopic communities inhabiting the brine channels inside sea ice, nematodes often dominate numerically and act as bacterivores and herbivores. Despite nematodes great abundances and known ecological roles, molecular tools have not been applied to investigate their species diversity in sea ice. In an attempt to begin establishing a molecular baseline for species diversity of sea ice nematodes, we Sanger sequenced 74 specimens from four locations around Svalbard (European Arctic), using the 18S rRNA barcode. Currently available nucleotide reference databases are both underpopulated with representative marine nematode taxa and contain a substantial number of misidentified organisms. Together, these limitations inhibited the ability to identify marine specimens collected in this study with certainty. Nevertheless, our molecular data indicate the presence of two genera in sea ice on Svalbard—Theristus and Halomonhystera. While it is possible that the latter represents a novel ice nematode species, future studies, including morphometric analysis, are needed to verify our molecular findings. We leverage the assignment of molecular information to robustly identify nematodes and provide the first insight into the diversity of sea ice nematodes in the European Arctic. We advocate for an intensified cooperation between molecular and morphological taxonomists to expedite the establishment of baseline surveys that are required to predict biological consequences of the diminishing sea ice habitat in the future. |
format |
Article in Journal/Newspaper |
author |
Pitusi, Vanessa Søreide, Janne Hassett, Brandon Marquardt, Miriam Andreasen, Magnus Heide |
author_facet |
Pitusi, Vanessa Søreide, Janne Hassett, Brandon Marquardt, Miriam Andreasen, Magnus Heide |
author_sort |
Pitusi, Vanessa |
title |
The occurrence of Nematoda in coastal sea ice on Svalbard (European Arctic) determined with the 18S small subunit rRNA gene |
title_short |
The occurrence of Nematoda in coastal sea ice on Svalbard (European Arctic) determined with the 18S small subunit rRNA gene |
title_full |
The occurrence of Nematoda in coastal sea ice on Svalbard (European Arctic) determined with the 18S small subunit rRNA gene |
title_fullStr |
The occurrence of Nematoda in coastal sea ice on Svalbard (European Arctic) determined with the 18S small subunit rRNA gene |
title_full_unstemmed |
The occurrence of Nematoda in coastal sea ice on Svalbard (European Arctic) determined with the 18S small subunit rRNA gene |
title_sort |
occurrence of nematoda in coastal sea ice on svalbard (european arctic) determined with the 18s small subunit rrna gene |
publisher |
Springer |
publishDate |
2021 |
url |
https://hdl.handle.net/10037/21667 https://doi.org/10.1007/s00300-021-02863-y |
geographic |
Arctic Svalbard |
geographic_facet |
Arctic Svalbard |
genre |
Arctic Arctic Climate change Polar Biology Sea ice Svalbard |
genre_facet |
Arctic Arctic Climate change Polar Biology Sea ice Svalbard |
op_relation |
Polar Biology Pitusi, Søreide, Hassett BT, Marquardt, Andreasen. The occurrence of Nematoda in coastal sea ice on Svalbard (European Arctic) determined with the 18S small subunit rRNA gene. Polar Biology. 2021;44:1153-1162 FRIDAID 1917647 doi:10.1007/s00300-021-02863-y 0722-4060 1432-2056 https://hdl.handle.net/10037/21667 |
op_rights |
Copyright 2021 The Author(s) |
op_doi |
https://doi.org/10.1007/s00300-021-02863-y |
container_title |
Polar Biology |
container_volume |
44 |
container_issue |
6 |
container_start_page |
1153 |
op_container_end_page |
1162 |
_version_ |
1766297887537364992 |