Effectiveness of Fluorescent Viability Assays in Studies of Arctic Cold Seep Foraminifera
Highly negative δ 13 C values in fossil foraminifera from methane cold seeps have been proposed to reflect episodes of methane release from gas hydrate dissociation or free gas reservoirs triggered by climatic changes in the past. Because most studies on live foraminifera are based on the presence o...
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Online Access: | https://hdl.handle.net/10037/21033 https://doi.org/10.3389/fmars.2021.587748 |
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ftunivtroemsoe:oai:munin.uit.no:10037/21033 2023-05-15T14:22:16+02:00 Effectiveness of Fluorescent Viability Assays in Studies of Arctic Cold Seep Foraminifera Melaniuk, Katarzyna 2021-03-09 https://hdl.handle.net/10037/21033 https://doi.org/10.3389/fmars.2021.587748 eng eng Frontiers Media Melaniuk, K. (2021). Assessing the relationship between living benthic foraminifera and methane emission in the Arctic Ocean. (Doctoral thesis). https://hdl.handle.net/10037/22757 . Frontiers in Marine Science info:eu-repo/grantAgreement/RCN/SFF/223259/Norway/Centre for Arctic Gas Hydrate, Environment and Climate/CAGE/ Melaniuk K. Effectiveness of Fluorescent Viability Assays in Studies of Arctic Cold Seep Foraminifera. Frontiers in Marine Science. 2021;8:1-21 FRIDAID 1897652 doi:10.3389/fmars.2021.587748 2296-7745 https://hdl.handle.net/10037/21033 openAccess Copyright 2021 The Author(s) VDP::Mathematics and natural science: 400::Geosciences: 450::Stratigraphy and paleontology: 461 VDP::Matematikk og Naturvitenskap: 400::Geofag: 450::Stratigrafi og paleontologi: 461 Journal article Tidsskriftartikkel Peer reviewed publishedVersion 2021 ftunivtroemsoe https://doi.org/10.3389/fmars.2021.587748 2021-10-13T22:53:55Z Highly negative δ 13 C values in fossil foraminifera from methane cold seeps have been proposed to reflect episodes of methane release from gas hydrate dissociation or free gas reservoirs triggered by climatic changes in the past. Because most studies on live foraminifera are based on the presence of Rose Bengal staining, that colors the cytoplasm of both live and recently dead individuals it remains unclear if, and to what extent live foraminifera incorporate methane-derived carbon during biomineralization, or whether the isotopic signature is mostly affected by authigenic overgrowth. In this paper, modern foraminiferal assemblages from a gas hydrate province Vestnesa Ridge (∼1,200 m water depth, northeastern Fram Strait) and from Storfjordrenna (∼400 m water depth in the western Barents Sea) is presented. By using the fluorescent viability assays CellTracker TM Green (CTG) CMFDA and CellHunt Green (CHG) together with conventional Rose Bengal, it was possible to examine live and recently dead foraminifera separately. Metabolically active foraminifera were shown to inhabit methane-enriched sediments at both investigated locations. The benthic foraminiferal faunas were dominated by common Arctic species such as Melonis barleeanus, Cassidulina neoteretis, and Nonionellina labradorica. The combined usage of the fluorescence probe and Rose Bengal revealed only minor shifts in species compositions and differences in ratios between live and recently dead foraminifera from Storfjordrenna. There was no clear evidence that methane significantly affected the δ 13 C signature of the calcite of living specimens. Article in Journal/Newspaper Arctic Arctic Barents Sea Foraminifera* Fram Strait Storfjordrenna University of Tromsø: Munin Open Research Archive Arctic Barents Sea Storfjordrenna ENVELOPE(17.000,17.000,76.000,76.000) Frontiers in Marine Science 8 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Mathematics and natural science: 400::Geosciences: 450::Stratigraphy and paleontology: 461 VDP::Matematikk og Naturvitenskap: 400::Geofag: 450::Stratigrafi og paleontologi: 461 |
spellingShingle |
VDP::Mathematics and natural science: 400::Geosciences: 450::Stratigraphy and paleontology: 461 VDP::Matematikk og Naturvitenskap: 400::Geofag: 450::Stratigrafi og paleontologi: 461 Melaniuk, Katarzyna Effectiveness of Fluorescent Viability Assays in Studies of Arctic Cold Seep Foraminifera |
topic_facet |
VDP::Mathematics and natural science: 400::Geosciences: 450::Stratigraphy and paleontology: 461 VDP::Matematikk og Naturvitenskap: 400::Geofag: 450::Stratigrafi og paleontologi: 461 |
description |
Highly negative δ 13 C values in fossil foraminifera from methane cold seeps have been proposed to reflect episodes of methane release from gas hydrate dissociation or free gas reservoirs triggered by climatic changes in the past. Because most studies on live foraminifera are based on the presence of Rose Bengal staining, that colors the cytoplasm of both live and recently dead individuals it remains unclear if, and to what extent live foraminifera incorporate methane-derived carbon during biomineralization, or whether the isotopic signature is mostly affected by authigenic overgrowth. In this paper, modern foraminiferal assemblages from a gas hydrate province Vestnesa Ridge (∼1,200 m water depth, northeastern Fram Strait) and from Storfjordrenna (∼400 m water depth in the western Barents Sea) is presented. By using the fluorescent viability assays CellTracker TM Green (CTG) CMFDA and CellHunt Green (CHG) together with conventional Rose Bengal, it was possible to examine live and recently dead foraminifera separately. Metabolically active foraminifera were shown to inhabit methane-enriched sediments at both investigated locations. The benthic foraminiferal faunas were dominated by common Arctic species such as Melonis barleeanus, Cassidulina neoteretis, and Nonionellina labradorica. The combined usage of the fluorescence probe and Rose Bengal revealed only minor shifts in species compositions and differences in ratios between live and recently dead foraminifera from Storfjordrenna. There was no clear evidence that methane significantly affected the δ 13 C signature of the calcite of living specimens. |
format |
Article in Journal/Newspaper |
author |
Melaniuk, Katarzyna |
author_facet |
Melaniuk, Katarzyna |
author_sort |
Melaniuk, Katarzyna |
title |
Effectiveness of Fluorescent Viability Assays in Studies of Arctic Cold Seep Foraminifera |
title_short |
Effectiveness of Fluorescent Viability Assays in Studies of Arctic Cold Seep Foraminifera |
title_full |
Effectiveness of Fluorescent Viability Assays in Studies of Arctic Cold Seep Foraminifera |
title_fullStr |
Effectiveness of Fluorescent Viability Assays in Studies of Arctic Cold Seep Foraminifera |
title_full_unstemmed |
Effectiveness of Fluorescent Viability Assays in Studies of Arctic Cold Seep Foraminifera |
title_sort |
effectiveness of fluorescent viability assays in studies of arctic cold seep foraminifera |
publisher |
Frontiers Media |
publishDate |
2021 |
url |
https://hdl.handle.net/10037/21033 https://doi.org/10.3389/fmars.2021.587748 |
long_lat |
ENVELOPE(17.000,17.000,76.000,76.000) |
geographic |
Arctic Barents Sea Storfjordrenna |
geographic_facet |
Arctic Barents Sea Storfjordrenna |
genre |
Arctic Arctic Barents Sea Foraminifera* Fram Strait Storfjordrenna |
genre_facet |
Arctic Arctic Barents Sea Foraminifera* Fram Strait Storfjordrenna |
op_relation |
Melaniuk, K. (2021). Assessing the relationship between living benthic foraminifera and methane emission in the Arctic Ocean. (Doctoral thesis). https://hdl.handle.net/10037/22757 . Frontiers in Marine Science info:eu-repo/grantAgreement/RCN/SFF/223259/Norway/Centre for Arctic Gas Hydrate, Environment and Climate/CAGE/ Melaniuk K. Effectiveness of Fluorescent Viability Assays in Studies of Arctic Cold Seep Foraminifera. Frontiers in Marine Science. 2021;8:1-21 FRIDAID 1897652 doi:10.3389/fmars.2021.587748 2296-7745 https://hdl.handle.net/10037/21033 |
op_rights |
openAccess Copyright 2021 The Author(s) |
op_doi |
https://doi.org/10.3389/fmars.2021.587748 |
container_title |
Frontiers in Marine Science |
container_volume |
8 |
_version_ |
1766294912705232896 |