Effects of dietary soybean meal, inulin and oxytetracycline on gastrointestinal histological characteristics, distal intestine cell proliferation and intestinal microbiota in Atlantic salmon (Salmo salar L.)
This is the publishers version/PDF (institutional repository or PubMed Central can publish publishers version after 12 month embargo) Soyabean meal (SBM)-induced enteritis in the distal intestine of the teleost Atlantic salmon (Salmo salar L.) and other salmonids may be considered a model for diet-r...
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Online Access: | https://hdl.handle.net/10037/2103 |
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ftunivtroemsoe:oai:munin.uit.no:10037/2103 2024-06-02T08:03:38+00:00 Effects of dietary soybean meal, inulin and oxytetracycline on gastrointestinal histological characteristics, distal intestine cell proliferation and intestinal microbiota in Atlantic salmon (Salmo salar L.) Bakke-Mckellep, AM Sperstad, Sigmund Penn, MH Salas, PM Refstie, Ståle Landsverk, Tor Ringø, Einar Krogdahl, Åshild 2007 2478869 bytes application/pdf https://hdl.handle.net/10037/2103 eng eng Cambridge University Press British Journal of Nutrition 97(2007) s. 699-713 DOI:10.1017/S0007114507381397 FRIDAID 393604 0007-1145 https://hdl.handle.net/10037/2103 URN:NBN:no-uit_munin_1854 openAccess VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923 Inflammation Enteritis Mucosal disorder Diet Intestine Journal article Tidsskriftartikkel Peer reviewed 2007 ftunivtroemsoe https://doi.org/10.1017/S0007114507381397 2024-05-07T08:41:49Z This is the publishers version/PDF (institutional repository or PubMed Central can publish publishers version after 12 month embargo) Soyabean meal (SBM)-induced enteritis in the distal intestine of the teleost Atlantic salmon (Salmo salar L.) and other salmonids may be considered a model for diet-related mucosal disorders in other animals and man. The role of the intestinal microbiota in its pathogenesis was explored. Compared to diets containing fishmeal (FM) as the sole protein source, responses to extracted SBM or the prebiotic inulin, with or without oxytetracycline (OTC) inclusion, were studied following a 3-week feeding trial. Intestinal microbiota, organosomatic indices and histology, as well as immunohistochemical detection of proliferating cell nuclear antigen (PCNA), heat shock protein 70 (HSP70) and caspase-3-positive cells in the distal intestine, were studied. Distal intestine somatic indices (DISI) were higher in inulin and lower in SBM compared to FM-fed fish. The low DISI caused by SBM corresponded with histological changes, neither of which was affected by OTC, despite a significant decrease in adherent bacteria count. Image analysis of PCNA-stained sections showed a significant increase in the proliferative compartment length in SBM-fed fish, accompanied by apparent increases in reactivity to HSP70 and caspase-3 along the mucosal folds, indicating induction of cellular repair and apoptosis, respectively. Fish fed the SBM diet had higher total number as well as a more diverse population composition of adherent bacteria in the distal intestine. Thus SBM-induced enteritis is accompanied by induction of distal intestinal epithelial cell protective responses and changes in microbiota. Putative involvement of bacteria in the inflammatory response merits further investigation. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Tromsø: Munin Open Research Archive British Journal of Nutrition 97 4 699 713 |
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Open Polar |
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University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923 Inflammation Enteritis Mucosal disorder Diet Intestine |
spellingShingle |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923 Inflammation Enteritis Mucosal disorder Diet Intestine Bakke-Mckellep, AM Sperstad, Sigmund Penn, MH Salas, PM Refstie, Ståle Landsverk, Tor Ringø, Einar Krogdahl, Åshild Effects of dietary soybean meal, inulin and oxytetracycline on gastrointestinal histological characteristics, distal intestine cell proliferation and intestinal microbiota in Atlantic salmon (Salmo salar L.) |
topic_facet |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923 Inflammation Enteritis Mucosal disorder Diet Intestine |
description |
This is the publishers version/PDF (institutional repository or PubMed Central can publish publishers version after 12 month embargo) Soyabean meal (SBM)-induced enteritis in the distal intestine of the teleost Atlantic salmon (Salmo salar L.) and other salmonids may be considered a model for diet-related mucosal disorders in other animals and man. The role of the intestinal microbiota in its pathogenesis was explored. Compared to diets containing fishmeal (FM) as the sole protein source, responses to extracted SBM or the prebiotic inulin, with or without oxytetracycline (OTC) inclusion, were studied following a 3-week feeding trial. Intestinal microbiota, organosomatic indices and histology, as well as immunohistochemical detection of proliferating cell nuclear antigen (PCNA), heat shock protein 70 (HSP70) and caspase-3-positive cells in the distal intestine, were studied. Distal intestine somatic indices (DISI) were higher in inulin and lower in SBM compared to FM-fed fish. The low DISI caused by SBM corresponded with histological changes, neither of which was affected by OTC, despite a significant decrease in adherent bacteria count. Image analysis of PCNA-stained sections showed a significant increase in the proliferative compartment length in SBM-fed fish, accompanied by apparent increases in reactivity to HSP70 and caspase-3 along the mucosal folds, indicating induction of cellular repair and apoptosis, respectively. Fish fed the SBM diet had higher total number as well as a more diverse population composition of adherent bacteria in the distal intestine. Thus SBM-induced enteritis is accompanied by induction of distal intestinal epithelial cell protective responses and changes in microbiota. Putative involvement of bacteria in the inflammatory response merits further investigation. |
format |
Article in Journal/Newspaper |
author |
Bakke-Mckellep, AM Sperstad, Sigmund Penn, MH Salas, PM Refstie, Ståle Landsverk, Tor Ringø, Einar Krogdahl, Åshild |
author_facet |
Bakke-Mckellep, AM Sperstad, Sigmund Penn, MH Salas, PM Refstie, Ståle Landsverk, Tor Ringø, Einar Krogdahl, Åshild |
author_sort |
Bakke-Mckellep, AM |
title |
Effects of dietary soybean meal, inulin and oxytetracycline on gastrointestinal histological characteristics, distal intestine cell proliferation and intestinal microbiota in Atlantic salmon (Salmo salar L.) |
title_short |
Effects of dietary soybean meal, inulin and oxytetracycline on gastrointestinal histological characteristics, distal intestine cell proliferation and intestinal microbiota in Atlantic salmon (Salmo salar L.) |
title_full |
Effects of dietary soybean meal, inulin and oxytetracycline on gastrointestinal histological characteristics, distal intestine cell proliferation and intestinal microbiota in Atlantic salmon (Salmo salar L.) |
title_fullStr |
Effects of dietary soybean meal, inulin and oxytetracycline on gastrointestinal histological characteristics, distal intestine cell proliferation and intestinal microbiota in Atlantic salmon (Salmo salar L.) |
title_full_unstemmed |
Effects of dietary soybean meal, inulin and oxytetracycline on gastrointestinal histological characteristics, distal intestine cell proliferation and intestinal microbiota in Atlantic salmon (Salmo salar L.) |
title_sort |
effects of dietary soybean meal, inulin and oxytetracycline on gastrointestinal histological characteristics, distal intestine cell proliferation and intestinal microbiota in atlantic salmon (salmo salar l.) |
publisher |
Cambridge University Press |
publishDate |
2007 |
url |
https://hdl.handle.net/10037/2103 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
British Journal of Nutrition 97(2007) s. 699-713 DOI:10.1017/S0007114507381397 FRIDAID 393604 0007-1145 https://hdl.handle.net/10037/2103 URN:NBN:no-uit_munin_1854 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1017/S0007114507381397 |
container_title |
British Journal of Nutrition |
container_volume |
97 |
container_issue |
4 |
container_start_page |
699 |
op_container_end_page |
713 |
_version_ |
1800748210595037184 |