Pathogen surveillance in Southern Ocean pinnipeds
Knowledge of the health status and potential effect of disease outbreaks among Southern Ocean fauna may be decisive for its conservation. We assessed the exposure and infection of Antarctic fur seals ( Arctocephalus gazella , AFS) and Southern elephant seals ( Mirounga leonine , SES) to parapoxvirus...
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Norsk Polarinstitutt
2020
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Online Access: | https://hdl.handle.net/10037/20722 https://doi.org/10.33265/polar.v39.3841 |
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ftunivtroemsoe:oai:munin.uit.no:10037/20722 2023-05-15T13:52:48+02:00 Pathogen surveillance in Southern Ocean pinnipeds Núñez-Egido, Sandra Lowther, Andrew Nymo, Ingebørg H. Klein, Jörn Breines, Eva Marie Tryland, Morten 2020-12-17 https://hdl.handle.net/10037/20722 https://doi.org/10.33265/polar.v39.3841 eng eng Norsk Polarinstitutt Polar Research Núñez-Egido, Lowther, Nymo, Klein, Breines EM, Tryland M. Pathogen surveillance in Southern Ocean pinnipeds. Polar Research. 2020;39:1-11 FRIDAID 1865797 doi:10.33265/polar.v39.3841 0800-0395 1751-8369 https://hdl.handle.net/10037/20722 openAccess Copyright 2020 The Author(s) Dyrehelse / Animal health Sel / Pinnipeds VDP::Mathematics and natural science: 400::Zoology and botany: 480 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480 Journal article Tidsskriftartikkel Peer reviewed publishedVersion 2020 ftunivtroemsoe https://doi.org/10.33265/polar.v39.3841 2021-06-25T17:57:56Z Knowledge of the health status and potential effect of disease outbreaks among Southern Ocean fauna may be decisive for its conservation. We assessed the exposure and infection of Antarctic fur seals ( Arctocephalus gazella , AFS) and Southern elephant seals ( Mirounga leonine , SES) to parapoxvirus, Phocid alphaherpesvirus-1 (PhHV-1), smooth Brucella spp. and Toxoplasma gondii . AFS ( n = 65) serum and swab samples, and SES ( n = 13) serum samples from the sub--Antarctic island of Bouvetøya (54°25’S, 03°22’E) were collected during two austral summers (2014/15, 2017/18). Three polymerase chain reaction (PCR) tests amplifying the DNA polymerase, B2L and GIF parapoxvirus genomic regions were performed, investigating DNA from mucosal swab samples. The glycoprotein B gene was targeted to detect PhHV-1 viral DNA. Sera were assayed for T. gondii and smooth Brucella spp. antibodies with indirect enzyme-linked immunosorbent assays. Parapoxvirus PCR amplicons of the expected size were generated in two of the 29 AFS pups (nasal swabs, 2014/15), targeting the B2L ( n = 2) and DNA polymerase ( n = 1) genes, whereas the GIF PCR did not amplify target sequences. The PCR amplicons were sequenced and blasted in GenBank, revealing highest similarity with a seal parapoxvirus, confirming the presence of the virus in AFS for the first time. No PhHV-1 amplicons were generated, and antibodies against T. gondii or smooth Brucella spp. were not detected. Our data indicate that these seals are host for parapoxvirus but are neither exposed to smooth Brucella spp. nor T. gondii . Evidence of PhHV-1 shedding was not detected. Article in Journal/Newspaper Antarc* Antarctic Antarctic Fur Seals Arctocephalus gazella Bouvetøya Elephant Seals Polar Research Southern Elephant Seals Southern Ocean University of Tromsø: Munin Open Research Archive Antarctic Austral Bouvetøya ENVELOPE(3.358,3.358,-54.422,-54.422) Southern Ocean Polar Research 39 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
Dyrehelse / Animal health Sel / Pinnipeds VDP::Mathematics and natural science: 400::Zoology and botany: 480 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480 |
spellingShingle |
Dyrehelse / Animal health Sel / Pinnipeds VDP::Mathematics and natural science: 400::Zoology and botany: 480 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480 Núñez-Egido, Sandra Lowther, Andrew Nymo, Ingebørg H. Klein, Jörn Breines, Eva Marie Tryland, Morten Pathogen surveillance in Southern Ocean pinnipeds |
topic_facet |
Dyrehelse / Animal health Sel / Pinnipeds VDP::Mathematics and natural science: 400::Zoology and botany: 480 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480 |
description |
Knowledge of the health status and potential effect of disease outbreaks among Southern Ocean fauna may be decisive for its conservation. We assessed the exposure and infection of Antarctic fur seals ( Arctocephalus gazella , AFS) and Southern elephant seals ( Mirounga leonine , SES) to parapoxvirus, Phocid alphaherpesvirus-1 (PhHV-1), smooth Brucella spp. and Toxoplasma gondii . AFS ( n = 65) serum and swab samples, and SES ( n = 13) serum samples from the sub--Antarctic island of Bouvetøya (54°25’S, 03°22’E) were collected during two austral summers (2014/15, 2017/18). Three polymerase chain reaction (PCR) tests amplifying the DNA polymerase, B2L and GIF parapoxvirus genomic regions were performed, investigating DNA from mucosal swab samples. The glycoprotein B gene was targeted to detect PhHV-1 viral DNA. Sera were assayed for T. gondii and smooth Brucella spp. antibodies with indirect enzyme-linked immunosorbent assays. Parapoxvirus PCR amplicons of the expected size were generated in two of the 29 AFS pups (nasal swabs, 2014/15), targeting the B2L ( n = 2) and DNA polymerase ( n = 1) genes, whereas the GIF PCR did not amplify target sequences. The PCR amplicons were sequenced and blasted in GenBank, revealing highest similarity with a seal parapoxvirus, confirming the presence of the virus in AFS for the first time. No PhHV-1 amplicons were generated, and antibodies against T. gondii or smooth Brucella spp. were not detected. Our data indicate that these seals are host for parapoxvirus but are neither exposed to smooth Brucella spp. nor T. gondii . Evidence of PhHV-1 shedding was not detected. |
format |
Article in Journal/Newspaper |
author |
Núñez-Egido, Sandra Lowther, Andrew Nymo, Ingebørg H. Klein, Jörn Breines, Eva Marie Tryland, Morten |
author_facet |
Núñez-Egido, Sandra Lowther, Andrew Nymo, Ingebørg H. Klein, Jörn Breines, Eva Marie Tryland, Morten |
author_sort |
Núñez-Egido, Sandra |
title |
Pathogen surveillance in Southern Ocean pinnipeds |
title_short |
Pathogen surveillance in Southern Ocean pinnipeds |
title_full |
Pathogen surveillance in Southern Ocean pinnipeds |
title_fullStr |
Pathogen surveillance in Southern Ocean pinnipeds |
title_full_unstemmed |
Pathogen surveillance in Southern Ocean pinnipeds |
title_sort |
pathogen surveillance in southern ocean pinnipeds |
publisher |
Norsk Polarinstitutt |
publishDate |
2020 |
url |
https://hdl.handle.net/10037/20722 https://doi.org/10.33265/polar.v39.3841 |
long_lat |
ENVELOPE(3.358,3.358,-54.422,-54.422) |
geographic |
Antarctic Austral Bouvetøya Southern Ocean |
geographic_facet |
Antarctic Austral Bouvetøya Southern Ocean |
genre |
Antarc* Antarctic Antarctic Fur Seals Arctocephalus gazella Bouvetøya Elephant Seals Polar Research Southern Elephant Seals Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctic Fur Seals Arctocephalus gazella Bouvetøya Elephant Seals Polar Research Southern Elephant Seals Southern Ocean |
op_relation |
Polar Research Núñez-Egido, Lowther, Nymo, Klein, Breines EM, Tryland M. Pathogen surveillance in Southern Ocean pinnipeds. Polar Research. 2020;39:1-11 FRIDAID 1865797 doi:10.33265/polar.v39.3841 0800-0395 1751-8369 https://hdl.handle.net/10037/20722 |
op_rights |
openAccess Copyright 2020 The Author(s) |
op_doi |
https://doi.org/10.33265/polar.v39.3841 |
container_title |
Polar Research |
container_volume |
39 |
_version_ |
1766257561071255552 |