Detection of specific Atlantic salmon antibodies against salmonid alphavirus using a bead-based immunoassay
Salmonid alphavirus (SAV) is the etiological cause of pancreas disease (PD) in Atlantic salmon ( Salmo salar ). Several vaccines against SAV are in use, but PD still cause significant mortality and concern in European aquaculture, raising the need for optimal tools to monitor SAV immunity. To monito...
Published in: | Fish & Shellfish Immunology |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Elsevier
2020
|
Subjects: | |
Online Access: | https://hdl.handle.net/10037/19701 https://doi.org/10.1016/j.fsi.2020.07.055 |
id |
ftunivtroemsoe:oai:munin.uit.no:10037/19701 |
---|---|
record_format |
openpolar |
spelling |
ftunivtroemsoe:oai:munin.uit.no:10037/19701 2023-05-15T15:31:46+02:00 Detection of specific Atlantic salmon antibodies against salmonid alphavirus using a bead-based immunoassay Teige, Lena Hammerlund Aksnes, Ida Røsæg, Magnus Vikan Jensen, Ingvill Jørgensen, Jorunn Sindre, Hilde Collins, Catherine Collet, Bertrand Rimstad, Espen Dahle, Maria Boysen, Preben 2020-07-30 https://hdl.handle.net/10037/19701 https://doi.org/10.1016/j.fsi.2020.07.055 eng eng Elsevier Fish and Shellfish Immunology info:eu-repo/grantAgreement/RCN/HAVBRUK2/237315/Norway/Fish Virus Vaccines/ViVaFish/ info:eu-repo/grantAgreement/RCN/HAVBRUK2?/280847/Norway/?/ViVaAct?/ Teige, Aksnes, Røsæg, Jensen, Jørgensen, Sindre, Collins, Collet, Rimstad, Dahle, Boysen. Detection of specific Atlantic salmon antibodies against salmonid alphavirus using a bead-based immunoassay. Fish and Shellfish Immunology. 2020 FRIDAID 1824581 doi:10.1016/j.fsi.2020.07.055 1050-4648 1095-9947 https://hdl.handle.net/10037/19701 openAccess Copyright 2020 The Author(s) VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 Journal article Tidsskriftartikkel Peer reviewed publishedVersion 2020 ftunivtroemsoe https://doi.org/10.1016/j.fsi.2020.07.055 2021-06-25T17:57:46Z Salmonid alphavirus (SAV) is the etiological cause of pancreas disease (PD) in Atlantic salmon ( Salmo salar ). Several vaccines against SAV are in use, but PD still cause significant mortality and concern in European aquaculture, raising the need for optimal tools to monitor SAV immunity. To monitor and control the distribution of PD in Norway, all salmonid farms are regularly screened for SAV by RT-qPCR. While the direct detection of SAV is helpful in the early stages of infection, serological methods could bring additional information on acquired SAV immunity in the later stages. Traditionally, SAV antibodies are monitored in neutralization assays, but they are time-consuming and cumbersome, thus alternative assays are warranted. Enzyme-linked immunosorbent assays (ELISAs) have not yet been successfully used for anti-SAV antibody detection in aquaculture. We aimed to develop a bead-based immunoassay for SAV-specific antibodies. By using detergent-treated SAV particles as antigens, we detected SAV-specific antibodies in plasma collected from both a SAV challenge trial and a field outbreak of PD. Increased levels of SAV-specific antibodies were seen after most fish had become negative for viral RNA. The bead-based assay is time saving compared to virus neutralization assays, and suitable for non-lethal testing due to low sample size requirements. We conclude that the bead-based immunoassay for SAV antibody detection is a promising diagnostic tool to complement SAV screening in aquaculture. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Tromsø: Munin Open Research Archive Norway Sav’ ENVELOPE(156.400,156.400,68.817,68.817) Fish & Shellfish Immunology 106 374 383 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 |
spellingShingle |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 Teige, Lena Hammerlund Aksnes, Ida Røsæg, Magnus Vikan Jensen, Ingvill Jørgensen, Jorunn Sindre, Hilde Collins, Catherine Collet, Bertrand Rimstad, Espen Dahle, Maria Boysen, Preben Detection of specific Atlantic salmon antibodies against salmonid alphavirus using a bead-based immunoassay |
topic_facet |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 |
description |
Salmonid alphavirus (SAV) is the etiological cause of pancreas disease (PD) in Atlantic salmon ( Salmo salar ). Several vaccines against SAV are in use, but PD still cause significant mortality and concern in European aquaculture, raising the need for optimal tools to monitor SAV immunity. To monitor and control the distribution of PD in Norway, all salmonid farms are regularly screened for SAV by RT-qPCR. While the direct detection of SAV is helpful in the early stages of infection, serological methods could bring additional information on acquired SAV immunity in the later stages. Traditionally, SAV antibodies are monitored in neutralization assays, but they are time-consuming and cumbersome, thus alternative assays are warranted. Enzyme-linked immunosorbent assays (ELISAs) have not yet been successfully used for anti-SAV antibody detection in aquaculture. We aimed to develop a bead-based immunoassay for SAV-specific antibodies. By using detergent-treated SAV particles as antigens, we detected SAV-specific antibodies in plasma collected from both a SAV challenge trial and a field outbreak of PD. Increased levels of SAV-specific antibodies were seen after most fish had become negative for viral RNA. The bead-based assay is time saving compared to virus neutralization assays, and suitable for non-lethal testing due to low sample size requirements. We conclude that the bead-based immunoassay for SAV antibody detection is a promising diagnostic tool to complement SAV screening in aquaculture. |
format |
Article in Journal/Newspaper |
author |
Teige, Lena Hammerlund Aksnes, Ida Røsæg, Magnus Vikan Jensen, Ingvill Jørgensen, Jorunn Sindre, Hilde Collins, Catherine Collet, Bertrand Rimstad, Espen Dahle, Maria Boysen, Preben |
author_facet |
Teige, Lena Hammerlund Aksnes, Ida Røsæg, Magnus Vikan Jensen, Ingvill Jørgensen, Jorunn Sindre, Hilde Collins, Catherine Collet, Bertrand Rimstad, Espen Dahle, Maria Boysen, Preben |
author_sort |
Teige, Lena Hammerlund |
title |
Detection of specific Atlantic salmon antibodies against salmonid alphavirus using a bead-based immunoassay |
title_short |
Detection of specific Atlantic salmon antibodies against salmonid alphavirus using a bead-based immunoassay |
title_full |
Detection of specific Atlantic salmon antibodies against salmonid alphavirus using a bead-based immunoassay |
title_fullStr |
Detection of specific Atlantic salmon antibodies against salmonid alphavirus using a bead-based immunoassay |
title_full_unstemmed |
Detection of specific Atlantic salmon antibodies against salmonid alphavirus using a bead-based immunoassay |
title_sort |
detection of specific atlantic salmon antibodies against salmonid alphavirus using a bead-based immunoassay |
publisher |
Elsevier |
publishDate |
2020 |
url |
https://hdl.handle.net/10037/19701 https://doi.org/10.1016/j.fsi.2020.07.055 |
long_lat |
ENVELOPE(156.400,156.400,68.817,68.817) |
geographic |
Norway Sav’ |
geographic_facet |
Norway Sav’ |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Fish and Shellfish Immunology info:eu-repo/grantAgreement/RCN/HAVBRUK2/237315/Norway/Fish Virus Vaccines/ViVaFish/ info:eu-repo/grantAgreement/RCN/HAVBRUK2?/280847/Norway/?/ViVaAct?/ Teige, Aksnes, Røsæg, Jensen, Jørgensen, Sindre, Collins, Collet, Rimstad, Dahle, Boysen. Detection of specific Atlantic salmon antibodies against salmonid alphavirus using a bead-based immunoassay. Fish and Shellfish Immunology. 2020 FRIDAID 1824581 doi:10.1016/j.fsi.2020.07.055 1050-4648 1095-9947 https://hdl.handle.net/10037/19701 |
op_rights |
openAccess Copyright 2020 The Author(s) |
op_doi |
https://doi.org/10.1016/j.fsi.2020.07.055 |
container_title |
Fish & Shellfish Immunology |
container_volume |
106 |
container_start_page |
374 |
op_container_end_page |
383 |
_version_ |
1766362284735594496 |