Oxidant-induced modifications in the mucosal transcriptome and circulating metabolome of Atlantic salmon
Here we report the molecular networks associated with the mucosal and systemic responses to peracetic acid (PAA), a candidate oxidative chemotherapeutic in Atlantic salmon ( Salmo salar ). Smolts were exposed to different therapeutic doses (0, 0.6 and 2.4 mg/L) of PAA for 5 min, followed by a re-exp...
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Online Access: | https://hdl.handle.net/10037/19433 https://doi.org/10.1016/j.aquatox.2020.105625 |
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ftunivtroemsoe:oai:munin.uit.no:10037/19433 2023-05-15T15:31:52+02:00 Oxidant-induced modifications in the mucosal transcriptome and circulating metabolome of Atlantic salmon Lazado, Carlo C. Pedersen, Lars-Flemming Kirste, Katrine H. Soleng, Malene Breiland, Mette Serine Wesmajervi Timmerhaus, Gerrit 2020-09-06 https://hdl.handle.net/10037/19433 https://doi.org/10.1016/j.aquatox.2020.105625 eng eng Elsevier Aquatic Toxicology Fiskeri- og havbruksnæringens forskningsfinansiering: 901472 Lazado CC, Pedersen L, Kirste K, Soleng M, Breiland Msw, Timmerhaus GF. Oxidant-induced modifications in the mucosal transcriptome and circulating metabolome of Atlantic salmon . Aquatic Toxicology. 2020;227 FRIDAID 1827066 doi:10.1016/j.aquatox.2020.105625 0166-445X 1879-1514 https://hdl.handle.net/10037/19433 openAccess Copyright 2020 The Author(s) VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 Journal article Tidsskriftartikkel Peer reviewed publishedVersion 2020 ftunivtroemsoe https://doi.org/10.1016/j.aquatox.2020.105625 2021-06-25T17:57:43Z Here we report the molecular networks associated with the mucosal and systemic responses to peracetic acid (PAA), a candidate oxidative chemotherapeutic in Atlantic salmon ( Salmo salar ). Smolts were exposed to different therapeutic doses (0, 0.6 and 2.4 mg/L) of PAA for 5 min, followed by a re-exposure to the same concentrations for 30 min 2 weeks later. PAA-exposed groups have higher external welfare score alterations, especially 2 weeks after the re-exposure. Cases of fin damage and scale loss were prevalent in the PAA-exposed groups. Transcriptomic profiling of mucosal tissues revealed that the skin had 12.5 % more differentially regulated genes (DEGs) than the gills following PAA exposure. The largest cluster of DEGs, both in the skin and gills, were involved in tissue extracellular matrix and metabolism. There were 22 DEGs common to both mucosal tissues, which were represented primarily by genes involved in the biophysical integrity of the mucosal barrier, including cadherin, collagen I α 2 chain, mucin-2 and spondin 1a . The absence of significant clustering in the plasma metabolomes amongst the three treatment groups indicates that PAA treatment did not induce any global metabolomic disturbances. Nonetheless, five metabolites with known functions during oxidative stress were remarkably affected by PAA treatments such as citrulline, histidine, tryptophan, methionine and trans-4-hydroxyproline. Collectively, these results indicate that salmon were able to mount mucosal and systemic adaptive responses to therapeutic doses of PAA and that the molecules identified are potential markers for assessing the health and welfare consequences of oxidant exposure. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Tromsø: Munin Open Research Archive Paa ENVELOPE(-53.483,-53.483,66.017,66.017) Aquatic Toxicology 227 105625 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 |
spellingShingle |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 Lazado, Carlo C. Pedersen, Lars-Flemming Kirste, Katrine H. Soleng, Malene Breiland, Mette Serine Wesmajervi Timmerhaus, Gerrit Oxidant-induced modifications in the mucosal transcriptome and circulating metabolome of Atlantic salmon |
topic_facet |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 |
description |
Here we report the molecular networks associated with the mucosal and systemic responses to peracetic acid (PAA), a candidate oxidative chemotherapeutic in Atlantic salmon ( Salmo salar ). Smolts were exposed to different therapeutic doses (0, 0.6 and 2.4 mg/L) of PAA for 5 min, followed by a re-exposure to the same concentrations for 30 min 2 weeks later. PAA-exposed groups have higher external welfare score alterations, especially 2 weeks after the re-exposure. Cases of fin damage and scale loss were prevalent in the PAA-exposed groups. Transcriptomic profiling of mucosal tissues revealed that the skin had 12.5 % more differentially regulated genes (DEGs) than the gills following PAA exposure. The largest cluster of DEGs, both in the skin and gills, were involved in tissue extracellular matrix and metabolism. There were 22 DEGs common to both mucosal tissues, which were represented primarily by genes involved in the biophysical integrity of the mucosal barrier, including cadherin, collagen I α 2 chain, mucin-2 and spondin 1a . The absence of significant clustering in the plasma metabolomes amongst the three treatment groups indicates that PAA treatment did not induce any global metabolomic disturbances. Nonetheless, five metabolites with known functions during oxidative stress were remarkably affected by PAA treatments such as citrulline, histidine, tryptophan, methionine and trans-4-hydroxyproline. Collectively, these results indicate that salmon were able to mount mucosal and systemic adaptive responses to therapeutic doses of PAA and that the molecules identified are potential markers for assessing the health and welfare consequences of oxidant exposure. |
format |
Article in Journal/Newspaper |
author |
Lazado, Carlo C. Pedersen, Lars-Flemming Kirste, Katrine H. Soleng, Malene Breiland, Mette Serine Wesmajervi Timmerhaus, Gerrit |
author_facet |
Lazado, Carlo C. Pedersen, Lars-Flemming Kirste, Katrine H. Soleng, Malene Breiland, Mette Serine Wesmajervi Timmerhaus, Gerrit |
author_sort |
Lazado, Carlo C. |
title |
Oxidant-induced modifications in the mucosal transcriptome and circulating metabolome of Atlantic salmon |
title_short |
Oxidant-induced modifications in the mucosal transcriptome and circulating metabolome of Atlantic salmon |
title_full |
Oxidant-induced modifications in the mucosal transcriptome and circulating metabolome of Atlantic salmon |
title_fullStr |
Oxidant-induced modifications in the mucosal transcriptome and circulating metabolome of Atlantic salmon |
title_full_unstemmed |
Oxidant-induced modifications in the mucosal transcriptome and circulating metabolome of Atlantic salmon |
title_sort |
oxidant-induced modifications in the mucosal transcriptome and circulating metabolome of atlantic salmon |
publisher |
Elsevier |
publishDate |
2020 |
url |
https://hdl.handle.net/10037/19433 https://doi.org/10.1016/j.aquatox.2020.105625 |
long_lat |
ENVELOPE(-53.483,-53.483,66.017,66.017) |
geographic |
Paa |
geographic_facet |
Paa |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Aquatic Toxicology Fiskeri- og havbruksnæringens forskningsfinansiering: 901472 Lazado CC, Pedersen L, Kirste K, Soleng M, Breiland Msw, Timmerhaus GF. Oxidant-induced modifications in the mucosal transcriptome and circulating metabolome of Atlantic salmon . Aquatic Toxicology. 2020;227 FRIDAID 1827066 doi:10.1016/j.aquatox.2020.105625 0166-445X 1879-1514 https://hdl.handle.net/10037/19433 |
op_rights |
openAccess Copyright 2020 The Author(s) |
op_doi |
https://doi.org/10.1016/j.aquatox.2020.105625 |
container_title |
Aquatic Toxicology |
container_volume |
227 |
container_start_page |
105625 |
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1766362376630697984 |