Dissemination of Piscine orthoreovirus-1 (PRV-1) in Atlantic Salmon (Salmo salar) during the Early and Regenerating Phases of Infection
Piscine orthoreovirus-1 (PRV-1) can cause heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon ( Salmo salar ), but the line of events from infection, pathologic change, and regeneration has not been thoroughly described. In this study, the cellular localization and variation of P...
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ftunivtroemsoe:oai:munin.uit.no:10037/19145 2023-05-15T15:30:36+02:00 Dissemination of Piscine orthoreovirus-1 (PRV-1) in Atlantic Salmon (Salmo salar) during the Early and Regenerating Phases of Infection Dhamotharan, Kannimuthu Bjørgen, Håvard Malik, Muhammad Salman Nyman, Ingvild Berg Markussen, Turhan Ali Dadasbilge Dahle, Maria Koppang, Erling Olaf Wessel, Øystein Rimstad, Espen 2020-02-20 https://hdl.handle.net/10037/19145 https://doi.org/10.3390/pathogens9020143 eng eng MDPI Pathogens Norges forskningsråd: 280847 info:eu-repo/grantAgreement/RCN/HAVBRUK2/280847/Norway/Comparing the protection of attenuated and inactivated virus vaccines against pancreas disease and heart and skeletal muscle inflammation// Kannimuthu, Bjørgen, Malik, Nyman, Markussen, Dahle, Koppang, Wessel, Rimstad. Dissemination of Piscine orthoreovirus-1 (PRV-1) in Atlantic Salmon (Salmo salar) during the Early and Regenerating Phases of Infection. Pathogens. 2020 FRIDAID 1824582 doi:10.3390/pathogens9020143 2076-0817 https://hdl.handle.net/10037/19145 openAccess Copyright 2020 The Author(s) VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 Journal article Tidsskriftartikkel Peer reviewed publishedVersion 2020 ftunivtroemsoe https://doi.org/10.3390/pathogens9020143 2021-06-25T17:57:37Z Piscine orthoreovirus-1 (PRV-1) can cause heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon ( Salmo salar ), but the line of events from infection, pathologic change, and regeneration has not been thoroughly described. In this study, the cellular localization and variation of PRV-1 RNA and protein levels were analyzed at different times post-exposure in experimentally infected Atlantic salmon. Immunohistochemistry, flow cytometry, and Western blot were used for assessment of the presence of the PRV-1 σ1 protein, while RT-qPCR and in situ hybridization were performed for viral RNA. Histopathologic evaluation demonstrated that PRV-1 infection induced heart lesions typical of HSMI, such as severe epicarditis and myocarditis with degeneration of cardiomyocytes, necrosis, and diffuse cellular infiltration. PRV-1 infection of erythrocytes and the peak viral plasma level preceded virus presence in cardiomyocytes and hepatocytes. Arginase-2-positive, macrophage-like cells observed in the heart indicated possible polarization to M2 macrophages and the onset of regenerative processes, which may contribute to the recovery from HSMI. The virus was cleared from regenerating heart tissue and from hepatocytes, but persisted in erythrocytes. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Tromsø: Munin Open Research Archive Pathogens 9 2 143 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 |
spellingShingle |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 Dhamotharan, Kannimuthu Bjørgen, Håvard Malik, Muhammad Salman Nyman, Ingvild Berg Markussen, Turhan Ali Dadasbilge Dahle, Maria Koppang, Erling Olaf Wessel, Øystein Rimstad, Espen Dissemination of Piscine orthoreovirus-1 (PRV-1) in Atlantic Salmon (Salmo salar) during the Early and Regenerating Phases of Infection |
topic_facet |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 |
description |
Piscine orthoreovirus-1 (PRV-1) can cause heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon ( Salmo salar ), but the line of events from infection, pathologic change, and regeneration has not been thoroughly described. In this study, the cellular localization and variation of PRV-1 RNA and protein levels were analyzed at different times post-exposure in experimentally infected Atlantic salmon. Immunohistochemistry, flow cytometry, and Western blot were used for assessment of the presence of the PRV-1 σ1 protein, while RT-qPCR and in situ hybridization were performed for viral RNA. Histopathologic evaluation demonstrated that PRV-1 infection induced heart lesions typical of HSMI, such as severe epicarditis and myocarditis with degeneration of cardiomyocytes, necrosis, and diffuse cellular infiltration. PRV-1 infection of erythrocytes and the peak viral plasma level preceded virus presence in cardiomyocytes and hepatocytes. Arginase-2-positive, macrophage-like cells observed in the heart indicated possible polarization to M2 macrophages and the onset of regenerative processes, which may contribute to the recovery from HSMI. The virus was cleared from regenerating heart tissue and from hepatocytes, but persisted in erythrocytes. |
format |
Article in Journal/Newspaper |
author |
Dhamotharan, Kannimuthu Bjørgen, Håvard Malik, Muhammad Salman Nyman, Ingvild Berg Markussen, Turhan Ali Dadasbilge Dahle, Maria Koppang, Erling Olaf Wessel, Øystein Rimstad, Espen |
author_facet |
Dhamotharan, Kannimuthu Bjørgen, Håvard Malik, Muhammad Salman Nyman, Ingvild Berg Markussen, Turhan Ali Dadasbilge Dahle, Maria Koppang, Erling Olaf Wessel, Øystein Rimstad, Espen |
author_sort |
Dhamotharan, Kannimuthu |
title |
Dissemination of Piscine orthoreovirus-1 (PRV-1) in Atlantic Salmon (Salmo salar) during the Early and Regenerating Phases of Infection |
title_short |
Dissemination of Piscine orthoreovirus-1 (PRV-1) in Atlantic Salmon (Salmo salar) during the Early and Regenerating Phases of Infection |
title_full |
Dissemination of Piscine orthoreovirus-1 (PRV-1) in Atlantic Salmon (Salmo salar) during the Early and Regenerating Phases of Infection |
title_fullStr |
Dissemination of Piscine orthoreovirus-1 (PRV-1) in Atlantic Salmon (Salmo salar) during the Early and Regenerating Phases of Infection |
title_full_unstemmed |
Dissemination of Piscine orthoreovirus-1 (PRV-1) in Atlantic Salmon (Salmo salar) during the Early and Regenerating Phases of Infection |
title_sort |
dissemination of piscine orthoreovirus-1 (prv-1) in atlantic salmon (salmo salar) during the early and regenerating phases of infection |
publisher |
MDPI |
publishDate |
2020 |
url |
https://hdl.handle.net/10037/19145 https://doi.org/10.3390/pathogens9020143 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Pathogens Norges forskningsråd: 280847 info:eu-repo/grantAgreement/RCN/HAVBRUK2/280847/Norway/Comparing the protection of attenuated and inactivated virus vaccines against pancreas disease and heart and skeletal muscle inflammation// Kannimuthu, Bjørgen, Malik, Nyman, Markussen, Dahle, Koppang, Wessel, Rimstad. Dissemination of Piscine orthoreovirus-1 (PRV-1) in Atlantic Salmon (Salmo salar) during the Early and Regenerating Phases of Infection. Pathogens. 2020 FRIDAID 1824582 doi:10.3390/pathogens9020143 2076-0817 https://hdl.handle.net/10037/19145 |
op_rights |
openAccess Copyright 2020 The Author(s) |
op_doi |
https://doi.org/10.3390/pathogens9020143 |
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Pathogens |
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9 |
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2 |
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143 |
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1766361053298425856 |