A Widely Distributed Thraustochytrid Parasite of Diatoms Isolated from the Arctic Represents a gen. and sp. nov.
A unicellular, heterotrophic, eukaryotic parasite was isolated from nearshore Arctic marine sediment in association with the diatom Pleurosigma sp. The parasite possessed ectoplasmic threads that could penetrate diatom frustules. Healthy and reproducing Pleurosigma cultures would begin to collapse w...
Published in: | Journal of Eukaryotic Microbiology |
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Online Access: | https://hdl.handle.net/10037/19021 https://doi.org/10.1111/jeu.12796 |
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ftunivtroemsoe:oai:munin.uit.no:10037/19021 2023-05-15T14:25:33+02:00 A Widely Distributed Thraustochytrid Parasite of Diatoms Isolated from the Arctic Represents a gen. and sp. nov. Hassett, Brandon 2020-04-06 https://hdl.handle.net/10037/19021 https://doi.org/10.1111/jeu.12796 eng eng Wiley Journal of Eukaryotic Microbiology Norges forskningsråd: 01vm/h15 Hassett BT. A Widely Distributed Thraustochytrid Parasite of Diatoms Isolated from the Arctic Represents a gen. and sp. nov. Journal of Eukaryotic Microbiology. 2020;67:480-490 FRIDAID 1812062 doi:10.1111/jeu.12796 1066-5234 1550-7408 https://hdl.handle.net/10037/19021 openAccess Copyright 2020 The Author(s) VDP::Mathematics and natural science: 400::Zoology and botany: 480 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480 Journal article Tidsskriftartikkel Peer reviewed publishedVersion 2020 ftunivtroemsoe https://doi.org/10.1111/jeu.12796 2021-06-25T17:57:36Z A unicellular, heterotrophic, eukaryotic parasite was isolated from nearshore Arctic marine sediment in association with the diatom Pleurosigma sp. The parasite possessed ectoplasmic threads that could penetrate diatom frustules. Healthy and reproducing Pleurosigma cultures would begin to collapse within a week following the introduction of this parasite. The parasite (2–10 μm diameter) could reproduce epibiotically with biflagellate zoospores, as well as binary division inside and outside the diatom host. While the parasite grew, diatom intracellular content disappeared. Evaluation of electron micrographs from co‐cultures revealed the presence of hollow tubular processes and amorphic cells that could transcend the diatom frustule, generally at the girdle band, as well as typical thraustochytrid ultrastructure, such as the presence of bothrosomes. After nucleotide extraction, amplification, and cloning, database queries of DNA revealed closest molecular affinity to environmental thraustochytrid clone sequences. Testing of phylogenetic hypotheses consistently grouped this unknown parasite within the Thraustochytriidae on a distinct branch within the environmental sequence clade Lab19. Reclassification of Arctic high‐throughput sequencing data, with appended reference datasets that included this diatom parasite, indicated that the majority of thraustochytrid sequences, previously binned as unclassifiable stramenopiles, are allied to this new isolate. Based on the combined information acquired from electron microscopy, life history, and phylogenetic testing, this unknown isolate is described as a novel species and genus. Article in Journal/Newspaper Arctic Arctic University of Tromsø: Munin Open Research Archive Arctic Journal of Eukaryotic Microbiology 67 4 480 490 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Mathematics and natural science: 400::Zoology and botany: 480 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480 |
spellingShingle |
VDP::Mathematics and natural science: 400::Zoology and botany: 480 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480 Hassett, Brandon A Widely Distributed Thraustochytrid Parasite of Diatoms Isolated from the Arctic Represents a gen. and sp. nov. |
topic_facet |
VDP::Mathematics and natural science: 400::Zoology and botany: 480 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480 |
description |
A unicellular, heterotrophic, eukaryotic parasite was isolated from nearshore Arctic marine sediment in association with the diatom Pleurosigma sp. The parasite possessed ectoplasmic threads that could penetrate diatom frustules. Healthy and reproducing Pleurosigma cultures would begin to collapse within a week following the introduction of this parasite. The parasite (2–10 μm diameter) could reproduce epibiotically with biflagellate zoospores, as well as binary division inside and outside the diatom host. While the parasite grew, diatom intracellular content disappeared. Evaluation of electron micrographs from co‐cultures revealed the presence of hollow tubular processes and amorphic cells that could transcend the diatom frustule, generally at the girdle band, as well as typical thraustochytrid ultrastructure, such as the presence of bothrosomes. After nucleotide extraction, amplification, and cloning, database queries of DNA revealed closest molecular affinity to environmental thraustochytrid clone sequences. Testing of phylogenetic hypotheses consistently grouped this unknown parasite within the Thraustochytriidae on a distinct branch within the environmental sequence clade Lab19. Reclassification of Arctic high‐throughput sequencing data, with appended reference datasets that included this diatom parasite, indicated that the majority of thraustochytrid sequences, previously binned as unclassifiable stramenopiles, are allied to this new isolate. Based on the combined information acquired from electron microscopy, life history, and phylogenetic testing, this unknown isolate is described as a novel species and genus. |
format |
Article in Journal/Newspaper |
author |
Hassett, Brandon |
author_facet |
Hassett, Brandon |
author_sort |
Hassett, Brandon |
title |
A Widely Distributed Thraustochytrid Parasite of Diatoms Isolated from the Arctic Represents a gen. and sp. nov. |
title_short |
A Widely Distributed Thraustochytrid Parasite of Diatoms Isolated from the Arctic Represents a gen. and sp. nov. |
title_full |
A Widely Distributed Thraustochytrid Parasite of Diatoms Isolated from the Arctic Represents a gen. and sp. nov. |
title_fullStr |
A Widely Distributed Thraustochytrid Parasite of Diatoms Isolated from the Arctic Represents a gen. and sp. nov. |
title_full_unstemmed |
A Widely Distributed Thraustochytrid Parasite of Diatoms Isolated from the Arctic Represents a gen. and sp. nov. |
title_sort |
widely distributed thraustochytrid parasite of diatoms isolated from the arctic represents a gen. and sp. nov. |
publisher |
Wiley |
publishDate |
2020 |
url |
https://hdl.handle.net/10037/19021 https://doi.org/10.1111/jeu.12796 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Arctic |
genre_facet |
Arctic Arctic |
op_relation |
Journal of Eukaryotic Microbiology Norges forskningsråd: 01vm/h15 Hassett BT. A Widely Distributed Thraustochytrid Parasite of Diatoms Isolated from the Arctic Represents a gen. and sp. nov. Journal of Eukaryotic Microbiology. 2020;67:480-490 FRIDAID 1812062 doi:10.1111/jeu.12796 1066-5234 1550-7408 https://hdl.handle.net/10037/19021 |
op_rights |
openAccess Copyright 2020 The Author(s) |
op_doi |
https://doi.org/10.1111/jeu.12796 |
container_title |
Journal of Eukaryotic Microbiology |
container_volume |
67 |
container_issue |
4 |
container_start_page |
480 |
op_container_end_page |
490 |
_version_ |
1766297984872480768 |