Promiscuous T cell epitopes boosts specific IgM immune response against a P0 peptide antigen from sea lice in different teleost species
The development of vaccines employing conserved protein antigens, for instance ribosomal protein P0, has as disadvantage the high degree of identity between pathogen and host proteins due to possible induction of tolerance or auto antibodies in the host organism. To overcome this drawback, peptide-b...
Published in: | Fish & Shellfish Immunology |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Elsevier
2019
|
Subjects: | |
Online Access: | https://hdl.handle.net/10037/17281 https://doi.org/10.1016/j.fsi.2019.06.018 |
id |
ftunivtroemsoe:oai:munin.uit.no:10037/17281 |
---|---|
record_format |
openpolar |
spelling |
ftunivtroemsoe:oai:munin.uit.no:10037/17281 2023-05-15T15:32:56+02:00 Promiscuous T cell epitopes boosts specific IgM immune response against a P0 peptide antigen from sea lice in different teleost species Leal, Yeny Velazquez, Janet Hernandez, Liz Swain, Jaya Kumari Rodríguez, Alianet Rodríguez Martínez, Rebeca García, Claudia Ramos, Yassel Estrada, Mario Pablo Carpio, Yamila 2019-06-11 https://hdl.handle.net/10037/17281 https://doi.org/10.1016/j.fsi.2019.06.018 eng eng Elsevier Fish and Shellfish Immunology Fiskeri- og havbruksnæringens forskningsfinansiering: 901461 Nofima AS: 12211 Leal, Velazquez, Hernandez, Swain K, Rodríguez, Martínez, García, Ramos, Estrada, Carpio. Promiscuous T cell epitopes boosts specific IgM immune response against a P0 peptide antigen from sea lice in different teleost species. Fish and Shellfish Immunology. 2019;92:322-330 FRIDAID 1712057 doi:10.1016/j.fsi.2019.06.018 1050-4648 1095-9947 https://hdl.handle.net/10037/17281 openAccess © 2019 Elsevier Ltd. All rights reserved. VDP::Medical disciplines: 700::Basic medical dental and veterinary science disciplines: 710 VDP::Medisinske Fag: 700::Basale medisinske odontologiske og veterinærmedisinske fag: 710 Journal article Tidsskriftartikkel Preprint Manuskript submittedVersion 2019 ftunivtroemsoe https://doi.org/10.1016/j.fsi.2019.06.018 2021-06-25T17:57:11Z The development of vaccines employing conserved protein antigens, for instance ribosomal protein P0, has as disadvantage the high degree of identity between pathogen and host proteins due to possible induction of tolerance or auto antibodies in the host organism. To overcome this drawback, peptide-based vaccines have been designed with a proved high efficacy. The use of defined peptides as antigens has the problem that they are generally poor immunogenic unless coupled to a carrier protein. Several studies have established the potential for promiscuous T cell epitopes incorporated into chimeric peptides to enhance the immunogenicity in mammals. On the contrary, studies about the role of these epitopes on teleost immune system are scarce. Therefore, the main objective of our present study was to evaluate the potential of promiscuous T cell epitopes to boost specific IgM immune response in teleost fish against a peptide antigen. With this aim, we used a peptide of 35 amino acids from the ribosomal P0 protein of Lepeophtheirus salmonis , an important parasite in salmon aquaculture. We fused this peptide to the C-terminal of T cell epitopes from tetanus toxin and measles virus and produced the chimeric protein in Escherichia coli . Following vaccination, IgM antibody production was monitored in different immunization schemes in Tilapia, African catfish and Atlantic salmon. The results demonstrated for first time that the addition of T cell epitopes at the N-terminal of a target peptide increased IgM specific response in different teleost species, revealing the potential of this approach to develop peptide-based vaccines for aquaculture. The results are also of great importance in the context of vaccine development against sea lice using ribosomal protein P0 as antigen taking into account the key role of P0 in protein synthesis and other essential physiological processes. Article in Journal/Newspaper Atlantic salmon University of Tromsø: Munin Open Research Archive Fish & Shellfish Immunology 92 322 330 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Medical disciplines: 700::Basic medical dental and veterinary science disciplines: 710 VDP::Medisinske Fag: 700::Basale medisinske odontologiske og veterinærmedisinske fag: 710 |
spellingShingle |
VDP::Medical disciplines: 700::Basic medical dental and veterinary science disciplines: 710 VDP::Medisinske Fag: 700::Basale medisinske odontologiske og veterinærmedisinske fag: 710 Leal, Yeny Velazquez, Janet Hernandez, Liz Swain, Jaya Kumari Rodríguez, Alianet Rodríguez Martínez, Rebeca García, Claudia Ramos, Yassel Estrada, Mario Pablo Carpio, Yamila Promiscuous T cell epitopes boosts specific IgM immune response against a P0 peptide antigen from sea lice in different teleost species |
topic_facet |
VDP::Medical disciplines: 700::Basic medical dental and veterinary science disciplines: 710 VDP::Medisinske Fag: 700::Basale medisinske odontologiske og veterinærmedisinske fag: 710 |
description |
The development of vaccines employing conserved protein antigens, for instance ribosomal protein P0, has as disadvantage the high degree of identity between pathogen and host proteins due to possible induction of tolerance or auto antibodies in the host organism. To overcome this drawback, peptide-based vaccines have been designed with a proved high efficacy. The use of defined peptides as antigens has the problem that they are generally poor immunogenic unless coupled to a carrier protein. Several studies have established the potential for promiscuous T cell epitopes incorporated into chimeric peptides to enhance the immunogenicity in mammals. On the contrary, studies about the role of these epitopes on teleost immune system are scarce. Therefore, the main objective of our present study was to evaluate the potential of promiscuous T cell epitopes to boost specific IgM immune response in teleost fish against a peptide antigen. With this aim, we used a peptide of 35 amino acids from the ribosomal P0 protein of Lepeophtheirus salmonis , an important parasite in salmon aquaculture. We fused this peptide to the C-terminal of T cell epitopes from tetanus toxin and measles virus and produced the chimeric protein in Escherichia coli . Following vaccination, IgM antibody production was monitored in different immunization schemes in Tilapia, African catfish and Atlantic salmon. The results demonstrated for first time that the addition of T cell epitopes at the N-terminal of a target peptide increased IgM specific response in different teleost species, revealing the potential of this approach to develop peptide-based vaccines for aquaculture. The results are also of great importance in the context of vaccine development against sea lice using ribosomal protein P0 as antigen taking into account the key role of P0 in protein synthesis and other essential physiological processes. |
format |
Article in Journal/Newspaper |
author |
Leal, Yeny Velazquez, Janet Hernandez, Liz Swain, Jaya Kumari Rodríguez, Alianet Rodríguez Martínez, Rebeca García, Claudia Ramos, Yassel Estrada, Mario Pablo Carpio, Yamila |
author_facet |
Leal, Yeny Velazquez, Janet Hernandez, Liz Swain, Jaya Kumari Rodríguez, Alianet Rodríguez Martínez, Rebeca García, Claudia Ramos, Yassel Estrada, Mario Pablo Carpio, Yamila |
author_sort |
Leal, Yeny |
title |
Promiscuous T cell epitopes boosts specific IgM immune response against a P0 peptide antigen from sea lice in different teleost species |
title_short |
Promiscuous T cell epitopes boosts specific IgM immune response against a P0 peptide antigen from sea lice in different teleost species |
title_full |
Promiscuous T cell epitopes boosts specific IgM immune response against a P0 peptide antigen from sea lice in different teleost species |
title_fullStr |
Promiscuous T cell epitopes boosts specific IgM immune response against a P0 peptide antigen from sea lice in different teleost species |
title_full_unstemmed |
Promiscuous T cell epitopes boosts specific IgM immune response against a P0 peptide antigen from sea lice in different teleost species |
title_sort |
promiscuous t cell epitopes boosts specific igm immune response against a p0 peptide antigen from sea lice in different teleost species |
publisher |
Elsevier |
publishDate |
2019 |
url |
https://hdl.handle.net/10037/17281 https://doi.org/10.1016/j.fsi.2019.06.018 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
Fish and Shellfish Immunology Fiskeri- og havbruksnæringens forskningsfinansiering: 901461 Nofima AS: 12211 Leal, Velazquez, Hernandez, Swain K, Rodríguez, Martínez, García, Ramos, Estrada, Carpio. Promiscuous T cell epitopes boosts specific IgM immune response against a P0 peptide antigen from sea lice in different teleost species. Fish and Shellfish Immunology. 2019;92:322-330 FRIDAID 1712057 doi:10.1016/j.fsi.2019.06.018 1050-4648 1095-9947 https://hdl.handle.net/10037/17281 |
op_rights |
openAccess © 2019 Elsevier Ltd. All rights reserved. |
op_doi |
https://doi.org/10.1016/j.fsi.2019.06.018 |
container_title |
Fish & Shellfish Immunology |
container_volume |
92 |
container_start_page |
322 |
op_container_end_page |
330 |
_version_ |
1766363412435042304 |