Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp.
Two bacterial isolates from the Barents Sea, both belonging to the genus Algibacter , were found to yield extracts with anti-bacterial bioactivity. Mass spectrometry guided dereplication and purification of the active extracts lead to the isolation of the same active principle in both extracts. The...
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Online Access: | https://hdl.handle.net/10037/16686 https://doi.org/10.3390/molecules24213991 |
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ftunivtroemsoe:oai:munin.uit.no:10037/16686 2023-05-15T15:38:54+02:00 Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. Schneider, Yannik Karl-Heinz Østnes Hansen, Kine Isaksson, Johan Ullsten-Wahlund, Sara Maria Hansen, Espen Andersen, Jeanette Hammer 2019-11-05 https://hdl.handle.net/10037/16686 https://doi.org/10.3390/molecules24213991 eng eng MDPI Schneider, Y.K.-H. (2020). Bioactive secondary metabolites from bacteria. Natural products from marine and terrestrial bacteria, dereplication, isolation and investigation of bacterial secondary metabolites. (Doctoral thesis). https://hdl.handle.net/10037/19264 Molecules info:eu-repo/grantAgreement/EC/H2020/721421/EU/Improving the flow in the pipeline of the next generation of marine biodiscovery scientists/MarPipe/ Schneider, Y.K.H., Hansen, K.Ø., Isaksson, J., Ullsten, S., Hansen, E.H. & Andersen, J.H. (2019). Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. Molecules, 24 (21), 3991. https://doi.org/10.3390/molecules24213991 FRIDAID 1744987 doi:10.3390/molecules24213991 1420-3049 https://hdl.handle.net/10037/16686 openAccess VDP::Matematikk og naturvitenskap: 400::Basale biofag: 470::Biokjemi: 476 VDP::Mathematics and natural scienses: 400::Basic biosciences: 470::Biochemistry: 476 Journal article Tidsskriftartikkel Peer reviewed 2019 ftunivtroemsoe https://doi.org/10.3390/molecules24213991 2021-06-25T17:56:56Z Two bacterial isolates from the Barents Sea, both belonging to the genus Algibacter , were found to yield extracts with anti-bacterial bioactivity. Mass spectrometry guided dereplication and purification of the active extracts lead to the isolation of the same active principle in both extracts. The structure of the bioactive compound was identified via mass spectrometry and nuclear resonance spectroscopy and it turned out to be the known lipopeptide Lipid 430. We discovered and determined its previously unknown anti-bacterial activity against Streptococcus agalactiae and revealed a cytotoxic effect against the A2058 human melanoma cell line at significantly lower concentrations compared to its anti-bacterial concentration. Flow cytometry and microscopy investigations of the cytotoxicity against the melanoma cell line indicated that Lipid 430 did not cause immediate cell lysis. The experiments with melanoma cells suggest that the compound functions trough more complex pathways than acting as a simple detergent. Article in Journal/Newspaper Barents Sea University of Tromsø: Munin Open Research Archive Barents Sea Molecules 24 21 3991 |
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Open Polar |
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University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Matematikk og naturvitenskap: 400::Basale biofag: 470::Biokjemi: 476 VDP::Mathematics and natural scienses: 400::Basic biosciences: 470::Biochemistry: 476 |
spellingShingle |
VDP::Matematikk og naturvitenskap: 400::Basale biofag: 470::Biokjemi: 476 VDP::Mathematics and natural scienses: 400::Basic biosciences: 470::Biochemistry: 476 Schneider, Yannik Karl-Heinz Østnes Hansen, Kine Isaksson, Johan Ullsten-Wahlund, Sara Maria Hansen, Espen Andersen, Jeanette Hammer Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. |
topic_facet |
VDP::Matematikk og naturvitenskap: 400::Basale biofag: 470::Biokjemi: 476 VDP::Mathematics and natural scienses: 400::Basic biosciences: 470::Biochemistry: 476 |
description |
Two bacterial isolates from the Barents Sea, both belonging to the genus Algibacter , were found to yield extracts with anti-bacterial bioactivity. Mass spectrometry guided dereplication and purification of the active extracts lead to the isolation of the same active principle in both extracts. The structure of the bioactive compound was identified via mass spectrometry and nuclear resonance spectroscopy and it turned out to be the known lipopeptide Lipid 430. We discovered and determined its previously unknown anti-bacterial activity against Streptococcus agalactiae and revealed a cytotoxic effect against the A2058 human melanoma cell line at significantly lower concentrations compared to its anti-bacterial concentration. Flow cytometry and microscopy investigations of the cytotoxicity against the melanoma cell line indicated that Lipid 430 did not cause immediate cell lysis. The experiments with melanoma cells suggest that the compound functions trough more complex pathways than acting as a simple detergent. |
format |
Article in Journal/Newspaper |
author |
Schneider, Yannik Karl-Heinz Østnes Hansen, Kine Isaksson, Johan Ullsten-Wahlund, Sara Maria Hansen, Espen Andersen, Jeanette Hammer |
author_facet |
Schneider, Yannik Karl-Heinz Østnes Hansen, Kine Isaksson, Johan Ullsten-Wahlund, Sara Maria Hansen, Espen Andersen, Jeanette Hammer |
author_sort |
Schneider, Yannik Karl-Heinz |
title |
Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. |
title_short |
Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. |
title_full |
Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. |
title_fullStr |
Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. |
title_full_unstemmed |
Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. |
title_sort |
anti-bacterial effect and cytotoxicity assessment of lipid 430 isolated from algibacter sp. |
publisher |
MDPI |
publishDate |
2019 |
url |
https://hdl.handle.net/10037/16686 https://doi.org/10.3390/molecules24213991 |
geographic |
Barents Sea |
geographic_facet |
Barents Sea |
genre |
Barents Sea |
genre_facet |
Barents Sea |
op_relation |
Schneider, Y.K.-H. (2020). Bioactive secondary metabolites from bacteria. Natural products from marine and terrestrial bacteria, dereplication, isolation and investigation of bacterial secondary metabolites. (Doctoral thesis). https://hdl.handle.net/10037/19264 Molecules info:eu-repo/grantAgreement/EC/H2020/721421/EU/Improving the flow in the pipeline of the next generation of marine biodiscovery scientists/MarPipe/ Schneider, Y.K.H., Hansen, K.Ø., Isaksson, J., Ullsten, S., Hansen, E.H. & Andersen, J.H. (2019). Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. Molecules, 24 (21), 3991. https://doi.org/10.3390/molecules24213991 FRIDAID 1744987 doi:10.3390/molecules24213991 1420-3049 https://hdl.handle.net/10037/16686 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.3390/molecules24213991 |
container_title |
Molecules |
container_volume |
24 |
container_issue |
21 |
container_start_page |
3991 |
_version_ |
1766370318512816128 |