Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp.

Two bacterial isolates from the Barents Sea, both belonging to the genus Algibacter , were found to yield extracts with anti-bacterial bioactivity. Mass spectrometry guided dereplication and purification of the active extracts lead to the isolation of the same active principle in both extracts. The...

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Published in:Molecules
Main Authors: Schneider, Yannik Karl-Heinz, Østnes Hansen, Kine, Isaksson, Johan, Ullsten-Wahlund, Sara Maria, Hansen, Espen, Andersen, Jeanette Hammer
Format: Article in Journal/Newspaper
Language:English
Published: MDPI 2019
Subjects:
Online Access:https://hdl.handle.net/10037/16686
https://doi.org/10.3390/molecules24213991
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spelling ftunivtroemsoe:oai:munin.uit.no:10037/16686 2023-05-15T15:38:54+02:00 Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. Schneider, Yannik Karl-Heinz Østnes Hansen, Kine Isaksson, Johan Ullsten-Wahlund, Sara Maria Hansen, Espen Andersen, Jeanette Hammer 2019-11-05 https://hdl.handle.net/10037/16686 https://doi.org/10.3390/molecules24213991 eng eng MDPI Schneider, Y.K.-H. (2020). Bioactive secondary metabolites from bacteria. Natural products from marine and terrestrial bacteria, dereplication, isolation and investigation of bacterial secondary metabolites. (Doctoral thesis). https://hdl.handle.net/10037/19264 Molecules info:eu-repo/grantAgreement/EC/H2020/721421/EU/Improving the flow in the pipeline of the next generation of marine biodiscovery scientists/MarPipe/ Schneider, Y.K.H., Hansen, K.Ø., Isaksson, J., Ullsten, S., Hansen, E.H. & Andersen, J.H. (2019). Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. Molecules, 24 (21), 3991. https://doi.org/10.3390/molecules24213991 FRIDAID 1744987 doi:10.3390/molecules24213991 1420-3049 https://hdl.handle.net/10037/16686 openAccess VDP::Matematikk og naturvitenskap: 400::Basale biofag: 470::Biokjemi: 476 VDP::Mathematics and natural scienses: 400::Basic biosciences: 470::Biochemistry: 476 Journal article Tidsskriftartikkel Peer reviewed 2019 ftunivtroemsoe https://doi.org/10.3390/molecules24213991 2021-06-25T17:56:56Z Two bacterial isolates from the Barents Sea, both belonging to the genus Algibacter , were found to yield extracts with anti-bacterial bioactivity. Mass spectrometry guided dereplication and purification of the active extracts lead to the isolation of the same active principle in both extracts. The structure of the bioactive compound was identified via mass spectrometry and nuclear resonance spectroscopy and it turned out to be the known lipopeptide Lipid 430. We discovered and determined its previously unknown anti-bacterial activity against Streptococcus agalactiae and revealed a cytotoxic effect against the A2058 human melanoma cell line at significantly lower concentrations compared to its anti-bacterial concentration. Flow cytometry and microscopy investigations of the cytotoxicity against the melanoma cell line indicated that Lipid 430 did not cause immediate cell lysis. The experiments with melanoma cells suggest that the compound functions trough more complex pathways than acting as a simple detergent. Article in Journal/Newspaper Barents Sea University of Tromsø: Munin Open Research Archive Barents Sea Molecules 24 21 3991
institution Open Polar
collection University of Tromsø: Munin Open Research Archive
op_collection_id ftunivtroemsoe
language English
topic VDP::Matematikk og naturvitenskap: 400::Basale biofag: 470::Biokjemi: 476
VDP::Mathematics and natural scienses: 400::Basic biosciences: 470::Biochemistry: 476
spellingShingle VDP::Matematikk og naturvitenskap: 400::Basale biofag: 470::Biokjemi: 476
VDP::Mathematics and natural scienses: 400::Basic biosciences: 470::Biochemistry: 476
Schneider, Yannik Karl-Heinz
Østnes Hansen, Kine
Isaksson, Johan
Ullsten-Wahlund, Sara Maria
Hansen, Espen
Andersen, Jeanette Hammer
Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp.
topic_facet VDP::Matematikk og naturvitenskap: 400::Basale biofag: 470::Biokjemi: 476
VDP::Mathematics and natural scienses: 400::Basic biosciences: 470::Biochemistry: 476
description Two bacterial isolates from the Barents Sea, both belonging to the genus Algibacter , were found to yield extracts with anti-bacterial bioactivity. Mass spectrometry guided dereplication and purification of the active extracts lead to the isolation of the same active principle in both extracts. The structure of the bioactive compound was identified via mass spectrometry and nuclear resonance spectroscopy and it turned out to be the known lipopeptide Lipid 430. We discovered and determined its previously unknown anti-bacterial activity against Streptococcus agalactiae and revealed a cytotoxic effect against the A2058 human melanoma cell line at significantly lower concentrations compared to its anti-bacterial concentration. Flow cytometry and microscopy investigations of the cytotoxicity against the melanoma cell line indicated that Lipid 430 did not cause immediate cell lysis. The experiments with melanoma cells suggest that the compound functions trough more complex pathways than acting as a simple detergent.
format Article in Journal/Newspaper
author Schneider, Yannik Karl-Heinz
Østnes Hansen, Kine
Isaksson, Johan
Ullsten-Wahlund, Sara Maria
Hansen, Espen
Andersen, Jeanette Hammer
author_facet Schneider, Yannik Karl-Heinz
Østnes Hansen, Kine
Isaksson, Johan
Ullsten-Wahlund, Sara Maria
Hansen, Espen
Andersen, Jeanette Hammer
author_sort Schneider, Yannik Karl-Heinz
title Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp.
title_short Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp.
title_full Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp.
title_fullStr Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp.
title_full_unstemmed Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp.
title_sort anti-bacterial effect and cytotoxicity assessment of lipid 430 isolated from algibacter sp.
publisher MDPI
publishDate 2019
url https://hdl.handle.net/10037/16686
https://doi.org/10.3390/molecules24213991
geographic Barents Sea
geographic_facet Barents Sea
genre Barents Sea
genre_facet Barents Sea
op_relation Schneider, Y.K.-H. (2020). Bioactive secondary metabolites from bacteria. Natural products from marine and terrestrial bacteria, dereplication, isolation and investigation of bacterial secondary metabolites. (Doctoral thesis). https://hdl.handle.net/10037/19264
Molecules
info:eu-repo/grantAgreement/EC/H2020/721421/EU/Improving the flow in the pipeline of the next generation of marine biodiscovery scientists/MarPipe/
Schneider, Y.K.H., Hansen, K.Ø., Isaksson, J., Ullsten, S., Hansen, E.H. & Andersen, J.H. (2019). Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. Molecules, 24 (21), 3991. https://doi.org/10.3390/molecules24213991
FRIDAID 1744987
doi:10.3390/molecules24213991
1420-3049
https://hdl.handle.net/10037/16686
op_rights openAccess
op_doi https://doi.org/10.3390/molecules24213991
container_title Molecules
container_volume 24
container_issue 21
container_start_page 3991
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