Ocean acidification at a coastal CO2 vent induces expression of stress-related transcripts and transposable elements in the sea anemone Anemonia viridis
Published version, available at: https://doi.org/10.1371/journal.pone.0210358 Ocean acidification threatens to disrupt interactions between organisms throughout marine ecosystems. The diversity of reef-building organisms decreases as seawater CO2 increases along natural gradients, yet soft-bodied an...
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ftunivtroemsoe:oai:munin.uit.no:10037/15777 2023-05-15T17:50:04+02:00 Ocean acidification at a coastal CO2 vent induces expression of stress-related transcripts and transposable elements in the sea anemone Anemonia viridis Urbarova, Ilona Forêt, Sylvain Dahl, Mikael Per Emblem, Åse E. Milazzo, Marco Hall-Spencer, Jason M. Johansen, Steinar Daae 2019-05-08 https://hdl.handle.net/10037/15777 eng eng PLOS The RNA-seq raw sequencing data sets of twelve individuals of Anemonia viridis used in this study together with the transcriptome assembly are archived at NCBI’s Sequence Read Archive (SRA) under accession number PRJNA448978. The Transcriptome Shotgun Assembly (TSA) has been deposited at DDBJ/EMBL/GenBank under the accession GHCD00000000. The version described in this paper is the first version, GHCD01000000. PLoS ONE Urbarova, I., Forêt, S., Dahl, M.P., Emblem, Å.E., Milazzo, M., Hall-Spencer, J.M., Johansen, S.D. (2019) Ocean acidification at a coastal CO2 vent induces expression of stress-related transcripts and transposable elements in the sea anemone Anemonia viridis. PLoS ONE. 2019;14 , (5). https://doi.org/10.1371/journal.pone.0210358 FRIDAID 1703482 1932-6203 https://hdl.handle.net/10037/15777 openAccess VDP::Mathematics and natural science: 400::Basic biosciences: 470 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 Journal article Peer reviewed 2019 ftunivtroemsoe https://doi.org/10.1371/journal.pone.0210358 2021-06-25T17:56:38Z Published version, available at: https://doi.org/10.1371/journal.pone.0210358 Ocean acidification threatens to disrupt interactions between organisms throughout marine ecosystems. The diversity of reef-building organisms decreases as seawater CO2 increases along natural gradients, yet soft-bodied animals, such as sea anemones, are often resilient. We sequenced the polyA-enriched transcriptome of adult sea anemone Anemonia viridis and its dinoflagellate symbiont sampled along a natural CO2 gradient in Italy to assess stress levels in these organisms. We found that about 1.4% of the anemone transcripts, but only ~0.5% of the Symbiodinium sp. transcripts were differentially expressed. Processes enriched at high seawater CO2 were mainly linked to cellular stress, including significant up-regulation of protective cellular functions and deregulation of metabolic pathways. Transposable elements were differentially expressed at high seawater CO2, with an extreme up-regulation (> 100-fold) of the BEL-family of long terminal repeat retrotransposons. Seawater acidified by CO2 generated a significant stress reaction in A. viridis, but no bleaching was observed and Symbiodinium sp. appeared to be less affected. These observed changes indicate the mechanisms by which A. viridis acclimate to survive chronic exposure to ocean acidification conditions. We conclude that many organisms that are common in acidified conditions may nevertheless incur costs due to hypercapnia and/or lowered carbonate saturation states. Article in Journal/Newspaper Ocean acidification University of Tromsø: Munin Open Research Archive PLOS ONE 14 5 e0210358 |
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University of Tromsø: Munin Open Research Archive |
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ftunivtroemsoe |
language |
English |
topic |
VDP::Mathematics and natural science: 400::Basic biosciences: 470 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 |
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VDP::Mathematics and natural science: 400::Basic biosciences: 470 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 Urbarova, Ilona Forêt, Sylvain Dahl, Mikael Per Emblem, Åse E. Milazzo, Marco Hall-Spencer, Jason M. Johansen, Steinar Daae Ocean acidification at a coastal CO2 vent induces expression of stress-related transcripts and transposable elements in the sea anemone Anemonia viridis |
topic_facet |
VDP::Mathematics and natural science: 400::Basic biosciences: 470 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 |
description |
Published version, available at: https://doi.org/10.1371/journal.pone.0210358 Ocean acidification threatens to disrupt interactions between organisms throughout marine ecosystems. The diversity of reef-building organisms decreases as seawater CO2 increases along natural gradients, yet soft-bodied animals, such as sea anemones, are often resilient. We sequenced the polyA-enriched transcriptome of adult sea anemone Anemonia viridis and its dinoflagellate symbiont sampled along a natural CO2 gradient in Italy to assess stress levels in these organisms. We found that about 1.4% of the anemone transcripts, but only ~0.5% of the Symbiodinium sp. transcripts were differentially expressed. Processes enriched at high seawater CO2 were mainly linked to cellular stress, including significant up-regulation of protective cellular functions and deregulation of metabolic pathways. Transposable elements were differentially expressed at high seawater CO2, with an extreme up-regulation (> 100-fold) of the BEL-family of long terminal repeat retrotransposons. Seawater acidified by CO2 generated a significant stress reaction in A. viridis, but no bleaching was observed and Symbiodinium sp. appeared to be less affected. These observed changes indicate the mechanisms by which A. viridis acclimate to survive chronic exposure to ocean acidification conditions. We conclude that many organisms that are common in acidified conditions may nevertheless incur costs due to hypercapnia and/or lowered carbonate saturation states. |
format |
Article in Journal/Newspaper |
author |
Urbarova, Ilona Forêt, Sylvain Dahl, Mikael Per Emblem, Åse E. Milazzo, Marco Hall-Spencer, Jason M. Johansen, Steinar Daae |
author_facet |
Urbarova, Ilona Forêt, Sylvain Dahl, Mikael Per Emblem, Åse E. Milazzo, Marco Hall-Spencer, Jason M. Johansen, Steinar Daae |
author_sort |
Urbarova, Ilona |
title |
Ocean acidification at a coastal CO2 vent induces expression of stress-related transcripts and transposable elements in the sea anemone Anemonia viridis |
title_short |
Ocean acidification at a coastal CO2 vent induces expression of stress-related transcripts and transposable elements in the sea anemone Anemonia viridis |
title_full |
Ocean acidification at a coastal CO2 vent induces expression of stress-related transcripts and transposable elements in the sea anemone Anemonia viridis |
title_fullStr |
Ocean acidification at a coastal CO2 vent induces expression of stress-related transcripts and transposable elements in the sea anemone Anemonia viridis |
title_full_unstemmed |
Ocean acidification at a coastal CO2 vent induces expression of stress-related transcripts and transposable elements in the sea anemone Anemonia viridis |
title_sort |
ocean acidification at a coastal co2 vent induces expression of stress-related transcripts and transposable elements in the sea anemone anemonia viridis |
publisher |
PLOS |
publishDate |
2019 |
url |
https://hdl.handle.net/10037/15777 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
The RNA-seq raw sequencing data sets of twelve individuals of Anemonia viridis used in this study together with the transcriptome assembly are archived at NCBI’s Sequence Read Archive (SRA) under accession number PRJNA448978. The Transcriptome Shotgun Assembly (TSA) has been deposited at DDBJ/EMBL/GenBank under the accession GHCD00000000. The version described in this paper is the first version, GHCD01000000. PLoS ONE Urbarova, I., Forêt, S., Dahl, M.P., Emblem, Å.E., Milazzo, M., Hall-Spencer, J.M., Johansen, S.D. (2019) Ocean acidification at a coastal CO2 vent induces expression of stress-related transcripts and transposable elements in the sea anemone Anemonia viridis. PLoS ONE. 2019;14 , (5). https://doi.org/10.1371/journal.pone.0210358 FRIDAID 1703482 1932-6203 https://hdl.handle.net/10037/15777 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0210358 |
container_title |
PLOS ONE |
container_volume |
14 |
container_issue |
5 |
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e0210358 |
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