Characterization of the cecum microbiome from wild and captive rock ptarmigans indigenous to Arctic Norway
Source at https://doi.org/10.1371/journal.pone.0213503 . Rock ptarmigans ( Lagopus muta ) are gallinaceous birds inhabiting arctic and sub-arctic environments. Their diet varies by season, including plants or plant parts of high nutritional value, but also toxic plant secondary metabolites (PSMs). L...
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ftunivtroemsoe:oai:munin.uit.no:10037/15475 2023-05-15T14:27:22+02:00 Characterization of the cecum microbiome from wild and captive rock ptarmigans indigenous to Arctic Norway Salgado-Flores, Alejandro Tveit, Alexander Tøsdal Wright, A-D Pope, Phillip Sundset, Monica Alterskjær 2019-03-11 https://hdl.handle.net/10037/15475 https://doi.org/10.1371/journal.pone.0213503 eng eng Public Library of Science The sequence reads obtained from 16S amplicon sequencing and shotgun metagenomics are available at the Sequence Read Archive (SRA) database under the BioProject identifier PRJNA450906. PLoS ONE info:eu-repo/grantAgreement/RCN/FRIBIO2/250479/Norway/Back to basics: simplifying microbial communities to decrypt complex interactions// info:eu-repo/grantAgreement/RCN/FRIMEDBIO/251027/Norway/ Time & Energy: Fundamental microbial mechanisms that control CH4 dynamics in a warming Arctic// info:eu-repo/grantAgreement/EC/FP7/336355/EU/Interpreting the irrecoverable microbiota in digestive ecosystems/MICRODE/ info:eu-repo/grantAgreement/EC/FP7/608695/EU/ Marie Curie cofunding of the FRICON mobility programme in the Research Council of Norway scheme for independent basic research projects/FRICON/ Salgado-Flores, A., Tveit, A.T., Wright, A.-D., Pope, P.B. & Sundset, M.A. (2019). Characterization of the cecum microbiome from wild and captive rock ptarmigans indigenous to Arctic Norway. PLoS ONE, 14 (3), e0213503. https://doi.org/10.1371/journal.pone.0213503 FRIDAID 1685450 https://doi.org/10.1371/journal.pone.0213503 1932-6203 https://hdl.handle.net/10037/15475 openAccess Journal article Tidsskriftartikkel Peer reviewed 2019 ftunivtroemsoe https://doi.org/10.1371/journal.pone.0213503 2021-06-25T17:56:30Z Source at https://doi.org/10.1371/journal.pone.0213503 . Rock ptarmigans ( Lagopus muta ) are gallinaceous birds inhabiting arctic and sub-arctic environments. Their diet varies by season, including plants or plant parts of high nutritional value, but also toxic plant secondary metabolites (PSMs). Little is known about the microbes driving organic matter decomposition in the cecum of ptarmigans, especially the last steps leading to methanogenesis. The cecum microbiome in wild rock ptarmigans from Arctic Norway was characterized to unveil their functional potential for PSM detoxification, methanogenesis and polysaccharides degradation. Cecal samples were collected from wild ptarmigans from Svalbard ( L. m. hyperborea ) and northern Norway ( L. m. muta ) during autumn/winter (Sept-Dec). Samples from captive Svalbard ptarmigans fed commercial pelleted feed were included to investigate the effect of diet on microbial composition and function. Abundances of methanogens and bacteria were determined by qRT-PCR, while microbial community composition and functional potential were studied using 16S rRNA gene sequencing and shotgun metagenomics. Abundances of bacteria and methanogenic Archaea were higher in wild ptarmigans compared to captive birds. The ceca of wild ptarmigans housed bacterial groups involved in PSM-degradation, and genes mediating the conversion of phenol compounds to pyruvate. Methanomassiliicoccaceae was the major archaeal family in wild ptarmigans, carrying the genes for methanogenesis from methanol. It might be related to increased methanol production from pectin degradation in wild birds due to a diet consisting of primarily fresh pectin-rich plants. Both wild and captive ptarmigans possessed a broad suite of genes for the depolymerization of hemicellulose and non-cellulosic polysaccharides (e.g. starch). In conclusion, there were no physiological and phenotypical dissimilarities in the microbiota found in the cecum of wild ptarmigans on mainland Norway and Svalbard. While substantial differences in the functional potential for PSM degradation and methanogenesis in wild and captive birds seem to be a direct consequence of their dissimilar diets. Article in Journal/Newspaper Arctic Arctic Lagopus muta Northern Norway Svalbard University of Tromsø: Munin Open Research Archive Arctic Norway Svalbard PLOS ONE 14 3 e0213503 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
description |
Source at https://doi.org/10.1371/journal.pone.0213503 . Rock ptarmigans ( Lagopus muta ) are gallinaceous birds inhabiting arctic and sub-arctic environments. Their diet varies by season, including plants or plant parts of high nutritional value, but also toxic plant secondary metabolites (PSMs). Little is known about the microbes driving organic matter decomposition in the cecum of ptarmigans, especially the last steps leading to methanogenesis. The cecum microbiome in wild rock ptarmigans from Arctic Norway was characterized to unveil their functional potential for PSM detoxification, methanogenesis and polysaccharides degradation. Cecal samples were collected from wild ptarmigans from Svalbard ( L. m. hyperborea ) and northern Norway ( L. m. muta ) during autumn/winter (Sept-Dec). Samples from captive Svalbard ptarmigans fed commercial pelleted feed were included to investigate the effect of diet on microbial composition and function. Abundances of methanogens and bacteria were determined by qRT-PCR, while microbial community composition and functional potential were studied using 16S rRNA gene sequencing and shotgun metagenomics. Abundances of bacteria and methanogenic Archaea were higher in wild ptarmigans compared to captive birds. The ceca of wild ptarmigans housed bacterial groups involved in PSM-degradation, and genes mediating the conversion of phenol compounds to pyruvate. Methanomassiliicoccaceae was the major archaeal family in wild ptarmigans, carrying the genes for methanogenesis from methanol. It might be related to increased methanol production from pectin degradation in wild birds due to a diet consisting of primarily fresh pectin-rich plants. Both wild and captive ptarmigans possessed a broad suite of genes for the depolymerization of hemicellulose and non-cellulosic polysaccharides (e.g. starch). In conclusion, there were no physiological and phenotypical dissimilarities in the microbiota found in the cecum of wild ptarmigans on mainland Norway and Svalbard. While substantial differences in the functional potential for PSM degradation and methanogenesis in wild and captive birds seem to be a direct consequence of their dissimilar diets. |
format |
Article in Journal/Newspaper |
author |
Salgado-Flores, Alejandro Tveit, Alexander Tøsdal Wright, A-D Pope, Phillip Sundset, Monica Alterskjær |
spellingShingle |
Salgado-Flores, Alejandro Tveit, Alexander Tøsdal Wright, A-D Pope, Phillip Sundset, Monica Alterskjær Characterization of the cecum microbiome from wild and captive rock ptarmigans indigenous to Arctic Norway |
author_facet |
Salgado-Flores, Alejandro Tveit, Alexander Tøsdal Wright, A-D Pope, Phillip Sundset, Monica Alterskjær |
author_sort |
Salgado-Flores, Alejandro |
title |
Characterization of the cecum microbiome from wild and captive rock ptarmigans indigenous to Arctic Norway |
title_short |
Characterization of the cecum microbiome from wild and captive rock ptarmigans indigenous to Arctic Norway |
title_full |
Characterization of the cecum microbiome from wild and captive rock ptarmigans indigenous to Arctic Norway |
title_fullStr |
Characterization of the cecum microbiome from wild and captive rock ptarmigans indigenous to Arctic Norway |
title_full_unstemmed |
Characterization of the cecum microbiome from wild and captive rock ptarmigans indigenous to Arctic Norway |
title_sort |
characterization of the cecum microbiome from wild and captive rock ptarmigans indigenous to arctic norway |
publisher |
Public Library of Science |
publishDate |
2019 |
url |
https://hdl.handle.net/10037/15475 https://doi.org/10.1371/journal.pone.0213503 |
geographic |
Arctic Norway Svalbard |
geographic_facet |
Arctic Norway Svalbard |
genre |
Arctic Arctic Lagopus muta Northern Norway Svalbard |
genre_facet |
Arctic Arctic Lagopus muta Northern Norway Svalbard |
op_relation |
The sequence reads obtained from 16S amplicon sequencing and shotgun metagenomics are available at the Sequence Read Archive (SRA) database under the BioProject identifier PRJNA450906. PLoS ONE info:eu-repo/grantAgreement/RCN/FRIBIO2/250479/Norway/Back to basics: simplifying microbial communities to decrypt complex interactions// info:eu-repo/grantAgreement/RCN/FRIMEDBIO/251027/Norway/ Time & Energy: Fundamental microbial mechanisms that control CH4 dynamics in a warming Arctic// info:eu-repo/grantAgreement/EC/FP7/336355/EU/Interpreting the irrecoverable microbiota in digestive ecosystems/MICRODE/ info:eu-repo/grantAgreement/EC/FP7/608695/EU/ Marie Curie cofunding of the FRICON mobility programme in the Research Council of Norway scheme for independent basic research projects/FRICON/ Salgado-Flores, A., Tveit, A.T., Wright, A.-D., Pope, P.B. & Sundset, M.A. (2019). Characterization of the cecum microbiome from wild and captive rock ptarmigans indigenous to Arctic Norway. PLoS ONE, 14 (3), e0213503. https://doi.org/10.1371/journal.pone.0213503 FRIDAID 1685450 https://doi.org/10.1371/journal.pone.0213503 1932-6203 https://hdl.handle.net/10037/15475 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0213503 |
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PLOS ONE |
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14 |
container_issue |
3 |
container_start_page |
e0213503 |
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