Impact of Multiple Ecological Stressors on a Sub-Arctic Ecosystem: No Interaction Between Extreme Winter Warming Events, Nitrogen Addition and Grazing

• Published in: Frontiers in Plant Science
• Main Authors: Bokhorst, Stef, Berg, Matty P., Edvinsen, Guro Kristine, Ellers, Jacintha, Heitman, Amber, Jaakola, Laura, Mæhre, Hanne K, Phoenix, Gareth K., Tømmervik, Hans, Bjerke, Jarle W.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Frontiers Media 2018
• Subjects: VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920; VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920; VDP::Mathematics and natural science: 400::Zoology and botany: 480; VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480; cryptogam; CO2 fluxes; fatty acids; frost; geometrid moth; herbivory; multiple stress; snow; Arctic; Norway; Climate change; Empetrum nigrum; Northern Norway
• Online Access: https://hdl.handle.net/10037/14915; https://doi.org/10.3389/fpls.2018.01787

The following article, Bokhorst, S., Berg, M.P., Edvinsen, G.K., Ellers, J., Heitman, A., Jaakola, L., . Bjerke, J.W. (2018). Impact of Multiple Ecological Stressors on a Sub-Arctic Ecosystem: No Interaction Between Extreme Winter Warming Events, Nitrogen Addition and Grazing. Frontiers in Plant Science, 9 , can be accessed at https://doi.org/10.3389/fpls.2018.01787 . Climate change is one of many ongoing human-induced environmental changes, but few studies consider interactive effects between multiple anthropogenic disturbances. In coastal sub-arctic heathland, we quantified the impact of a factorial design simulating extreme winter warming (WW) events (7 days at 6–7∘C) combined with episodic summer nitrogen (+N) depositions (5 kg N ha-1) on plant winter physiology, plant community composition and ecosystem CO2 fluxes of an Empetrum nigrum dominated heathland during 3 consecutive years in northern Norway. We expected that the +N would exacerbate any stress effects caused by the WW treatment. During WW events, ecosystem respiration doubled, leaf respiration declined (-58%), efficiency of Photosystem II (Fv/Fm) increased (between 26 and 88%), while cell membrane fatty acids showed strong compositional changes as a result of the warming and freezing. In particular, longer fatty acid chains increased as a result of WW events, and eicosadienoic acid (C20:2) was lower when plants were exposed to the combination of WW and +N. A larval outbreak of geometrid moths ( Epirrita autumnata and Operophtera brumata ) following the first WW led to a near-complete leaf defoliation of the dominant dwarf shrubs E. nigrum (-87%) and Vaccinium myrtillus (-81%) across all experimental plots. Leaf emergence timing, plant biomass or composition, NDVI and growing season ecosystem CO2 fluxes were unresponsive to the WW and +N treatments. The limited plant community response reflected the relative mild winter freezing temperatures (-6.6∘C to -11.8∘C) recorded after the WW events, and that the grazing pressure probably overshadowed any potential treatment effects. The grazing pressure and WW both induce damage to the evergreen shrubs and their combination should therefore be even stronger. In addition, +N could have exacerbated the impact of both extreme events, but the ecosystem responses did not support this. Therefore, our results indicate that these sub-arctic Empetrum -dominated ecosystems are highly resilient and that their responses may be limited to the event with the strongest impact.