Transcriptome analyses of Atlantic salmon muscle genes induced by a DNA vaccine against salmonid alphavirus, the causative agent of salmon pancreas disease (PD)
The following article, Sobhkhez, M., Krasnov, A. & Robertsen, B. (2018). Transcriptome analyses of Atlantic salmon muscle genes induced by a DNA vaccine against salmonid alphavirus, the causative agent of salmon pancreas disease (PD). PLoS ONE , 13:e0204924(10). https://doi.org/10.1371/journal.p...
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Online Access: | https://hdl.handle.net/10037/14152 https://doi.org/10.1371/journal.pone.0204924 |
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ftunivtroemsoe:oai:munin.uit.no:10037/14152 2023-05-15T15:31:14+02:00 Transcriptome analyses of Atlantic salmon muscle genes induced by a DNA vaccine against salmonid alphavirus, the causative agent of salmon pancreas disease (PD) Sobhkhez, Mehrdad Krasnov, Aleksei Robertsen, Børre 2018-10-01 https://hdl.handle.net/10037/14152 https://doi.org/10.1371/journal.pone.0204924 eng eng Public Library of Science PLoS ONE info:eu-repo/grantAgreement/RCN/HAVBRUK2/254907/Norway/The road to successful DNA vaccination of Atlantic salmon against virus diseases// Sobhkhez, M., Krasnov, A. & Robertsen, B. (2018). Transcriptome analyses of Atlantic salmon muscle genes induced by a DNA vaccine against salmonid alphavirus, the causative agent of salmon pancreas disease (PD). PLoS ONE , 13:e0204924(10). https://doi.org/10.1371/journal.pone.0204924 FRIDAID 1622298 doi:10.1371/journal.pone.0204924 1932-6203 https://hdl.handle.net/10037/14152 openAccess VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 Journal article Tidsskriftartikkel Peer reviewed 2018 ftunivtroemsoe https://doi.org/10.1371/journal.pone.0204924 2021-06-25T17:56:12Z The following article, Sobhkhez, M., Krasnov, A. & Robertsen, B. (2018). Transcriptome analyses of Atlantic salmon muscle genes induced by a DNA vaccine against salmonid alphavirus, the causative agent of salmon pancreas disease (PD). PLoS ONE , 13:e0204924(10). https://doi.org/10.1371/journal.pone.0204924, can be accessed at https://doi.org/10.1371/journal.pone.0204924 . Salmonid alphavirus (SAV) is the causative agent of pancreas disease (PD) in farmed Atlantic salmon. A previous study showed that vaccination of pre-smolt salmon with a plasmid encoding the structural polypeptide of SAV gave protection against infection and development of PD accompanied by production of antibodies against the virus. In the present work we analyzed transcript responses in the muscle to vaccination with this plasmid (here named pSAV). The purpose was to shed light on how pSAV might initiate adaptive immune responses in the fish. The work was based on microarray and reverse transcription quantitative PCR analyses of muscle at the injection site 7 days after vaccination. The results showed that pSAV and pcDNA3.3 had similar abilities to up-regulate type I IFN stimulated genes. In contrast, pSAV caused higher up-regulation of IFNγ and several IFNγ inducible genes. Compared to pcDNA3.3, pSAV also gave larger increase in transcripts of marker genes for B-cells, T-cells and antigen presenting cells (APCs), which suggest attraction and role of these cells in the adaptive immune responses elicited by pSAV. Moreover, pSAV caused a stronger up-regulation of the chemokine CXCL10 and the proinflammatory cytokines IL-1ß and TNFα, which may explain attraction of lymphocytes and APCs. The present work shows that the expression profile of genes resulting from vaccination with pSAV is different from the expression profiles obtained previously by vaccination of salmonids with DNA vaccines against infectious salmon anemia virus and infectious hematopoietic necrosis virus. Article in Journal/Newspaper Atlantic salmon University of Tromsø: Munin Open Research Archive Sav’ ENVELOPE(156.400,156.400,68.817,68.817) PLOS ONE 13 10 e0204924 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 |
spellingShingle |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 Sobhkhez, Mehrdad Krasnov, Aleksei Robertsen, Børre Transcriptome analyses of Atlantic salmon muscle genes induced by a DNA vaccine against salmonid alphavirus, the causative agent of salmon pancreas disease (PD) |
topic_facet |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 |
description |
The following article, Sobhkhez, M., Krasnov, A. & Robertsen, B. (2018). Transcriptome analyses of Atlantic salmon muscle genes induced by a DNA vaccine against salmonid alphavirus, the causative agent of salmon pancreas disease (PD). PLoS ONE , 13:e0204924(10). https://doi.org/10.1371/journal.pone.0204924, can be accessed at https://doi.org/10.1371/journal.pone.0204924 . Salmonid alphavirus (SAV) is the causative agent of pancreas disease (PD) in farmed Atlantic salmon. A previous study showed that vaccination of pre-smolt salmon with a plasmid encoding the structural polypeptide of SAV gave protection against infection and development of PD accompanied by production of antibodies against the virus. In the present work we analyzed transcript responses in the muscle to vaccination with this plasmid (here named pSAV). The purpose was to shed light on how pSAV might initiate adaptive immune responses in the fish. The work was based on microarray and reverse transcription quantitative PCR analyses of muscle at the injection site 7 days after vaccination. The results showed that pSAV and pcDNA3.3 had similar abilities to up-regulate type I IFN stimulated genes. In contrast, pSAV caused higher up-regulation of IFNγ and several IFNγ inducible genes. Compared to pcDNA3.3, pSAV also gave larger increase in transcripts of marker genes for B-cells, T-cells and antigen presenting cells (APCs), which suggest attraction and role of these cells in the adaptive immune responses elicited by pSAV. Moreover, pSAV caused a stronger up-regulation of the chemokine CXCL10 and the proinflammatory cytokines IL-1ß and TNFα, which may explain attraction of lymphocytes and APCs. The present work shows that the expression profile of genes resulting from vaccination with pSAV is different from the expression profiles obtained previously by vaccination of salmonids with DNA vaccines against infectious salmon anemia virus and infectious hematopoietic necrosis virus. |
format |
Article in Journal/Newspaper |
author |
Sobhkhez, Mehrdad Krasnov, Aleksei Robertsen, Børre |
author_facet |
Sobhkhez, Mehrdad Krasnov, Aleksei Robertsen, Børre |
author_sort |
Sobhkhez, Mehrdad |
title |
Transcriptome analyses of Atlantic salmon muscle genes induced by a DNA vaccine against salmonid alphavirus, the causative agent of salmon pancreas disease (PD) |
title_short |
Transcriptome analyses of Atlantic salmon muscle genes induced by a DNA vaccine against salmonid alphavirus, the causative agent of salmon pancreas disease (PD) |
title_full |
Transcriptome analyses of Atlantic salmon muscle genes induced by a DNA vaccine against salmonid alphavirus, the causative agent of salmon pancreas disease (PD) |
title_fullStr |
Transcriptome analyses of Atlantic salmon muscle genes induced by a DNA vaccine against salmonid alphavirus, the causative agent of salmon pancreas disease (PD) |
title_full_unstemmed |
Transcriptome analyses of Atlantic salmon muscle genes induced by a DNA vaccine against salmonid alphavirus, the causative agent of salmon pancreas disease (PD) |
title_sort |
transcriptome analyses of atlantic salmon muscle genes induced by a dna vaccine against salmonid alphavirus, the causative agent of salmon pancreas disease (pd) |
publisher |
Public Library of Science |
publishDate |
2018 |
url |
https://hdl.handle.net/10037/14152 https://doi.org/10.1371/journal.pone.0204924 |
long_lat |
ENVELOPE(156.400,156.400,68.817,68.817) |
geographic |
Sav’ |
geographic_facet |
Sav’ |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
PLoS ONE info:eu-repo/grantAgreement/RCN/HAVBRUK2/254907/Norway/The road to successful DNA vaccination of Atlantic salmon against virus diseases// Sobhkhez, M., Krasnov, A. & Robertsen, B. (2018). Transcriptome analyses of Atlantic salmon muscle genes induced by a DNA vaccine against salmonid alphavirus, the causative agent of salmon pancreas disease (PD). PLoS ONE , 13:e0204924(10). https://doi.org/10.1371/journal.pone.0204924 FRIDAID 1622298 doi:10.1371/journal.pone.0204924 1932-6203 https://hdl.handle.net/10037/14152 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0204924 |
container_title |
PLOS ONE |
container_volume |
13 |
container_issue |
10 |
container_start_page |
e0204924 |
_version_ |
1766361736652259328 |