Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group
The marine environment is a rich source of biodiversity, including microorganisms that have proven to be prolific producers of bioactive secondary metabolites. Arctic seas are less explored than warmer, more accessible areas, providing a promising starting point to search for novel bioactive compoun...
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Online Access: | https://hdl.handle.net/10037/13600 https://doi.org/10.3390/md16050163 |
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ftunivtroemsoe:oai:munin.uit.no:10037/13600 2023-05-15T14:25:11+02:00 Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group Kristoffersen, Venke Rämä, Teppo Isaksson, Johan Andersen, Jeanette H. Gerwick, William H. Hansen, Espen 2018-05-14 https://hdl.handle.net/10037/13600 https://doi.org/10.3390/md16050163 eng eng MDPI Kristoffersen, V. (2021). Bioprospecting of Arctic marine microorganisms. Exploring microbial secondary metabolite production using the one strain-many compounds approach: isolation and characterization of secondary metabolites. (Doctoral thesis). https://hdl.handle.net/10037/23299 Marine Drugs info:eu-repo/grantAgreement/EC/FP7-KBBE/312184/EU/Increasing Value and Flow in the Marine Biodiscovery Pipeline/PHARMASEA/ Kristoffersen, V., Rämä, T., Isaksson, J., Andersen, J.H., Gerwick, W.H: & Hansen, E. (2018). Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group. Marine Drugs, 16(5), 163. https://doi.org/10.3390/md16050163. FRIDAID 1584973 doi:10.3390/md16050163 1660-3397 https://hdl.handle.net/10037/13600 openAccess VDP::Technology: 500::Biotechnology: 590 VDP::Teknologi: 500::Bioteknologi: 590 Arctic bacteria Bioactive OSMAC (one strain many compounds) Molecular networking Rhamnolipids Journal article Tidsskriftartikkel Peer reviewed 2018 ftunivtroemsoe https://doi.org/10.3390/md16050163 2021-12-08T23:55:19Z The marine environment is a rich source of biodiversity, including microorganisms that have proven to be prolific producers of bioactive secondary metabolites. Arctic seas are less explored than warmer, more accessible areas, providing a promising starting point to search for novel bioactive compounds. In the present work, an Arctic marine Pseudomonas sp. belonging to the Pseudomonas (P.) fluorescence group was cultivated in four different media in an attempt to activate biosynthetic pathways leading to the production of antibacterial and anticancer compounds. Culture extracts were pre-fractionated and screened for antibacterial and anticancer activities. One fraction from three of the four growth conditions showed inhibitory activity towards bacteria and cancer cells. The active fractions were dereplicated using molecular networking based on MS/MS fragmentation data, indicating the presence of a cluster of related rhamnolipids. Six compounds were isolated using HPLC and mass-guided fractionation, and by interpreting data from NMR and high-resolution MS/MS analysis; the structures of the compounds were determined to be five mono-rhamnolipids and the lipid moiety of one of the rhamnolipids. Molecular networking proved to be a valuable tool for dereplication of these related compounds, and for the first time, five mono-rhamnolipids from a bacterium within the P. fluorescence group were characterized, including one new mono-rhamnolipid. Article in Journal/Newspaper Arctic Arctic University of Tromsø: Munin Open Research Archive Arctic Marine Drugs 16 5 163 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Technology: 500::Biotechnology: 590 VDP::Teknologi: 500::Bioteknologi: 590 Arctic bacteria Bioactive OSMAC (one strain many compounds) Molecular networking Rhamnolipids |
spellingShingle |
VDP::Technology: 500::Biotechnology: 590 VDP::Teknologi: 500::Bioteknologi: 590 Arctic bacteria Bioactive OSMAC (one strain many compounds) Molecular networking Rhamnolipids Kristoffersen, Venke Rämä, Teppo Isaksson, Johan Andersen, Jeanette H. Gerwick, William H. Hansen, Espen Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group |
topic_facet |
VDP::Technology: 500::Biotechnology: 590 VDP::Teknologi: 500::Bioteknologi: 590 Arctic bacteria Bioactive OSMAC (one strain many compounds) Molecular networking Rhamnolipids |
description |
The marine environment is a rich source of biodiversity, including microorganisms that have proven to be prolific producers of bioactive secondary metabolites. Arctic seas are less explored than warmer, more accessible areas, providing a promising starting point to search for novel bioactive compounds. In the present work, an Arctic marine Pseudomonas sp. belonging to the Pseudomonas (P.) fluorescence group was cultivated in four different media in an attempt to activate biosynthetic pathways leading to the production of antibacterial and anticancer compounds. Culture extracts were pre-fractionated and screened for antibacterial and anticancer activities. One fraction from three of the four growth conditions showed inhibitory activity towards bacteria and cancer cells. The active fractions were dereplicated using molecular networking based on MS/MS fragmentation data, indicating the presence of a cluster of related rhamnolipids. Six compounds were isolated using HPLC and mass-guided fractionation, and by interpreting data from NMR and high-resolution MS/MS analysis; the structures of the compounds were determined to be five mono-rhamnolipids and the lipid moiety of one of the rhamnolipids. Molecular networking proved to be a valuable tool for dereplication of these related compounds, and for the first time, five mono-rhamnolipids from a bacterium within the P. fluorescence group were characterized, including one new mono-rhamnolipid. |
format |
Article in Journal/Newspaper |
author |
Kristoffersen, Venke Rämä, Teppo Isaksson, Johan Andersen, Jeanette H. Gerwick, William H. Hansen, Espen |
author_facet |
Kristoffersen, Venke Rämä, Teppo Isaksson, Johan Andersen, Jeanette H. Gerwick, William H. Hansen, Espen |
author_sort |
Kristoffersen, Venke |
title |
Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group |
title_short |
Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group |
title_full |
Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group |
title_fullStr |
Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group |
title_full_unstemmed |
Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group |
title_sort |
characterization of rhamnolipids produced by an arctic marine bacterium from the pseudomonas fluorescence group |
publisher |
MDPI |
publishDate |
2018 |
url |
https://hdl.handle.net/10037/13600 https://doi.org/10.3390/md16050163 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Arctic |
genre_facet |
Arctic Arctic |
op_relation |
Kristoffersen, V. (2021). Bioprospecting of Arctic marine microorganisms. Exploring microbial secondary metabolite production using the one strain-many compounds approach: isolation and characterization of secondary metabolites. (Doctoral thesis). https://hdl.handle.net/10037/23299 Marine Drugs info:eu-repo/grantAgreement/EC/FP7-KBBE/312184/EU/Increasing Value and Flow in the Marine Biodiscovery Pipeline/PHARMASEA/ Kristoffersen, V., Rämä, T., Isaksson, J., Andersen, J.H., Gerwick, W.H: & Hansen, E. (2018). Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group. Marine Drugs, 16(5), 163. https://doi.org/10.3390/md16050163. FRIDAID 1584973 doi:10.3390/md16050163 1660-3397 https://hdl.handle.net/10037/13600 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.3390/md16050163 |
container_title |
Marine Drugs |
container_volume |
16 |
container_issue |
5 |
container_start_page |
163 |
_version_ |
1766297600793772032 |