New Species of Saprobic Labyrinthulea (=Labyrinthulomycota) and the Erection of a gen. nov. to Resolve Molecular Polyphyly within the Aplanochytrids
This is the peer reviewed version of the following article: Hassett, B.T. & Gradinger, R. (2017). New Species of Saprobic Labyrinthulea (=Labyrinthulomycota) and the Erection of a gen. nov. to Resolve Molecular Polyphyly within the Aplanochytrids. Journal of Eukaryotic Microbiology, 65(4), 475-4...
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Online Access: | https://hdl.handle.net/10037/13570 https://doi.org/10.1111/jeu.12494 |
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ftunivtroemsoe:oai:munin.uit.no:10037/13570 2023-05-15T17:47:07+02:00 New Species of Saprobic Labyrinthulea (=Labyrinthulomycota) and the Erection of a gen. nov. to Resolve Molecular Polyphyly within the Aplanochytrids Hassett, Brandon Thomas Gradinger, Rolf 2017-12-19 https://hdl.handle.net/10037/13570 https://doi.org/10.1111/jeu.12494 eng eng Wiley Journal of Eukaryotic Microbiology Hassett, B.T. & Gradinger, R. (2017). New Species of Saprobic Labyrinthulea (=Labyrinthulomycota) and the Erection of a gen. nov. to Resolve Molecular Polyphyly within the Aplanochytrids. Journal of Eukaryotic Microbiology, 65(4), 475-483. https://doi.org/10.1111/jeu.12494 FRIDAID 1530747 doi:10.1111/jeu.12494 1066-5234 1550-7408 https://hdl.handle.net/10037/13570 openAccess VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Marine biology: 497 18S rRNA homoplasia Norwegian Sea pollen baiting thraustochytrid Journal article Tidsskriftartikkel Peer reviewed 2017 ftunivtroemsoe https://doi.org/10.1111/jeu.12494 2021-06-25T17:55:36Z This is the peer reviewed version of the following article: Hassett, B.T. & Gradinger, R. (2017). New Species of Saprobic Labyrinthulea (=Labyrinthulomycota) and the Erection of a gen. nov. to Resolve Molecular Polyphyly within the Aplanochytrids. Journal of Eukaryotic Microbiology, 65(4), 475-483. https://doi.org/10.1111/jeu.12494, which has been published in final form at https://doi.org/10.1111/jeu.12494 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. A culture of a unicellular heterotrophic eukaryote was established from pollen‐baited seawater acquired from the nearshore environment in Tromsø, Norway. Light microscopy revealed the production of ectoplasmic nets and reproduction by biflagellated zoospores, as well as binary division. After culturing and subsequent nucleotide extraction, database queries of the isolate's 18S small ribosomal subunit coding region identified closest molecular affinity to Aplanochytrium haliotidis , a pathogen of abalone. Testing of phylogenetic hypotheses consistently grouped our unknown isolate and A. haliotidis among the homoplasious thraustochytrids. Transmission electron microscopy revealed complex cell walls comprised of electron‐dense lamella that formed protuberances, some associated with bothrosomes. Co‐culturing experiments with the marine fungus Penicillium brevicompactum revealed prolonged interactions with hyphal strands. Based on the combined information acquired from electron microscopy, life history information, and phylogenetic testing, we describe our unknown isolate as a novel species. To resolve molecular polyphyly within the aplanochytrids, we erect a gen. nov. that circumscribes our novel isolate and the former A. haliotidis within the thraustochytrids. Article in Journal/Newspaper Norwegian Sea Tromsø University of Tromsø: Munin Open Research Archive Norway Norwegian Sea Tromsø Journal of Eukaryotic Microbiology 65 4 475 483 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Marine biology: 497 18S rRNA homoplasia Norwegian Sea pollen baiting thraustochytrid |
spellingShingle |
VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Marine biology: 497 18S rRNA homoplasia Norwegian Sea pollen baiting thraustochytrid Hassett, Brandon Thomas Gradinger, Rolf New Species of Saprobic Labyrinthulea (=Labyrinthulomycota) and the Erection of a gen. nov. to Resolve Molecular Polyphyly within the Aplanochytrids |
topic_facet |
VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Marine biology: 497 18S rRNA homoplasia Norwegian Sea pollen baiting thraustochytrid |
description |
This is the peer reviewed version of the following article: Hassett, B.T. & Gradinger, R. (2017). New Species of Saprobic Labyrinthulea (=Labyrinthulomycota) and the Erection of a gen. nov. to Resolve Molecular Polyphyly within the Aplanochytrids. Journal of Eukaryotic Microbiology, 65(4), 475-483. https://doi.org/10.1111/jeu.12494, which has been published in final form at https://doi.org/10.1111/jeu.12494 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. A culture of a unicellular heterotrophic eukaryote was established from pollen‐baited seawater acquired from the nearshore environment in Tromsø, Norway. Light microscopy revealed the production of ectoplasmic nets and reproduction by biflagellated zoospores, as well as binary division. After culturing and subsequent nucleotide extraction, database queries of the isolate's 18S small ribosomal subunit coding region identified closest molecular affinity to Aplanochytrium haliotidis , a pathogen of abalone. Testing of phylogenetic hypotheses consistently grouped our unknown isolate and A. haliotidis among the homoplasious thraustochytrids. Transmission electron microscopy revealed complex cell walls comprised of electron‐dense lamella that formed protuberances, some associated with bothrosomes. Co‐culturing experiments with the marine fungus Penicillium brevicompactum revealed prolonged interactions with hyphal strands. Based on the combined information acquired from electron microscopy, life history information, and phylogenetic testing, we describe our unknown isolate as a novel species. To resolve molecular polyphyly within the aplanochytrids, we erect a gen. nov. that circumscribes our novel isolate and the former A. haliotidis within the thraustochytrids. |
format |
Article in Journal/Newspaper |
author |
Hassett, Brandon Thomas Gradinger, Rolf |
author_facet |
Hassett, Brandon Thomas Gradinger, Rolf |
author_sort |
Hassett, Brandon Thomas |
title |
New Species of Saprobic Labyrinthulea (=Labyrinthulomycota) and the Erection of a gen. nov. to Resolve Molecular Polyphyly within the Aplanochytrids |
title_short |
New Species of Saprobic Labyrinthulea (=Labyrinthulomycota) and the Erection of a gen. nov. to Resolve Molecular Polyphyly within the Aplanochytrids |
title_full |
New Species of Saprobic Labyrinthulea (=Labyrinthulomycota) and the Erection of a gen. nov. to Resolve Molecular Polyphyly within the Aplanochytrids |
title_fullStr |
New Species of Saprobic Labyrinthulea (=Labyrinthulomycota) and the Erection of a gen. nov. to Resolve Molecular Polyphyly within the Aplanochytrids |
title_full_unstemmed |
New Species of Saprobic Labyrinthulea (=Labyrinthulomycota) and the Erection of a gen. nov. to Resolve Molecular Polyphyly within the Aplanochytrids |
title_sort |
new species of saprobic labyrinthulea (=labyrinthulomycota) and the erection of a gen. nov. to resolve molecular polyphyly within the aplanochytrids |
publisher |
Wiley |
publishDate |
2017 |
url |
https://hdl.handle.net/10037/13570 https://doi.org/10.1111/jeu.12494 |
geographic |
Norway Norwegian Sea Tromsø |
geographic_facet |
Norway Norwegian Sea Tromsø |
genre |
Norwegian Sea Tromsø |
genre_facet |
Norwegian Sea Tromsø |
op_relation |
Journal of Eukaryotic Microbiology Hassett, B.T. & Gradinger, R. (2017). New Species of Saprobic Labyrinthulea (=Labyrinthulomycota) and the Erection of a gen. nov. to Resolve Molecular Polyphyly within the Aplanochytrids. Journal of Eukaryotic Microbiology, 65(4), 475-483. https://doi.org/10.1111/jeu.12494 FRIDAID 1530747 doi:10.1111/jeu.12494 1066-5234 1550-7408 https://hdl.handle.net/10037/13570 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1111/jeu.12494 |
container_title |
Journal of Eukaryotic Microbiology |
container_volume |
65 |
container_issue |
4 |
container_start_page |
475 |
op_container_end_page |
483 |
_version_ |
1766151449232801792 |