Studies on the effects of LPS, ß‐glucan and metabolic inhibitors on the respiratory burst and gene expression in Atlantic salmon macrophages
This is the peer reviewed version of the following article: Ulvestad, J.S., Kumari, K., Seternes, T., Chi, H. & Dalmo, R.A. (2018). Studies on the effects of LPS, ß‐glucan and metabolic inhibitors on the respiratory burst and gene expression in Atlantic salmon macrophages. Journal of Fish Diseas...
Published in: | Journal of Fish Diseases |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2018
|
Subjects: | |
Online Access: | https://hdl.handle.net/10037/13497 |
id |
ftunivtroemsoe:oai:munin.uit.no:10037/13497 |
---|---|
record_format |
openpolar |
spelling |
ftunivtroemsoe:oai:munin.uit.no:10037/13497 2023-05-15T15:31:05+02:00 Studies on the effects of LPS, ß‐glucan and metabolic inhibitors on the respiratory burst and gene expression in Atlantic salmon macrophages Ulvestad, Johanne Skår Kumari, Jaya Seternes, Tore Chi, Heng Dalmo, Roy Ambli 2018-03-30 https://hdl.handle.net/10037/13497 eng eng Wiley Journal of Fish Diseases info:eu-repo/grantAgreement/RCN/HAVBRUK2/237315/Norway/Fish Virus Vaccines/ViVaFish/ info:eu-repo/grantAgreement/RCN/NANO2021/239140/Norway/Nanoparticle encapsulation of plant-based vaccine against piscine reovirus infection in salmon// Ulvestad, J.S., Kumari, K., Seternes, T., Chi, H. & Dalmo, R.A. (2018). Studies on the effects of LPS, ß‐glucan and metabolic inhibitors on the respiratory burst and gene expression in Atlantic salmon macrophages. Journal of Fish Diseases, 41(7), 1117-1127. https://doi.org/10.1111/jfd.12806 FRIDAID 1576941 0140-7775 1365-2761 https://hdl.handle.net/10037/13497 openAccess VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 gene expression macrophages metabolism respiratory burst salmon Journal article Tidsskriftartikkel Peer reviewed 2018 ftunivtroemsoe 2021-06-25T17:55:58Z This is the peer reviewed version of the following article: Ulvestad, J.S., Kumari, K., Seternes, T., Chi, H. & Dalmo, R.A. (2018). Studies on the effects of LPS, ß‐glucan and metabolic inhibitors on the respiratory burst and gene expression in Atlantic salmon macrophages. Journal of Fish Diseases, 41(7), 1117-1127. https://doi.org/10.1111/jfd.12806, which has been published in final form at https://doi.org/10.1111/jfd.12806 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. Reactive oxygen species (ROS) production in macrophage‐like cells is induced as an antimicrobial defence against invading pathogens. In this study, we have explored how different stimuli and metabolic inhibitors affect the level of respiratory burst in Atlantic salmon ( Salmo salar L.) head kidney macrophage‐like cells. Cells stimulated in vitro by bacterial lipopolysaccharide (LPS) and ß‐glucan showed increased production of ROS compared to unstimulated cells. Both stimulation and costimulation by curdlan (ß‐glucan) induced a higher production of ROS compared to stimulation and costimulation by LPS. Metabolic inhibitors co‐incubated with the stimulants did not, in most cases, perturb the level of ROS generation in the salmon macrophage‐like cells. The NAD + content as well as the NAD + /NADH ratio increased in curdlan and LPS + curdlan‐stimulated cells compared to control cells, which indicated increased metabolic activity in the stimulated cells. Supporting these findings, gene analysis using real‐time quantitative PCR showed that arginase‐1 and IL‐1ß genes were highly expressed in the stimulated cells. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Tromsø: Munin Open Research Archive Dalmo ENVELOPE(12.223,12.223,65.137,65.137) Journal of Fish Diseases 41 7 1117 1127 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 gene expression macrophages metabolism respiratory burst salmon |
spellingShingle |
VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 gene expression macrophages metabolism respiratory burst salmon Ulvestad, Johanne Skår Kumari, Jaya Seternes, Tore Chi, Heng Dalmo, Roy Ambli Studies on the effects of LPS, ß‐glucan and metabolic inhibitors on the respiratory burst and gene expression in Atlantic salmon macrophages |
topic_facet |
VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 gene expression macrophages metabolism respiratory burst salmon |
description |
This is the peer reviewed version of the following article: Ulvestad, J.S., Kumari, K., Seternes, T., Chi, H. & Dalmo, R.A. (2018). Studies on the effects of LPS, ß‐glucan and metabolic inhibitors on the respiratory burst and gene expression in Atlantic salmon macrophages. Journal of Fish Diseases, 41(7), 1117-1127. https://doi.org/10.1111/jfd.12806, which has been published in final form at https://doi.org/10.1111/jfd.12806 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. Reactive oxygen species (ROS) production in macrophage‐like cells is induced as an antimicrobial defence against invading pathogens. In this study, we have explored how different stimuli and metabolic inhibitors affect the level of respiratory burst in Atlantic salmon ( Salmo salar L.) head kidney macrophage‐like cells. Cells stimulated in vitro by bacterial lipopolysaccharide (LPS) and ß‐glucan showed increased production of ROS compared to unstimulated cells. Both stimulation and costimulation by curdlan (ß‐glucan) induced a higher production of ROS compared to stimulation and costimulation by LPS. Metabolic inhibitors co‐incubated with the stimulants did not, in most cases, perturb the level of ROS generation in the salmon macrophage‐like cells. The NAD + content as well as the NAD + /NADH ratio increased in curdlan and LPS + curdlan‐stimulated cells compared to control cells, which indicated increased metabolic activity in the stimulated cells. Supporting these findings, gene analysis using real‐time quantitative PCR showed that arginase‐1 and IL‐1ß genes were highly expressed in the stimulated cells. |
format |
Article in Journal/Newspaper |
author |
Ulvestad, Johanne Skår Kumari, Jaya Seternes, Tore Chi, Heng Dalmo, Roy Ambli |
author_facet |
Ulvestad, Johanne Skår Kumari, Jaya Seternes, Tore Chi, Heng Dalmo, Roy Ambli |
author_sort |
Ulvestad, Johanne Skår |
title |
Studies on the effects of LPS, ß‐glucan and metabolic inhibitors on the respiratory burst and gene expression in Atlantic salmon macrophages |
title_short |
Studies on the effects of LPS, ß‐glucan and metabolic inhibitors on the respiratory burst and gene expression in Atlantic salmon macrophages |
title_full |
Studies on the effects of LPS, ß‐glucan and metabolic inhibitors on the respiratory burst and gene expression in Atlantic salmon macrophages |
title_fullStr |
Studies on the effects of LPS, ß‐glucan and metabolic inhibitors on the respiratory burst and gene expression in Atlantic salmon macrophages |
title_full_unstemmed |
Studies on the effects of LPS, ß‐glucan and metabolic inhibitors on the respiratory burst and gene expression in Atlantic salmon macrophages |
title_sort |
studies on the effects of lps, ß‐glucan and metabolic inhibitors on the respiratory burst and gene expression in atlantic salmon macrophages |
publisher |
Wiley |
publishDate |
2018 |
url |
https://hdl.handle.net/10037/13497 |
long_lat |
ENVELOPE(12.223,12.223,65.137,65.137) |
geographic |
Dalmo |
geographic_facet |
Dalmo |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Journal of Fish Diseases info:eu-repo/grantAgreement/RCN/HAVBRUK2/237315/Norway/Fish Virus Vaccines/ViVaFish/ info:eu-repo/grantAgreement/RCN/NANO2021/239140/Norway/Nanoparticle encapsulation of plant-based vaccine against piscine reovirus infection in salmon// Ulvestad, J.S., Kumari, K., Seternes, T., Chi, H. & Dalmo, R.A. (2018). Studies on the effects of LPS, ß‐glucan and metabolic inhibitors on the respiratory burst and gene expression in Atlantic salmon macrophages. Journal of Fish Diseases, 41(7), 1117-1127. https://doi.org/10.1111/jfd.12806 FRIDAID 1576941 0140-7775 1365-2761 https://hdl.handle.net/10037/13497 |
op_rights |
openAccess |
container_title |
Journal of Fish Diseases |
container_volume |
41 |
container_issue |
7 |
container_start_page |
1117 |
op_container_end_page |
1127 |
_version_ |
1766361573506416640 |