Viral assemblage variation in an Arctic shelf seafloor
Accepted manuscript version. Published version available at https://doi.org/10.3354/ame01808 . Spatial differences in microbial communities are observable even in habitats with moderate environmental variation, such as within the pelagic zone or seafloor of geographically finite regions of the ocean...
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Online Access: | https://hdl.handle.net/10037/13125 https://doi.org/10.3354/ame01808 |
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ftunivtroemsoe:oai:munin.uit.no:10037/13125 2023-05-15T14:24:53+02:00 Viral assemblage variation in an Arctic shelf seafloor Nguyen, Tan Thi Robertsen, Espen Mikal Landfald, Bjarne 2017-02-09 https://hdl.handle.net/10037/13125 https://doi.org/10.3354/ame01808 eng eng Inter Research Aquatic Microbial Ecology Nguyen, T.T., Robertsen, E.M. & Landfald, B. (2017). Viral assemblage variation in an Arctic shelf seafloor. Aquatic Microbial Ecology, 78(3), 135-145. https://doi.org/10.3354/ame01808 FRIDAID 1443825 doi:10.3354/ame01808 0948-3055 1616-1564 https://hdl.handle.net/10037/13125 openAccess VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 Viruses ssDNA virus Barents Sea Marine sediments Biogeography Journal article Tidsskriftartikkel Peer reviewed 2017 ftunivtroemsoe https://doi.org/10.3354/ame01808 2021-06-25T17:55:46Z Accepted manuscript version. Published version available at https://doi.org/10.3354/ame01808 . Spatial differences in microbial communities are observable even in habitats with moderate environmental variation, such as within the pelagic zone or seafloor of geographically finite regions of the oceans. Here we explore whether biogeographical variations are also manifested at this level in the structure of viral assemblages by comparing DNA viromes from the Barents Sea upper seafloor, collected at 5 geographically separated locations. Of the open reading frames, 27 to 44% showed significant similarity to genes of viral genomes in the Refseq database. The majority of the identified open reading frames, i.e. 86 to 95%, were affiliated with sequences of single-stranded DNA (ssDNA) viruses, but the ssDNA virus genetic material was likely strongly overrepresented due to the use of phi29 DNA polymerase for amplifying viral DNA. The majority of ssDNA virus sequences originated from the Microviridae family of phages and the eukaryotic Circular Rep-encoding ssDNA (CRESS-DNA) viruses. The sediment virus assemblages showed higher overall similarity to counterparts from deep-sea sediment of the Pacific Ocean than to, e.g., Arctic Ocean pelagic viromes, supporting the presence of common compositional features in sediment viral assemblages across continental-scale geographical separations. The Barents Sea viromes grouped biogeographically in accordance with the south-north environmental division of this Arctic sea by the oceanic polar front, thereby mirroring a corresponding 16S rRNA gene-based biogeographical division of the bacterial communities. However, compositional differences in the eukaryotic virus assemblages rather than the bacteriophages appeared to be the primary basis for this spatial separation. Article in Journal/Newspaper Arctic Arctic Arctic Ocean Barents Sea University of Tromsø: Munin Open Research Archive Arctic Arctic Ocean Barents Sea Pacific Aquatic Microbial Ecology 78 3 135 145 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 Viruses ssDNA virus Barents Sea Marine sediments Biogeography |
spellingShingle |
VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 Viruses ssDNA virus Barents Sea Marine sediments Biogeography Nguyen, Tan Thi Robertsen, Espen Mikal Landfald, Bjarne Viral assemblage variation in an Arctic shelf seafloor |
topic_facet |
VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 Viruses ssDNA virus Barents Sea Marine sediments Biogeography |
description |
Accepted manuscript version. Published version available at https://doi.org/10.3354/ame01808 . Spatial differences in microbial communities are observable even in habitats with moderate environmental variation, such as within the pelagic zone or seafloor of geographically finite regions of the oceans. Here we explore whether biogeographical variations are also manifested at this level in the structure of viral assemblages by comparing DNA viromes from the Barents Sea upper seafloor, collected at 5 geographically separated locations. Of the open reading frames, 27 to 44% showed significant similarity to genes of viral genomes in the Refseq database. The majority of the identified open reading frames, i.e. 86 to 95%, were affiliated with sequences of single-stranded DNA (ssDNA) viruses, but the ssDNA virus genetic material was likely strongly overrepresented due to the use of phi29 DNA polymerase for amplifying viral DNA. The majority of ssDNA virus sequences originated from the Microviridae family of phages and the eukaryotic Circular Rep-encoding ssDNA (CRESS-DNA) viruses. The sediment virus assemblages showed higher overall similarity to counterparts from deep-sea sediment of the Pacific Ocean than to, e.g., Arctic Ocean pelagic viromes, supporting the presence of common compositional features in sediment viral assemblages across continental-scale geographical separations. The Barents Sea viromes grouped biogeographically in accordance with the south-north environmental division of this Arctic sea by the oceanic polar front, thereby mirroring a corresponding 16S rRNA gene-based biogeographical division of the bacterial communities. However, compositional differences in the eukaryotic virus assemblages rather than the bacteriophages appeared to be the primary basis for this spatial separation. |
format |
Article in Journal/Newspaper |
author |
Nguyen, Tan Thi Robertsen, Espen Mikal Landfald, Bjarne |
author_facet |
Nguyen, Tan Thi Robertsen, Espen Mikal Landfald, Bjarne |
author_sort |
Nguyen, Tan Thi |
title |
Viral assemblage variation in an Arctic shelf seafloor |
title_short |
Viral assemblage variation in an Arctic shelf seafloor |
title_full |
Viral assemblage variation in an Arctic shelf seafloor |
title_fullStr |
Viral assemblage variation in an Arctic shelf seafloor |
title_full_unstemmed |
Viral assemblage variation in an Arctic shelf seafloor |
title_sort |
viral assemblage variation in an arctic shelf seafloor |
publisher |
Inter Research |
publishDate |
2017 |
url |
https://hdl.handle.net/10037/13125 https://doi.org/10.3354/ame01808 |
geographic |
Arctic Arctic Ocean Barents Sea Pacific |
geographic_facet |
Arctic Arctic Ocean Barents Sea Pacific |
genre |
Arctic Arctic Arctic Ocean Barents Sea |
genre_facet |
Arctic Arctic Arctic Ocean Barents Sea |
op_relation |
Aquatic Microbial Ecology Nguyen, T.T., Robertsen, E.M. & Landfald, B. (2017). Viral assemblage variation in an Arctic shelf seafloor. Aquatic Microbial Ecology, 78(3), 135-145. https://doi.org/10.3354/ame01808 FRIDAID 1443825 doi:10.3354/ame01808 0948-3055 1616-1564 https://hdl.handle.net/10037/13125 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.3354/ame01808 |
container_title |
Aquatic Microbial Ecology |
container_volume |
78 |
container_issue |
3 |
container_start_page |
135 |
op_container_end_page |
145 |
_version_ |
1766297328257335296 |