Molecular transfer to Atlantic salmon ovulated eggs using liposomes
Published version available in Aquaculture. 2017;479:404-411. The delivery of exogenous biomolecules into teleost eggs is currently mostly relying on the manual microinjection methods, which, due to their high costs and low throughput, are not economically feasible for large-scale aquaculture applic...
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Online Access: | https://hdl.handle.net/10037/12445 https://doi.org/10.1016/j.aquaculture.2017.06.019 |
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ftunivtroemsoe:oai:munin.uit.no:10037/12445 2023-05-15T15:30:08+02:00 Molecular transfer to Atlantic salmon ovulated eggs using liposomes Kumari, Jaya Flaten, Gøril Eide Skalko-Basnet, Natasa Tveiten, Helge 2017-06-15 https://hdl.handle.net/10037/12445 https://doi.org/10.1016/j.aquaculture.2017.06.019 eng eng Elsevier Aquaculture info:eu-repo/grantAgreement/RCN/BIOTEK2021/221648/NORWAY/Sterile salmon by targeting factors involved in germ cell survival: novel vaccination strategies for sustainable fish farming/Sketch 40/ Kumari, J., Flaten, G. E., Škalko-Basnet, N., Tveiten, H. (2017). Molecular transfer to Atlantic salmon ovulated eggs using liposomes. Aquaculture, 479:404-411. FRIDAID 1476627 doi:10.1016/j.aquaculture.2017.06.019 0044-8486 1873-5622 https://hdl.handle.net/10037/12445 openAccess VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Akvakultur: 922 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922 Delivery Liposomes Rhodamine B Rhodamine B isothiocyanate Egg Atlantic salmon Journal article Tidsskriftartikkel Peer reviewed 2017 ftunivtroemsoe https://doi.org/10.1016/j.aquaculture.2017.06.019 2021-06-25T17:55:30Z Published version available in Aquaculture. 2017;479:404-411. The delivery of exogenous biomolecules into teleost eggs is currently mostly relying on the manual microinjection methods, which, due to their high costs and low throughput, are not economically feasible for large-scale aquaculture applications. The main objective of this study was to develop a convenient, simple immersion delivery model for unfertilized Atlantic salmon (Salmo salar L.) eggs using liposomes as a system for the delivery of selected molecules. We used a lipophilic marker, Rhodamine B isothiocyanate (RBITC) dye, to follow liposomal uptake, marker release and marker distribution within incubated egg. We investigated the influence of the incubation time, liposome surface charges and liposome sizes on the uptake into the eggs. After incubation with liposomes, the exposed eggs were fertilized and embryonic development was monitored until complete yolk sac absorption. The result showed that RBITC, was successfully delivered to the yolk of the incubated eggs. Moreover, during embryonic development, liposomal RBITC remained in the yolk sac until the yolk was completely absorbed. The findings demonstrated a novel approach for the delivery of exogenous molecules to unfertilized Atlantic salmon eggs, opening an avenue for large-scale aquaculture therapeutic applications. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Tromsø: Munin Open Research Archive Aquaculture 479 404 411 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Akvakultur: 922 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922 Delivery Liposomes Rhodamine B Rhodamine B isothiocyanate Egg Atlantic salmon |
spellingShingle |
VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Akvakultur: 922 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922 Delivery Liposomes Rhodamine B Rhodamine B isothiocyanate Egg Atlantic salmon Kumari, Jaya Flaten, Gøril Eide Skalko-Basnet, Natasa Tveiten, Helge Molecular transfer to Atlantic salmon ovulated eggs using liposomes |
topic_facet |
VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Akvakultur: 922 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922 Delivery Liposomes Rhodamine B Rhodamine B isothiocyanate Egg Atlantic salmon |
description |
Published version available in Aquaculture. 2017;479:404-411. The delivery of exogenous biomolecules into teleost eggs is currently mostly relying on the manual microinjection methods, which, due to their high costs and low throughput, are not economically feasible for large-scale aquaculture applications. The main objective of this study was to develop a convenient, simple immersion delivery model for unfertilized Atlantic salmon (Salmo salar L.) eggs using liposomes as a system for the delivery of selected molecules. We used a lipophilic marker, Rhodamine B isothiocyanate (RBITC) dye, to follow liposomal uptake, marker release and marker distribution within incubated egg. We investigated the influence of the incubation time, liposome surface charges and liposome sizes on the uptake into the eggs. After incubation with liposomes, the exposed eggs were fertilized and embryonic development was monitored until complete yolk sac absorption. The result showed that RBITC, was successfully delivered to the yolk of the incubated eggs. Moreover, during embryonic development, liposomal RBITC remained in the yolk sac until the yolk was completely absorbed. The findings demonstrated a novel approach for the delivery of exogenous molecules to unfertilized Atlantic salmon eggs, opening an avenue for large-scale aquaculture therapeutic applications. |
format |
Article in Journal/Newspaper |
author |
Kumari, Jaya Flaten, Gøril Eide Skalko-Basnet, Natasa Tveiten, Helge |
author_facet |
Kumari, Jaya Flaten, Gøril Eide Skalko-Basnet, Natasa Tveiten, Helge |
author_sort |
Kumari, Jaya |
title |
Molecular transfer to Atlantic salmon ovulated eggs using liposomes |
title_short |
Molecular transfer to Atlantic salmon ovulated eggs using liposomes |
title_full |
Molecular transfer to Atlantic salmon ovulated eggs using liposomes |
title_fullStr |
Molecular transfer to Atlantic salmon ovulated eggs using liposomes |
title_full_unstemmed |
Molecular transfer to Atlantic salmon ovulated eggs using liposomes |
title_sort |
molecular transfer to atlantic salmon ovulated eggs using liposomes |
publisher |
Elsevier |
publishDate |
2017 |
url |
https://hdl.handle.net/10037/12445 https://doi.org/10.1016/j.aquaculture.2017.06.019 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Aquaculture info:eu-repo/grantAgreement/RCN/BIOTEK2021/221648/NORWAY/Sterile salmon by targeting factors involved in germ cell survival: novel vaccination strategies for sustainable fish farming/Sketch 40/ Kumari, J., Flaten, G. E., Škalko-Basnet, N., Tveiten, H. (2017). Molecular transfer to Atlantic salmon ovulated eggs using liposomes. Aquaculture, 479:404-411. FRIDAID 1476627 doi:10.1016/j.aquaculture.2017.06.019 0044-8486 1873-5622 https://hdl.handle.net/10037/12445 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1016/j.aquaculture.2017.06.019 |
container_title |
Aquaculture |
container_volume |
479 |
container_start_page |
404 |
op_container_end_page |
411 |
_version_ |
1766360580591976448 |