Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers
Background: Colonization of the body is an important step in Staphylococcus aureus infection. S. aureus colonizes skin and mucous membranes in humans and several animal species. One important ecological niche of S. aureus is the anterior nares. More than 60% of the S. aureus in the nose are found in...
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ftunivtroemsoe:oai:munin.uit.no:10037/11278 2023-05-15T18:34:46+02:00 Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers Hanssen, Anne-Merethe Kindlund, Bert Stenklev, Niels Christian Furberg, Anne-Sofie Fismen, Silje Olsen, Renate Slind Johannessen, Mona Sollid, Johanna U Ericson 2017-04-05 https://hdl.handle.net/10037/11278 https://doi.org/10.1186/s12866-017-0997-3 eng eng BioMed Central BMC Microbiology Hanssen AM, Kindlund B, Stenklev NC, Furberg AS, Fismen S, Olsen RS, Johannessen M, Sollid jue. Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers. BMC Microbiology. 2017;17(89) FRIDAID 1464601 doi:10.1186/s12866-017-0997-3 1471-2180 https://hdl.handle.net/10037/11278 openAccess Nasal colonization Staphylococcus aureus Carrier Localization Epithelial cells VDP::Medisinske Fag: 700::Basale medisinske odontologiske og veterinærmedisinske fag: 710::Medisinsk mikrobiologi: 715 VDP::Medical disciplines: 700::Basic medical dental and veterinary science disciplines: 710::Medical microbiology: 715 Journal article Tidsskriftartikkel Peer reviewed 2017 ftunivtroemsoe https://doi.org/10.1186/s12866-017-0997-3 2021-06-25T17:55:13Z Background: Colonization of the body is an important step in Staphylococcus aureus infection. S. aureus colonizes skin and mucous membranes in humans and several animal species. One important ecological niche of S. aureus is the anterior nares. More than 60% of the S. aureus in the nose are found in vestibulum nasi. Our aim was to describe the localization of S. aureus in nasal tissue from healthy carriers. Methods: Punch skin biopsies were taken from vestibulum nasi from healthy volunteers (S. aureus carriers and non−/intermittent carriers, n = 39) attending the population-based Tromsø 6 study. The tissue samples were processed as frozen sections before immunostaining with a specific S. aureus antibody, and finally evaluated by a confocal laser-scanning microscope. Results: Our results suggest that S. aureus colonize both the upper and lower layers of the epidermis within the nasal epithelium of healthy individuals. The number of S. aureus in epidermis was surprisingly low. Intracellular localization of S. aureus in nasal tissue from healthy individuals was also detected. Conclusions: Knowledge of the exact localization of S. aureus in nasal tissue is important for the understanding of the host responses against S. aureus. Our results may have consequences for the eradication strategy of S. aureus in carriers, and further work can provide us with tools for targeted prevention of S. aureus colonisation and infection. Article in Journal/Newspaper Tromsø University of Tromsø: Munin Open Research Archive Nares ENVELOPE(158.167,158.167,-81.450,-81.450) Nasi ENVELOPE(-23.036,-23.036,66.336,66.336) Tromsø BMC Microbiology 17 1 |
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Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
Nasal colonization Staphylococcus aureus Carrier Localization Epithelial cells VDP::Medisinske Fag: 700::Basale medisinske odontologiske og veterinærmedisinske fag: 710::Medisinsk mikrobiologi: 715 VDP::Medical disciplines: 700::Basic medical dental and veterinary science disciplines: 710::Medical microbiology: 715 |
spellingShingle |
Nasal colonization Staphylococcus aureus Carrier Localization Epithelial cells VDP::Medisinske Fag: 700::Basale medisinske odontologiske og veterinærmedisinske fag: 710::Medisinsk mikrobiologi: 715 VDP::Medical disciplines: 700::Basic medical dental and veterinary science disciplines: 710::Medical microbiology: 715 Hanssen, Anne-Merethe Kindlund, Bert Stenklev, Niels Christian Furberg, Anne-Sofie Fismen, Silje Olsen, Renate Slind Johannessen, Mona Sollid, Johanna U Ericson Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers |
topic_facet |
Nasal colonization Staphylococcus aureus Carrier Localization Epithelial cells VDP::Medisinske Fag: 700::Basale medisinske odontologiske og veterinærmedisinske fag: 710::Medisinsk mikrobiologi: 715 VDP::Medical disciplines: 700::Basic medical dental and veterinary science disciplines: 710::Medical microbiology: 715 |
description |
Background: Colonization of the body is an important step in Staphylococcus aureus infection. S. aureus colonizes skin and mucous membranes in humans and several animal species. One important ecological niche of S. aureus is the anterior nares. More than 60% of the S. aureus in the nose are found in vestibulum nasi. Our aim was to describe the localization of S. aureus in nasal tissue from healthy carriers. Methods: Punch skin biopsies were taken from vestibulum nasi from healthy volunteers (S. aureus carriers and non−/intermittent carriers, n = 39) attending the population-based Tromsø 6 study. The tissue samples were processed as frozen sections before immunostaining with a specific S. aureus antibody, and finally evaluated by a confocal laser-scanning microscope. Results: Our results suggest that S. aureus colonize both the upper and lower layers of the epidermis within the nasal epithelium of healthy individuals. The number of S. aureus in epidermis was surprisingly low. Intracellular localization of S. aureus in nasal tissue from healthy individuals was also detected. Conclusions: Knowledge of the exact localization of S. aureus in nasal tissue is important for the understanding of the host responses against S. aureus. Our results may have consequences for the eradication strategy of S. aureus in carriers, and further work can provide us with tools for targeted prevention of S. aureus colonisation and infection. |
format |
Article in Journal/Newspaper |
author |
Hanssen, Anne-Merethe Kindlund, Bert Stenklev, Niels Christian Furberg, Anne-Sofie Fismen, Silje Olsen, Renate Slind Johannessen, Mona Sollid, Johanna U Ericson |
author_facet |
Hanssen, Anne-Merethe Kindlund, Bert Stenklev, Niels Christian Furberg, Anne-Sofie Fismen, Silje Olsen, Renate Slind Johannessen, Mona Sollid, Johanna U Ericson |
author_sort |
Hanssen, Anne-Merethe |
title |
Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers |
title_short |
Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers |
title_full |
Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers |
title_fullStr |
Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers |
title_full_unstemmed |
Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers |
title_sort |
localization of staphylococcus aureus in tissue from the nasal vestibule in healthy carriers |
publisher |
BioMed Central |
publishDate |
2017 |
url |
https://hdl.handle.net/10037/11278 https://doi.org/10.1186/s12866-017-0997-3 |
long_lat |
ENVELOPE(158.167,158.167,-81.450,-81.450) ENVELOPE(-23.036,-23.036,66.336,66.336) |
geographic |
Nares Nasi Tromsø |
geographic_facet |
Nares Nasi Tromsø |
genre |
Tromsø |
genre_facet |
Tromsø |
op_relation |
BMC Microbiology Hanssen AM, Kindlund B, Stenklev NC, Furberg AS, Fismen S, Olsen RS, Johannessen M, Sollid jue. Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers. BMC Microbiology. 2017;17(89) FRIDAID 1464601 doi:10.1186/s12866-017-0997-3 1471-2180 https://hdl.handle.net/10037/11278 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1186/s12866-017-0997-3 |
container_title |
BMC Microbiology |
container_volume |
17 |
container_issue |
1 |
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1766219671760011264 |