Pan genome and CRISPR analyses of the bacterial fish pathogen Moritella viscosa
Source at http://dx.doi.org/10.1186/s12864-017-3693-7 Background: Winter-ulcer Moritella viscosa infections continue to be a significant burden in Atlantic salmon (Salmo salar L.) farming. M. viscosa comprises two main clusters that differ in genetic variation and phenotypes including virulence. Hor...
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ftunivtroemsoe:oai:munin.uit.no:10037/11125 2023-05-15T15:32:42+02:00 Pan genome and CRISPR analyses of the bacterial fish pathogen Moritella viscosa Karlsen, Christian Hjerde, Erik Klemetsen, Terje Willassen, Nils Peder 2017-04-20 https://hdl.handle.net/10037/11125 https://doi.org/10.1186/s12864-017-3693-7 eng eng BioMed Central BMC Genomics Norges forskningsråd: 216196 Nofima AS: 11268 eu-repo/grantAgreement/RCN/HAVBRUK/216196/NORWAY/Coordinated Bacterial Virulence: Relevance in Winter Ulcer// Karlsen C, Hjerde E, Klemetsen T, Willassen NP. Pan genome and CRISPR analyses of the bacterial fish pathogen Moritella viscosa. BMC Genomics. 2017;18:313 FRIDAID 1469050 doi:10.1186/s12864-017-3693-7 1471-2164 https://hdl.handle.net/10037/11125 openAccess VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 Journal article Tidsskriftartikkel Peer reviewed 2017 ftunivtroemsoe https://doi.org/10.1186/s12864-017-3693-7 2021-06-25T17:55:17Z Source at http://dx.doi.org/10.1186/s12864-017-3693-7 Background: Winter-ulcer Moritella viscosa infections continue to be a significant burden in Atlantic salmon (Salmo salar L.) farming. M. viscosa comprises two main clusters that differ in genetic variation and phenotypes including virulence. Horizontal gene transfer through acquisition and loss of mobile genetic elements (MGEs) is a major driving force of bacterial diversification. To gain insight into genomic traits that could affect sublineage evolution within this bacterium we examined the genome sequences of twelve M. viscosa strains. Matches between M. viscosa clustered, regularly interspaced, short palindromic, repeats and associated cas genes (CRISPR-Cas) were analysed to correlate CRISPR-Cas with adaptive immunity against MGEs. Results: The comparative genomic analysis of M. viscosa isolates from across the North Atlantic region and from different fish species support delineation of M. viscosa into four phylogenetic lineages. The results showed that M. viscosa carries two distinct variants of the CRISPR-Cas subtype I-F systems and that CRISPR features follow the phylogenetic lineages. A subset of the spacer content match prophage and plasmid genes dispersed among the M. viscosa strains. Further analysis revealed that prophage and plasmid-like element distribution were reflected in the content of the CRISPR-spacer profiles. Conclusions: Our data suggests that CRISPR-Cas mediated interactions with MGEs impact genome properties among M. viscosa, and that patterns in spacer and MGE distributions are linked to strain relationships. Article in Journal/Newspaper Atlantic salmon North Atlantic Salmo salar University of Tromsø: Munin Open Research Archive BMC Genomics 18 1 |
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University of Tromsø: Munin Open Research Archive |
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ftunivtroemsoe |
language |
English |
topic |
VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 |
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VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 Karlsen, Christian Hjerde, Erik Klemetsen, Terje Willassen, Nils Peder Pan genome and CRISPR analyses of the bacterial fish pathogen Moritella viscosa |
topic_facet |
VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 |
description |
Source at http://dx.doi.org/10.1186/s12864-017-3693-7 Background: Winter-ulcer Moritella viscosa infections continue to be a significant burden in Atlantic salmon (Salmo salar L.) farming. M. viscosa comprises two main clusters that differ in genetic variation and phenotypes including virulence. Horizontal gene transfer through acquisition and loss of mobile genetic elements (MGEs) is a major driving force of bacterial diversification. To gain insight into genomic traits that could affect sublineage evolution within this bacterium we examined the genome sequences of twelve M. viscosa strains. Matches between M. viscosa clustered, regularly interspaced, short palindromic, repeats and associated cas genes (CRISPR-Cas) were analysed to correlate CRISPR-Cas with adaptive immunity against MGEs. Results: The comparative genomic analysis of M. viscosa isolates from across the North Atlantic region and from different fish species support delineation of M. viscosa into four phylogenetic lineages. The results showed that M. viscosa carries two distinct variants of the CRISPR-Cas subtype I-F systems and that CRISPR features follow the phylogenetic lineages. A subset of the spacer content match prophage and plasmid genes dispersed among the M. viscosa strains. Further analysis revealed that prophage and plasmid-like element distribution were reflected in the content of the CRISPR-spacer profiles. Conclusions: Our data suggests that CRISPR-Cas mediated interactions with MGEs impact genome properties among M. viscosa, and that patterns in spacer and MGE distributions are linked to strain relationships. |
format |
Article in Journal/Newspaper |
author |
Karlsen, Christian Hjerde, Erik Klemetsen, Terje Willassen, Nils Peder |
author_facet |
Karlsen, Christian Hjerde, Erik Klemetsen, Terje Willassen, Nils Peder |
author_sort |
Karlsen, Christian |
title |
Pan genome and CRISPR analyses of the bacterial fish pathogen Moritella viscosa |
title_short |
Pan genome and CRISPR analyses of the bacterial fish pathogen Moritella viscosa |
title_full |
Pan genome and CRISPR analyses of the bacterial fish pathogen Moritella viscosa |
title_fullStr |
Pan genome and CRISPR analyses of the bacterial fish pathogen Moritella viscosa |
title_full_unstemmed |
Pan genome and CRISPR analyses of the bacterial fish pathogen Moritella viscosa |
title_sort |
pan genome and crispr analyses of the bacterial fish pathogen moritella viscosa |
publisher |
BioMed Central |
publishDate |
2017 |
url |
https://hdl.handle.net/10037/11125 https://doi.org/10.1186/s12864-017-3693-7 |
genre |
Atlantic salmon North Atlantic Salmo salar |
genre_facet |
Atlantic salmon North Atlantic Salmo salar |
op_relation |
BMC Genomics Norges forskningsråd: 216196 Nofima AS: 11268 eu-repo/grantAgreement/RCN/HAVBRUK/216196/NORWAY/Coordinated Bacterial Virulence: Relevance in Winter Ulcer// Karlsen C, Hjerde E, Klemetsen T, Willassen NP. Pan genome and CRISPR analyses of the bacterial fish pathogen Moritella viscosa. BMC Genomics. 2017;18:313 FRIDAID 1469050 doi:10.1186/s12864-017-3693-7 1471-2164 https://hdl.handle.net/10037/11125 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1186/s12864-017-3693-7 |
container_title |
BMC Genomics |
container_volume |
18 |
container_issue |
1 |
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1766363196461940736 |