Experimental challenge of atlantic cod (Gadus morhua) with a brucella pinnipedialis strain from hooded seal (Cystophora cristata)
Link to publishers version: 10.1371/journal.pone.0159272 Pathology has not been observed in true seals infected with Brucella pinnipedialis. A lack of intracellular survival and multiplication of B. pinnipedialis in hooded seal (Cystophora cristata) macrophages in vitro indicates a lack of chronic i...
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ftunivtroemsoe:oai:munin.uit.no:10037/10623 2023-05-15T15:26:54+02:00 Experimental challenge of atlantic cod (Gadus morhua) with a brucella pinnipedialis strain from hooded seal (Cystophora cristata) Nymo, Ingebjørg Helena Seppola, Marit Sascha, Al Dahouk Bakkemo, Katrine Ryvold Arnesen Jiménez de Bagüés, María Pilar Godfroid, Jacques Larsen, Anett Kristin 2016-07-14 https://hdl.handle.net/10037/10623 https://doi.org/10.1371/journal.pone.0159272 eng eng PLOS PLoS ONE Nymo IH, Seppola M, Sascha, Bakkemo KRA, Jiménez de Bagüés MP, Godfroid J, Larsen AK. Experimental challenge of atlantic cod (Gadus morhua) with a brucella pinnipedialis strain from hooded seal (Cystophora cristata). PLoS ONE. 2016;11(7) FRIDAID 1370177 doi:10.1371/journal.pone.0159272 1932-6203 https://hdl.handle.net/10037/10623 openAccess VDP::Agriculture and fishery disciplines: 900 Peer reviewed 2016 ftunivtroemsoe https://doi.org/10.1371/journal.pone.0159272 2021-06-25T17:55:03Z Link to publishers version: 10.1371/journal.pone.0159272 Pathology has not been observed in true seals infected with Brucella pinnipedialis. A lack of intracellular survival and multiplication of B. pinnipedialis in hooded seal (Cystophora cristata) macrophages in vitro indicates a lack of chronic infection in hooded seals. Both epidemiology and bacteriological patterns in the hooded seal point to a transient infection of environmental origin, possibly through the food chain. To analyse the potential role of fish in the transmission of B. pinnipedialis, Atlantic cod (Gadus morhua) were injected intraperitoneally with 7.5 x 107 bacteria of a hooded seal field isolate. Samples of blood, liver, spleen, muscle, heart, head kidney, female gonads and feces were collected on days 1, 7, 14 and 28 post infection to assess the bacterial load, and to determine the expression of immune genes and the specific antibody response. Challenged fish showed an extended period of bacteremia through day 14 and viable bacteria were observed in all organs sampled, except muscle, until day 28. Neither gross lesions nor mortality were recorded. Anti-Brucella antibodies were detected from day 14 onwards and the expression of hepcidin, cathelicidin, interleukin (IL)-1β, IL-10, and interferon (IFN)-γ genes were significantly increased in spleen at day 1 and 28. Primary mononuclear cells isolated from head kidneys of Atlantic cod were exposed to B. pinnipedialis reference (NCTC 12890) and hooded seal (17a-1) strain. Both bacterial strains invaded mononuclear cells and survived intracellularly without any major reduction in bacterial counts for at least 48 hours. Our study shows that the B. pinnipedialis strain isolated from hooded seal survives in Atlantic cod, and suggests that Atlantic cod could play a role in the transmission of B. pinnipedialis to hooded seals in the wild. Text atlantic cod Cystophora cristata Gadus morhua hooded seal University of Tromsø: Munin Open Research Archive Seal Point ENVELOPE(3.319,3.319,-54.451,-54.451) PLOS ONE 11 7 e0159272 |
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Open Polar |
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University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Agriculture and fishery disciplines: 900 |
spellingShingle |
VDP::Agriculture and fishery disciplines: 900 Nymo, Ingebjørg Helena Seppola, Marit Sascha, Al Dahouk Bakkemo, Katrine Ryvold Arnesen Jiménez de Bagüés, María Pilar Godfroid, Jacques Larsen, Anett Kristin Experimental challenge of atlantic cod (Gadus morhua) with a brucella pinnipedialis strain from hooded seal (Cystophora cristata) |
topic_facet |
VDP::Agriculture and fishery disciplines: 900 |
description |
Link to publishers version: 10.1371/journal.pone.0159272 Pathology has not been observed in true seals infected with Brucella pinnipedialis. A lack of intracellular survival and multiplication of B. pinnipedialis in hooded seal (Cystophora cristata) macrophages in vitro indicates a lack of chronic infection in hooded seals. Both epidemiology and bacteriological patterns in the hooded seal point to a transient infection of environmental origin, possibly through the food chain. To analyse the potential role of fish in the transmission of B. pinnipedialis, Atlantic cod (Gadus morhua) were injected intraperitoneally with 7.5 x 107 bacteria of a hooded seal field isolate. Samples of blood, liver, spleen, muscle, heart, head kidney, female gonads and feces were collected on days 1, 7, 14 and 28 post infection to assess the bacterial load, and to determine the expression of immune genes and the specific antibody response. Challenged fish showed an extended period of bacteremia through day 14 and viable bacteria were observed in all organs sampled, except muscle, until day 28. Neither gross lesions nor mortality were recorded. Anti-Brucella antibodies were detected from day 14 onwards and the expression of hepcidin, cathelicidin, interleukin (IL)-1β, IL-10, and interferon (IFN)-γ genes were significantly increased in spleen at day 1 and 28. Primary mononuclear cells isolated from head kidneys of Atlantic cod were exposed to B. pinnipedialis reference (NCTC 12890) and hooded seal (17a-1) strain. Both bacterial strains invaded mononuclear cells and survived intracellularly without any major reduction in bacterial counts for at least 48 hours. Our study shows that the B. pinnipedialis strain isolated from hooded seal survives in Atlantic cod, and suggests that Atlantic cod could play a role in the transmission of B. pinnipedialis to hooded seals in the wild. |
format |
Text |
author |
Nymo, Ingebjørg Helena Seppola, Marit Sascha, Al Dahouk Bakkemo, Katrine Ryvold Arnesen Jiménez de Bagüés, María Pilar Godfroid, Jacques Larsen, Anett Kristin |
author_facet |
Nymo, Ingebjørg Helena Seppola, Marit Sascha, Al Dahouk Bakkemo, Katrine Ryvold Arnesen Jiménez de Bagüés, María Pilar Godfroid, Jacques Larsen, Anett Kristin |
author_sort |
Nymo, Ingebjørg Helena |
title |
Experimental challenge of atlantic cod (Gadus morhua) with a brucella pinnipedialis strain from hooded seal (Cystophora cristata) |
title_short |
Experimental challenge of atlantic cod (Gadus morhua) with a brucella pinnipedialis strain from hooded seal (Cystophora cristata) |
title_full |
Experimental challenge of atlantic cod (Gadus morhua) with a brucella pinnipedialis strain from hooded seal (Cystophora cristata) |
title_fullStr |
Experimental challenge of atlantic cod (Gadus morhua) with a brucella pinnipedialis strain from hooded seal (Cystophora cristata) |
title_full_unstemmed |
Experimental challenge of atlantic cod (Gadus morhua) with a brucella pinnipedialis strain from hooded seal (Cystophora cristata) |
title_sort |
experimental challenge of atlantic cod (gadus morhua) with a brucella pinnipedialis strain from hooded seal (cystophora cristata) |
publisher |
PLOS |
publishDate |
2016 |
url |
https://hdl.handle.net/10037/10623 https://doi.org/10.1371/journal.pone.0159272 |
long_lat |
ENVELOPE(3.319,3.319,-54.451,-54.451) |
geographic |
Seal Point |
geographic_facet |
Seal Point |
genre |
atlantic cod Cystophora cristata Gadus morhua hooded seal |
genre_facet |
atlantic cod Cystophora cristata Gadus morhua hooded seal |
op_relation |
PLoS ONE Nymo IH, Seppola M, Sascha, Bakkemo KRA, Jiménez de Bagüés MP, Godfroid J, Larsen AK. Experimental challenge of atlantic cod (Gadus morhua) with a brucella pinnipedialis strain from hooded seal (Cystophora cristata). PLoS ONE. 2016;11(7) FRIDAID 1370177 doi:10.1371/journal.pone.0159272 1932-6203 https://hdl.handle.net/10037/10623 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0159272 |
container_title |
PLOS ONE |
container_volume |
11 |
container_issue |
7 |
container_start_page |
e0159272 |
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1766357379778084864 |