Identification of the Ipomoea nil cDNA clone encoding protein with one transmembrane domain by differential display PCR
A modified differential display procedure was used to compare Ipomoea nil gene expression under flower inductive and non-inductive light/dark conditions and a 170 bp cDNA fragment was displayed. Screening of the cDNA library from I. nil cotyledons led to the isolation of a 577 bp cDNA clone with hig...
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Uniwersytet Mikołaja Kopernika w Toruniu
2020
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ftunivtorunojs:oai:apcz.umk.pl:article/29800 2023-05-15T14:15:25+02:00 Identification of the Ipomoea nil cDNA clone encoding protein with one transmembrane domain by differential display PCR Dąbrowska, Grażyna Barbara Dąbrowski, Henryk Paweł Richert, Agnieszka 2020-04-21 application/pdf https://apcz.umk.pl/EQ/article/view/EQ.2020.015 eng eng Uniwersytet Mikołaja Kopernika w Toruniu https://apcz.umk.pl/EQ/article/view/EQ.2020.015/25395 https://apcz.umk.pl/EQ/article/view/EQ.2020.015 Prawa autorskie (c) 2020 Ecological Questions Ecological Questions; Vol. 31 No. 2 (2020); 71-78 Ecological Questions; Tom 31 Nr 2 (2020); 71-78 1644-7298 chlorophyll a/b binding protein (CAB) One Helix Protein (OHP) Pharbitis nil flower induction light stress differential display PCR info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2020 ftunivtorunojs 2022-08-19T07:21:01Z A modified differential display procedure was used to compare Ipomoea nil gene expression under flower inductive and non-inductive light/dark conditions and a 170 bp cDNA fragment was displayed. Screening of the cDNA library from I. nil cotyledons led to the isolation of a 577 bp cDNA clone with high nucleotide and amino acids homology to One Helix Protein (OHP) genes from Arabidopsis thaliana and Deschampsia antartica. The membrane spanning helix of InOHP is 91% identical to the MSH of the A. thaliana OHP. MSH is located in the C-terminal part of InOHP protein and corresponds to third MSH of LHC proteins. InOHP transcripts are numerous in leaves of plants when grown under continuous light and are also present in grown under flower inductive condition but their quantity is lower. Southern analysis showed that InOHP is member of a gene family involved in photoprotection of photosystems against excessive light Article in Journal/Newspaper antartic* Akademicka Platforma Czasopism (APCZ) |
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Open Polar |
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Akademicka Platforma Czasopism (APCZ) |
op_collection_id |
ftunivtorunojs |
language |
English |
topic |
chlorophyll a/b binding protein (CAB) One Helix Protein (OHP) Pharbitis nil flower induction light stress differential display PCR |
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chlorophyll a/b binding protein (CAB) One Helix Protein (OHP) Pharbitis nil flower induction light stress differential display PCR Dąbrowska, Grażyna Barbara Dąbrowski, Henryk Paweł Richert, Agnieszka Identification of the Ipomoea nil cDNA clone encoding protein with one transmembrane domain by differential display PCR |
topic_facet |
chlorophyll a/b binding protein (CAB) One Helix Protein (OHP) Pharbitis nil flower induction light stress differential display PCR |
description |
A modified differential display procedure was used to compare Ipomoea nil gene expression under flower inductive and non-inductive light/dark conditions and a 170 bp cDNA fragment was displayed. Screening of the cDNA library from I. nil cotyledons led to the isolation of a 577 bp cDNA clone with high nucleotide and amino acids homology to One Helix Protein (OHP) genes from Arabidopsis thaliana and Deschampsia antartica. The membrane spanning helix of InOHP is 91% identical to the MSH of the A. thaliana OHP. MSH is located in the C-terminal part of InOHP protein and corresponds to third MSH of LHC proteins. InOHP transcripts are numerous in leaves of plants when grown under continuous light and are also present in grown under flower inductive condition but their quantity is lower. Southern analysis showed that InOHP is member of a gene family involved in photoprotection of photosystems against excessive light |
format |
Article in Journal/Newspaper |
author |
Dąbrowska, Grażyna Barbara Dąbrowski, Henryk Paweł Richert, Agnieszka |
author_facet |
Dąbrowska, Grażyna Barbara Dąbrowski, Henryk Paweł Richert, Agnieszka |
author_sort |
Dąbrowska, Grażyna Barbara |
title |
Identification of the Ipomoea nil cDNA clone encoding protein with one transmembrane domain by differential display PCR |
title_short |
Identification of the Ipomoea nil cDNA clone encoding protein with one transmembrane domain by differential display PCR |
title_full |
Identification of the Ipomoea nil cDNA clone encoding protein with one transmembrane domain by differential display PCR |
title_fullStr |
Identification of the Ipomoea nil cDNA clone encoding protein with one transmembrane domain by differential display PCR |
title_full_unstemmed |
Identification of the Ipomoea nil cDNA clone encoding protein with one transmembrane domain by differential display PCR |
title_sort |
identification of the ipomoea nil cdna clone encoding protein with one transmembrane domain by differential display pcr |
publisher |
Uniwersytet Mikołaja Kopernika w Toruniu |
publishDate |
2020 |
url |
https://apcz.umk.pl/EQ/article/view/EQ.2020.015 |
genre |
antartic* |
genre_facet |
antartic* |
op_source |
Ecological Questions; Vol. 31 No. 2 (2020); 71-78 Ecological Questions; Tom 31 Nr 2 (2020); 71-78 1644-7298 |
op_relation |
https://apcz.umk.pl/EQ/article/view/EQ.2020.015/25395 https://apcz.umk.pl/EQ/article/view/EQ.2020.015 |
op_rights |
Prawa autorskie (c) 2020 Ecological Questions |
_version_ |
1766287807367610368 |