Western Blotting Using Strongyloides ratti Antigen for the Detection of IgG Antibodies as Confirmatory Test in Human Strongyloidiasis
The present study was conducted to evaluate the frequency of antigenic components recognized by serum IgG antibodies in Western blotting (WB) using a Strongyloides ratti larval extract for the diagnosis of human strongyloidiasis. In addition, the WB results were compared to the enzyme-linked immunos...
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ftunivtoronto:oai:localhost:1807/8122 2023-05-15T18:05:29+02:00 Western Blotting Using Strongyloides ratti Antigen for the Detection of IgG Antibodies as Confirmatory Test in Human Strongyloidiasis Luciana Pereira Silva Ivanildes Solange da Costa Barcelos Andréia Barcelos Passos-Lima Foued Salmen Espindola Dulcinéa Maria Barbosa Campos Julia Maria Costa-Cruz 2003-12-31 595950 bytes application/pdf http://hdl.handle.net/1807/8122 http://www.bioline.org.br/abstract?id=oc03141 en en_US eng Fundação Oswaldo Cruz, Fiocruz http://memorias.ioc.fiocruz.br; http://www.bioline.org.br/oc http://www.bioline.org.br/abstract?id=oc03141 Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 98 Num 5 http://hdl.handle.net/1807/8122 Copyright 2003 - Instituto Oswaldo Cruz - Fiocruz Strongyloides stercoralis - Strongyloides ratti - strongyloidiasis - immunodiagnosis - Western blotting Article 2003 ftunivtoronto 2020-06-17T11:09:15Z The present study was conducted to evaluate the frequency of antigenic components recognized by serum IgG antibodies in Western blotting (WB) using a Strongyloides ratti larval extract for the diagnosis of human strongyloidiasis. In addition, the WB results were compared to the enzyme-linked immunosorbent assay (ELISA) and the indirect immunofluorescence antibody test (IFAT) results. Serum samples of 180 individuals were analyzed (80 with strongyloidiasis, 60 with other intestinal parasitoses, and 40 healthy individuals). S. ratti was obtained from fecal culture of experimentally infected Rattus rattus. For IFAT, S. ratti larvae were used as antigen and S. ratti larval antigenic extracts were employed in WB and ELISA. Eleven S. ratti antigenic components were predominantly recognized by IgG antibodies in sera of patients with strongyloidiasis. There was a positive concordance for the three tests in 87.5% of the cases of strongyloidiasis. The negative concordance in the three tests was 94% and 97.5%, in patients with other intestinal parasitoses and healthy individuals, respectively. In cases of positive ELISA and negative IFAT results, diagnosis could be confirmed by WB. ELISA, IFAT, and WB using S. ratti antigens showed a high rate of sensitivity and specificity. In conclusion, WB using S. ratti larval extract was able to recognize 11 immunodominant antigenic components, showing to be a useful tool to define the diagnosis in cases of equivocal serology. Article in Journal/Newspaper Rattus rattus University of Toronto: Research Repository T-Space |
institution |
Open Polar |
collection |
University of Toronto: Research Repository T-Space |
op_collection_id |
ftunivtoronto |
language |
English |
topic |
Strongyloides stercoralis - Strongyloides ratti - strongyloidiasis - immunodiagnosis - Western blotting |
spellingShingle |
Strongyloides stercoralis - Strongyloides ratti - strongyloidiasis - immunodiagnosis - Western blotting Luciana Pereira Silva Ivanildes Solange da Costa Barcelos Andréia Barcelos Passos-Lima Foued Salmen Espindola Dulcinéa Maria Barbosa Campos Julia Maria Costa-Cruz Western Blotting Using Strongyloides ratti Antigen for the Detection of IgG Antibodies as Confirmatory Test in Human Strongyloidiasis |
topic_facet |
Strongyloides stercoralis - Strongyloides ratti - strongyloidiasis - immunodiagnosis - Western blotting |
description |
The present study was conducted to evaluate the frequency of antigenic components recognized by serum IgG antibodies in Western blotting (WB) using a Strongyloides ratti larval extract for the diagnosis of human strongyloidiasis. In addition, the WB results were compared to the enzyme-linked immunosorbent assay (ELISA) and the indirect immunofluorescence antibody test (IFAT) results. Serum samples of 180 individuals were analyzed (80 with strongyloidiasis, 60 with other intestinal parasitoses, and 40 healthy individuals). S. ratti was obtained from fecal culture of experimentally infected Rattus rattus. For IFAT, S. ratti larvae were used as antigen and S. ratti larval antigenic extracts were employed in WB and ELISA. Eleven S. ratti antigenic components were predominantly recognized by IgG antibodies in sera of patients with strongyloidiasis. There was a positive concordance for the three tests in 87.5% of the cases of strongyloidiasis. The negative concordance in the three tests was 94% and 97.5%, in patients with other intestinal parasitoses and healthy individuals, respectively. In cases of positive ELISA and negative IFAT results, diagnosis could be confirmed by WB. ELISA, IFAT, and WB using S. ratti antigens showed a high rate of sensitivity and specificity. In conclusion, WB using S. ratti larval extract was able to recognize 11 immunodominant antigenic components, showing to be a useful tool to define the diagnosis in cases of equivocal serology. |
format |
Article in Journal/Newspaper |
author |
Luciana Pereira Silva Ivanildes Solange da Costa Barcelos Andréia Barcelos Passos-Lima Foued Salmen Espindola Dulcinéa Maria Barbosa Campos Julia Maria Costa-Cruz |
author_facet |
Luciana Pereira Silva Ivanildes Solange da Costa Barcelos Andréia Barcelos Passos-Lima Foued Salmen Espindola Dulcinéa Maria Barbosa Campos Julia Maria Costa-Cruz |
author_sort |
Luciana Pereira Silva |
title |
Western Blotting Using Strongyloides ratti Antigen for the Detection of IgG Antibodies as Confirmatory Test in Human Strongyloidiasis |
title_short |
Western Blotting Using Strongyloides ratti Antigen for the Detection of IgG Antibodies as Confirmatory Test in Human Strongyloidiasis |
title_full |
Western Blotting Using Strongyloides ratti Antigen for the Detection of IgG Antibodies as Confirmatory Test in Human Strongyloidiasis |
title_fullStr |
Western Blotting Using Strongyloides ratti Antigen for the Detection of IgG Antibodies as Confirmatory Test in Human Strongyloidiasis |
title_full_unstemmed |
Western Blotting Using Strongyloides ratti Antigen for the Detection of IgG Antibodies as Confirmatory Test in Human Strongyloidiasis |
title_sort |
western blotting using strongyloides ratti antigen for the detection of igg antibodies as confirmatory test in human strongyloidiasis |
publisher |
Fundação Oswaldo Cruz, Fiocruz |
publishDate |
2003 |
url |
http://hdl.handle.net/1807/8122 http://www.bioline.org.br/abstract?id=oc03141 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_relation |
http://memorias.ioc.fiocruz.br; http://www.bioline.org.br/oc http://www.bioline.org.br/abstract?id=oc03141 Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 98 Num 5 http://hdl.handle.net/1807/8122 |
op_rights |
Copyright 2003 - Instituto Oswaldo Cruz - Fiocruz |
_version_ |
1766176951860461568 |